Desert oligotrophic bacterium DOB153 and application thereof to sand stabilization

An oligotrophic, desert technology, applied in microbial applications, the application of new oligotrophic bacteria in biological crusts to fix quicksand, can solve the problems of unseen oligotrophic bacteria application, rare reports of oligotrophic microorganisms, etc., and achieve growth rate Fast, strong growth and reproductive ability, and stable genetic characteristics

Inactive Publication Date: 2016-06-08
XINJIANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of literature search showed that, from an environmental point of view, the current oligotrophic bacteria are mainly researched on the water environment, but there are not many reports on oligotrophic microorganisms resistant to barren environments in dry desert areas, and there are no new discoveries similar to oligotrophic bacteria. A report on the application of vegetative bacteria in the fixation of quicksand in biological crusts

Method used

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  • Desert oligotrophic bacterium DOB153 and application thereof to sand stabilization
  • Desert oligotrophic bacterium DOB153 and application thereof to sand stabilization
  • Desert oligotrophic bacterium DOB153 and application thereof to sand stabilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation, screening and identification of oligotrophic bacteria Bacillussp.DOB153CGMCCNO.11926

[0021] 1. Isolation and screening of strains

[0022] The desert oligotrophic bacteria used in the present invention are sampled and isolated from the Gurbantunggut Desert in Junggar Basin, Xinjiang, and the strains capable of growing on LB medium are preliminarily screened out according to the analysis of the action mechanism of the bacteria. The microorganisms in the Gurbantunggut Desert in the Junggar Basin of Xinjiang were isolated by the traditional plate culture method, the strains were screened by primary screening and secondary screening, and the strains were purified by plate streaking method, and the bacterial strains were compared on the LB medium. In terms of growth and reproduction, a batch of well-growing microbial strains were screened out, and a desert oligotrophic bacterial strain numbered DOB153 with good comprehensive performance was selected f...

Embodiment 2

[0036] Example 2: Molecular identification of oligotrophic bacteria Bacillussp.DOB153CGMCCNO.11926

[0037] 1. PCR amplified DNA sequence of oligotrophic bacteria DOB153 and its sequencing

[0038] Pick a small amount of single colony of oligotrophic bacteria DOB153 strain, put it into an EP tube filled with 25 μL of sterile water, boil it at 100°C for 8-10 minutes, then quickly put it into the ice-water mixture for 5 minutes, centrifuge at 10000r / min, 5min, 4 Store at ℃, take the supernatant when used.

[0039] Determination of 16SrDNA gene sequence and construction of phylogenetic tree: The total DNA of oligotrophic bacteria DOB153 strain was extracted according to conventional methods, and the diluted universal primer was used for PCR amplification of 16SrDNA segment with deionized water.

[0040] The 50 μl reaction system contained: 5 μl of 10×PCR buffer, 20 pmol of each primer, 1 μl of template DNA, 0.5 U of TaqTM (TaKaRa Company), and 8 μl of dNTP. PCR amplification cond...

Embodiment 3

[0045] Example 3: Research on the characteristics of oligotrophic bacteria Bacillussp.DOB153CGMCCNO.11926

[0046] 1. Experimental process

[0047] The oligotrophic bacterium DOB153 was repeatedly streaked and activated on LB medium, and then used for the preparation of liquid bacterial agent. After inoculation, it was cultured in a shake flask at 37°C for 60 hours.

[0048] (1) Sand fixation test

[0049] Use quicksand to pack 2 disks with a length of 30cm × width 20cm × height 5cm, put the quicksand into the tray, and spray the oligotrophic bacteria DOB153 on the quicksand surface of one of the sand trays, and the other sand tray. An equal amount of tap water served as a control.

[0050] (2) Water retention test

[0051] Take 20 crucibles with known drying constant weight (wherein 10 are sprayed with water for comparison, and 10 are sprayed with bacterium solution of oligotrophic bacteria DOB153) and weighed, denoted as W 1 , respectively weighing 30g of sand into these...

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Abstract

The invention discloses a desert oligotrophic bacterium DOB153 and application thereof to sand stabilization. Through isolation, screening, selective breeding and acclimatization in Gurbantunggut Desert of Junggar Basin of Xinjiang, the oligotrophic bacterium Bacillus sp. DOB153 with the preservation number being CGMCC NO.11925 is obtained. The desert oligotrophic bacterium DOB153 and application thereof to sand stabilization have the advantages that after being used for the surface of the desert, a bacterial liquid prepared from the isolated oligotrophic bacterium DOB153 can play a role in sand stabilization, sand grain adhesion and quicksand activation control and provides good moisture conditions for germination of some plant seeds slow in germination to enable plants incapable of germinating due to insufficient moisture of soil to grow and develop, the number of major microorganisms in the soil can be increased effectively, major nutrient content of the soil can be increased, partial soil enzyme activity can be enhanced, algal crust can be recovered obviously, and accordingly the desert oligotrophic bacterium DOB153 is widely applicable.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to the technical field of the application of a new oligotrophic bacterium in biological crust fixing quicksand. Background technique [0002] Sandy desertification is a serious environmental problem faced by mankind today, and my country is one of the countries most seriously affected by desertification in the world. Environmental deterioration and economic poverty caused by desertification have seriously restricted ecological restoration and economic development, and governance is imminent. my country has carried out desertification research and prevention for half a century, but the desertification land has been expanding and spreading. Scientific research and prevention and control practices show that interdisciplinary and comprehensive integration is the only way to prevent and control desertification. At present, the main method of artificial sand fixation is to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/07
CPCC09K17/14C12N1/205C12R2001/07
Inventor 毛培宏吕杰张伶钱卫东
Owner XINJIANG UNIVERSITY
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