Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests

A technology of Melaleuca alternifolia and essential oil, which is applied in the field of plant pesticides to prevent and control stored grain pests, to achieve outstanding fumigation effects, excellent control effects, and long-term effects

Active Publication Date: 2016-04-20
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of Melaleuca alternifolia essential oil as an inse...

Method used

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  • Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests
  • Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Application of Melaleuca alternifolia essential oil on the repelling effect of stored grain pests, assimilated corn beetle and corn weevil

[0024] 1. Insects to be tested

[0025] Miscellaneous corn beetle: The tested insects were provided by Huazhong Agricultural University in Wuhan, Hubei. Feed with flour / angie yeast (19 / 1), at a temperature of 30±2°C, a relative humidity of 75±5%, and dark conditions for indoor artificial rearing, continuous feeding for more than 5 generations, and select healthy adult worms for testing Insect source.

[0026] Corn elephant: The tested insects were provided by Anhui Agricultural University, Hefei City, Anhui Province. Feed with flour / angie yeast (19 / 1), at a temperature of 30±2°C, a relative humidity of 75±5%, and dark conditions for indoor artificial rearing, continuous feeding for more than 5 generations, and select healthy adult worms for testing Insect source.

[0027] 2. Melaleuca alternifolia essential oil for t...

Embodiment 2

[0038] Example 2: The application of Melaleuca alternifolia essential oil to the contact killing effect of stored grain pests Miscellaneous trichobeet and corn elephant

[0039] 1. Insects to be tested

[0040] The insects for testing are the same as those in Example 1.

[0041] 2. Melaleuca alternifolia essential oil for testing:

[0042] The essential oil for testing is the same as Example 1

[0043] 3. Test method

[0044] The filter paper membrane method was used. Put the filter paper with a diameter of 9 cm into a petri dish of the same diameter, and place a glass ring with an inner diameter of 8 cm and a height of 5 cm on the filter paper to prevent the test insects from climbing up. Dilute the essential oil with acetone into medicinal solutions of different concentrations, and use a pipette gun to pipette 1mL of the medicinal solutions of each concentration, drop them onto the filter paper to make the medicinal solution completely absorbed, and after the acetone is ...

Embodiment 3

[0048] Example 3: Application of Melaleuca alternifolia essential oil to the fumigation effect of stored grain pests, assimilated corn beetles and corn elephants

[0049] 1. Insects to be tested

[0050] The insects for testing are the same as those in Example 1.

[0051] 2. Melaleuca alternifolia essential oil for testing:

[0052] The essential oil for testing is the same as Example 1

[0053] 3. Test method

[0054] Use jar filter paper fumigation method. Put 30 test insects into a 300mL wide-mouth bottle, and the inner wall of the bottle is coated with polytetrafluoroethylene. Use a pipette gun to absorb different concentrations of essential oils and drop them on 2×3cm filter paper strips, and quickly hang the filter paper strips in the Erlenmeyer flask. After treatment, they were cultured in a constant temperature incubator at 30°C, and no reagent was added as a blank control. After 24, 48, and 72 hours, observe and record the death of test insects in each bottle, c...

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Abstract

Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests belongs to the technical field of biotechnology. According to the grain storage and production realities, the stored-grain insect pests (tribolium confusum and sitophilus zeamais) are repelled, touched out and fumigated. Compared with three essential oil single items and the mixing formula thereof, which are reported in the disclosed patent 201510340845.X, the melaleuca ahemifolia essential oil has a better control effect on the stored-grain insect pests (tribolium confusum and sitophilus zeamais), particularly has a remarkable fumigating insecticidal function, and is long in lasting period and low in probability of resistance to drugs. The melaleuca ahemifolia essential oil is rich in raw material source, low in cost and environmentally friendly, has the potential of being developed into a stored-grain insect pest fumigating agent, and has a very wide application prospect in controlling red-grain insect pests.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a plant pesticide for preventing and controlling stored grain pests. Background technique [0002] Food is the basis for human survival and an important factor for maintaining national stability. China is the largest agricultural country with the largest population in the world. Grain storage is one of the important means to rationally utilize grain resources, however, it is vulnerable to stored grain pests during storage, and the losses caused by stored grain pests are very common and serious. According to the statistics of relevant departments, due to the harm of stored grain pests, the loss of grain storage in my country's grain depots reaches 0.2% every year, while the loss of grain storage in rural areas is 8% to 10% due to poor equipment and poor control technology. Therefore, the improvement of grain storage technology and the control of stored grain pests are urgent. [000...

Claims

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Application Information

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IPC IPC(8): A01N65/28A01P7/04A01P17/00
CPCA01N65/00A01N65/28A01N2300/00
Inventor 操海群廖敏肖金京蒋兴川周丽君姚曦汤锋岳永德
Owner ANHUI AGRICULTURAL UNIVERSITY
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