Health-care food with calcium supplementing function
A health food and food technology, which is applied in the direction of food ingredients, functions of food ingredients, and vitamin-containing food ingredients, etc. It can solve the problems of low calcium absorption rate and inability to prevent bone loss, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0011] The acquisition of embodiment 1 polypeptide
[0012] Wash and homogenize the bean worm with water, dissolve it in acetone to degrease, and make the suspension with water from the centrifuged residue, heat the suspension at 85°C for 10 minutes, adjust the pH value to 8 after cooling, add papain and trypsin Protease hydrolysis, the hydrolysis time is 3.2h, the temperature is 57°C, the pH value is 6.7, the enzyme dosage is 1.2%, the supernatant obtained by centrifugation or filtration is a polypeptide solution, and the polypeptide solution is treated with dextran G-50 (SephadexG-50) (1.5cm×90cm) separation, 20mmol / L HCl solution elution, flow rate 1.3mL / min, collect the eluted product, adjust the solution to pH 7.0, centrifuge at 10000 rpm for 15 minutes, and freeze the supernatant It was dried for later use; after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), bands with small molecular weight were recovered, and a total of 42 small peptide sequence...
Embodiment 2
[0028] The preparation of embodiment 2 food
[0029] By weight: 22 parts of vitamin K, 15 parts of walnut kernels, 15 parts of calcium carbonate, 5 parts of stone bark, 3 parts of magnesium lactate, 2 parts of whole milk powder, 3 parts of vitamin D, and 1 part of polypeptide A kind of polypeptide), 1 part of carboxymethyl cellulose, 1 part of magnesium stearate, mixed evenly, pulverized, sterilized, and packaged.
Embodiment 3
[0030] Example 3 Security Testing
[0031] The food prepared in Example 2 is verified for safety. According to the conventional verification methods in the art, it can be concluded that the present invention is confirmed to be safe and non-toxic by animal experiments. The experimental results and conclusions are as follows:
[0032] (1) The acute toxicity test shows that: according to the acute toxicity dose classification standard, it belongs to the actual non-toxic level;
[0033] (2) The results of bone marrow cell micronucleus test, sperm abnormality test and Ames test are negative;
[0034] (3) 30-day feeding test: the growth condition of the experimental animals was good, and compared with the control group, hematological examination, biochemical examination, main viscera ratio and histological examination results showed no abnormality, which proved that the present invention was safe and nontoxic.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com