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Application of endophytic bacteria b22 in Sophora japonica in the control of root rot of Panax notoginseng

An endophytic bacteria, root rot technology, applied in bacteria, applications, microorganism-based methods, etc., can solve problems such as pollution, health threats, and pesticide residues in Panax notoginseng products, and achieve broad application prospects and strong inhibitory effects.

Active Publication Date: 2019-01-01
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even failure of Sanqi, and brought huge economic losses to the growers.
In order to control this fungal disease, a large amount of chemical pesticides are used, resulting in a large amount of pesticide residues in Panax notoginseng products, seriously polluting the environment, and greatly threatening people's health

Method used

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  • Application of endophytic bacteria b22 in Sophora japonica in the control of root rot of Panax notoginseng
  • Application of endophytic bacteria b22 in Sophora japonica in the control of root rot of Panax notoginseng
  • Application of endophytic bacteria b22 in Sophora japonica in the control of root rot of Panax notoginseng

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation, screening and identification of strains

[0023] 1. Separation of strains

[0024] Test materials: wild Vietnamese Sophora japonica collected from limestone areas in Tiandeng County, Guangxi.

[0025] Test strains: provided by the Institute of Plant Pathology, Guangxi University.

[0026] Medium: 1000ml NA medium: 3g beef extract, 1g yeast extract, 5g peptone, 10g sucrose, 15g agar, pH 7.0.

[0027] Surface disinfection: Rinse fresh and healthy Vietnamese Sophora japonica roots with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to clean the sediment, and then rinse the washed Vietnamese Sophora japonica roots with sterile water twice, and air dry the surface moisture , Move to the ultra-clean workbench, under aseptic conditions, soak the root of Sophora japonica with 75% ethanol in volume concentration for 1 min, rinse with sterile water 2 times, soak with sodium hypochlorite (effective chlorine 1%) for 2 min, and wash 3 times with sterile w...

Embodiment 2

[0079] Inhibitory effect of strain metabolites on root rot fungus of Panax notoginseng

[0080] 1. Fermentation culture of strain B22 and extraction of metabolites of strain B22

[0081] The endophyte B22 from Vietnam Sophora japonicus was inoculated into a 2 liter Erlenmeyer flask containing 1000ml of NB liquid medium (3g beef extract, 1g yeast extract, 5g peptone, 10g sucrose, pH 7.0), placed at a temperature of 28°C and rotating speed The fermentation was cultured in a shaker at 130r / min for 10 days. After the resulting fermented product was concentrated and dried under reduced pressure, the fermented product was added with 2 times methanol and ultrasonicated for 40 minutes with ultrasound. The ultrasound was centrifuged, and the supernatant obtained after centrifugation was concentrated under reduced pressure to obtain the strain The crude methanol extract of the fermented product is the metabolite of B22 endophytic bacteria in Sophora japonica.

[0082] 2. Inhibition of B22 met...

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Abstract

The invention discloses an endophytic bacterium B22 of Sophora japonica. The classification of the endophytic bacterium B22 of Sophora japonica is named Microbacterium sp. B22. The 16S rDNA gene sequence table of the strain is as described in SEQ ID NO.1, and the depository unit : General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, preservation date: September 29, 2015, preservation number: CGMCC No.11463 . For the first time, the present invention isolates and screens a strain of Sophora japonica endophytic bacteria (Microbacterium sp.) B22 from the root of the medicinal plant Sophora japonica. The field of biological control brings broad application prospects.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a Vietnamese Sophora japonicus endophytic bacteria B22 in the prevention and treatment of Panax notoginseng root rot. Background technique [0002] The prevention and control of plant diseases in modern agriculture relies too much on the use of chemical pesticides. The application of a large number of chemical pesticides not only has a long-term destructive effect on the ecological environment, but also causes a decline in the quality of agricultural products, excessive pesticide residues, drug resistance of pathogens, and harmful to humans and animals. Many problems. It is of great significance to find safer and more effective methods of disease control. The use of biological methods to control plant diseases can effectively solve the above problems. [0003] Panax notoginseng F.H.chen (Panax notoginseng F.H.chen), also known as Tianqi, Jinbuhuan, etc., has significant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12P1/04C12N1/205C12R2001/01
Inventor 黄荣韶蓝芳姚裕群李良波
Owner GUANGXI UNIV