Method for preparing stevioside

A technology of stevia and stevia leaves, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of large pollution, long production process route and high energy consumption, and achieves short process route and good economy. Benefit and low energy consumption

Inactive Publication Date: 2016-06-01
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the traditional production process of stevioside, the color of the extract obtained by soaking stevia leaves in water is dark brown, which puts a lot...

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0023] In the preparation method of stevioside of the present invention, comprise the following steps:

[0024] (1) Stevia leaves are soaked in tap water in a vacuum state, the solid-to-liquid ratio is 1:15-25, and the soaking time of stevia leaves is 40-60 minutes; the vacuum state is represented by "relative vacuum degree", and its value is between 0 to -101.325KPa; repeat soaking several times and mix the extracts;

[0025] (2) After soaking, filter and remove the stevia leaf waste residue under vacuum and flocculate, and the obtained solution enters the air after becoming a crude sugar solution;

[0026] (3) the crude sugar liquid is passed through a nanofiltration membrane with a molecular weight cut-off of 1KD to remove small molecular impurities and concentrate to obtain a concentrate;

[0027] (4) The concentrated solution is adsorbed, eluted, desalted and decolorized through a simulated moving bed separation system; the adsorption time is 8-12 hours, and eluted with ...

Embodiment 1

[0066]Take 10kg of stevia leaves, add 200kg of tap water, fully mix and stir with a stirring paddle in a vacuum tank with a relative vacuum of -70KPa, the stirring speed is 20rpm, and the time is 40min. Repeat four times, mix and transfer the four extracts To another leaching tank with the same relative vacuum, add chitosan for flocculation and filtration. The filtered extract is passed through a nanofiltration membrane with a molecular weight cut-off of 1KD in air to remove small molecule impurities and then concentrated. The concentrated solution enters the simulated moving bed for continuous adsorption, elution, desalination and decolorization. Then alcohol and small molecular impurities are removed through a nanofiltration membrane with a molecular weight cut-off of 300D and concentrated again. Finally, spray drying to obtain high-purity stevioside white powder. The liquid phase measurement results of the mixed extract liquid are: the extraction rate is 92.1%, and the li...

Embodiment 2

[0069] Take 10kg of stevia leaves, add 150kg of tap water, fully mix and stir with a stirring paddle in a vacuum tank with a relative vacuum of -80KPa, the stirring speed is 20rpm, and the time is 40min. Repeat four times, mix and transfer the four extracts To another leaching tank with the same relative vacuum, add chitosan for flocculation and filtration. The filtered extract is passed through a nanofiltration membrane with a molecular weight cut-off of 1KD in air to remove small molecule impurities and then concentrated. The concentrated solution enters the simulated moving bed for continuous adsorption, elution, desalination and decolorization. Then alcohol and small molecular impurities are removed through a nanofiltration membrane with a molecular weight cut-off of 300D and concentrated again. Finally, spray drying to obtain high-purity stevioside white powder.

[0070] The liquid phase measurement results of the mixed extract liquid are: the extraction rate is 97.4%, ...

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Abstract

The invention discloses a method for preparing stevioside. According to the method, oxidation reduction reaction of polyphenol oxidase and polyphenols in stevia rebaudiana leaves is inhibited by soaking and flocculating stevia rebaudiana leaves in a vacuum state, then generation of black substances can be prevented, the leaching rate of stevioside is increased, and the workload of removing pigment in a later procedure is greatly reduced. A simulated moving bed production process is adopted, continuous production is achieved, and the energy consumption is low; stevia rebaudiana leaf dregs generated after water extraction can be used as a raw material for extracting chlorogenic acid, so that relatively good economic benefits can be made, and relatively slight environment pollution can be caused; the method is short in whole production process route, low in energy consumption and small in pollution.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing stevioside. Background technique [0002] Stevioside is a new type of natural sweetener extracted from the stevia leaf of the Compositae herb. It has the characteristics of high sweetness and low calorie. Its sweetness is 200-300 times that of sucrose, but its calorific value is only 1 / 300 of that of sucrose. It is called "the third sugar source in the world". At present, stevioside has been widely used in food, medicine, agriculture and other fields in many countries and regions such as Asia, South America and the United States. Among them, China is one of the major stevioside producing countries in the world. [0003] Stevia contains nine kinds of sweet ingredients. Among them, the relative content of stevioside (ST), rebaudioside A (RA) and rebaudioside C (RC) is relatively high, accounting for more than 90% in total, and they are the m...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256Y02P20/10
Inventor 乔长晟吕爱弟马正旺钟海蛟
Owner 天津北洋百川生物技术有限公司
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