Kit for directly detecting 71 type enterovirus and A16 type coxsackievirus and application thereof

Abstract

The invention provides a kit for directly detecting 71 type enterovirus and A16 type coxsackievirus and an application thereof. The kit comprises an antibody, a cell fixing solution, a PBS (phosphate buffer solution), a PBST (phosphate buffer solution twen-20), 1% Triton-X100, a cell reference sample, a preservative of sodium azide, an antibody stabilizer of BSA (bovine serum albumin), a fluorescent dye of Evans blue, a mounting medium, a slide carrier and a quality control slide, wherein the antibody contains a fluorescence marker. The kit has the advantages that the 71 type enterovirus and A16 type coxsackievirus in the sample cell can be directly detected; the advantages of flow cytometry and direct immunofluorescent technique on automation and high flux are realized, the virus can be quickly, sensitively and efficiently detected, the important significance is realized for the quick and accurate diagnosis of clinics, and different requirements of large hospitals and base hospitals can be met.

Description

technical field [0001] The invention belongs to the technical field of pathogen detection, and in particular relates to a kit for directly detecting enterovirus type 71 and coxsackievirus type A16 and an application thereof. technical background [0002] Hand, foot and mouth disease (hand, foot and mouth disease, HFMD) is an infectious disease caused by enteroviruses. There are more than 20 kinds of enteroviruses (types) that cause hand, foot and mouth disease, among which Coxsackievirus A16 (CA16) and enterovirus Virus type 71 (EV71) is the most common. It mostly occurs in children under 5 years old, manifested as mouth pain, anorexia, low fever, small herpes or small ulcers in hands, feet, mouth and other parts. Complications such as meningoencephalitis. Individual critically ill children develop rapidly and lead to death. [0003] The distribution of virus antigens in various tissues and organs after children infected with severe hand, foot and mouth disease caused by ...

AI Technical Summary

Problems solved by technology

However, the equipment is expensive, the requirements for the experimental site are high, the requirements for the operator are high, it is easy to pollute, and it is easy to produce false positives and lead to misdiagnosis

[0007] (2) Diagnosis by comprehensive evaluation of clinical symptoms: this method cannot be diagnosed accurately in the early stage of the disease due to atypical symptoms

IgG antibody needs to be produced at least 14 days after the onset of illness, and generally a second infection may be detected in sufficient amount, therefore, this method cannot be used for early diagnosis

IgM antibody is produced 5-7 days after virus infection, and is limited by serum IgM concentration, resulting in low sensitivity and prone to false negatives

During the window period from virus infection to IgM antibody production, it is not applicable to use serum immunity to detect virus antibodies, so the method of detecting serum IgM is also very limited

[0009] The above three methods have disadvantages such as inconvenient operation, long time-consuming, and inaccurate diagnosis during clinical use.

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