A sugar-responsive supramolecular gel with g-quadruplex structure and its preparation method
A technology of supramolecular gel and quadruplex, which is applied in the preparation of sugar derivatives, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., which can solve the difficulty of polymer synthesis and modification, and low loading rate and other problems, to achieve the effect of easy popularization and application, simple production process, and good sugar responsiveness
Inactive Publication Date: 2018-05-01
NANKAI UNIV
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Problems solved by technology
Using the polymer method, the synthesis and modification of the polymer are difficult, and there are problems such as low loading rate and fast release.
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[0029] A sugar-responsive supramolecular gel with a G-quadruplex structure, centered on the sugar response of 2-formylphenylboronic acid, using the ortho-diol structure of guanosine to form a dynamic covalent boron-ester bond , and then through the primary amine in tris (2-aminoethyl) amine and the aldehyde group of 2-formylphenylboronic acid to form a dynamic imine bond, the bases of guanine nucleosides form G- Tetrad square structure; the gel contains a non-covalent hydrogen bond and two dynamic covalent boron ester bonds, imine bonds, figure 1 Schematic diagram of the formation mechanism of G-quadruplex gel.
[0030] The preparation method of the sugar-responsive supramolecular gel with G-quadruplex structure, the steps are as follows:
[0031] 1) KCl and tris(2-aminoethyl)amine were prepared into a 10 mg / mL solution with purified water at room temperature and set aside.
[0032] 2) Add 7.06mg of guanosine and 3.75mg of 2-formylphenylboronic acid to a 5mL small test tube,...
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Disclosed is sugar response supramolecular gel with a G-quadruplex structure. A preparation method includes: taking sugar response of 2-formylphenylboronic acid as a center; utilizing a vicinal diol structure that vernine has to react with the 2-formylphenylboronic acid to form dynamic covalent bond boron ester bond; enabling primary amine in tri(2-amino ethyl)amine and aldehyde group of the 2-formylphenylboronic acid to form dynamic imine bond; forming the G-quartet structure when potassium ions among basic groups of vernine are stable. The sugar response supramolecular gel can be used for detecting release effect of methylthionine chloride in a glucose solution and an acidic solution. The sugar response supramolecular gel and the preparation method have the advantages that the gel prepared by the method is stable, high in strength, high in sugar response performance and capable of loading a lot of macromolecular / micromolecular gel; raw materials related to the preparation method are simple, and the supramolecular gel can be prepared by utilizing micromolecules while complex synthesis steps are not needed, so that cost is low, production process is simple, products can be stored for a long time without going bad, the raw materials are low in toxicity, and the preparation method is easy to popularize and apply.
Description
technical field [0001] The invention belongs to the field of biomedical materials, and relates to a sugar-responsive supramolecular gel with a G-quadruplex structure and a preparation method thereof. Background technique [0002] Diabetes has become one of the most serious diseases that endanger the health of Chinese people. The existing treatment method is to rely on daily subcutaneous injection of insulin to control the blood sugar concentration. There are two major problems in this method. One is that frequent injections not only cause irreversible damage to the injection site, but also bring physical pain and psychological shadow to the patient; Excessive amounts can cause hypoglycemia and lead to severe shock. Therefore, it is of great significance to develop a system that can control the release of insulin according to the intelligent response to the blood glucose level. [0003] After ionization, phenylboronic acid and its derivatives are very easy to combine with s...
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IPC IPC(8): C07H19/167C07H1/00A61K47/26A61K31/5415A61P3/10
CPCA61K31/5415A61K47/26C07H1/00C07H19/167
Inventor 马如江李圆凤刘勇史林启
Owner NANKAI UNIV