Polypeptide copper chelate prepared by using soybean protein isolate as substrate and preparation method

Abstract

The invention discloses a polypeptide copper chelate prepared by using soybean protein isolate as a substrate and a preparation method thereof. The soybean protein isolate is used as a basic material and is subjected to fermentation, enzymolysis, nanofiltration, chelation and drying, and the polypeptide copper chelate is prepared by using the polypeptide produced after fermentation, enzymolysis and filtration conducted on the soybean protein isolate as the a chelating agent. The polypeptide copper chelate is remarkably improved compared with like polypeptide chelated copper in purity and content, the mode that a pure microelement additive is directly added to a feed can be completely replaced, and an application effect is good. A food calling effect is good. A product is stable and contains a large amount of multifunctional polypeptide substances.

Description

technical field [0001] The invention relates to the field of feed production, in particular to a polypeptide copper chelate prepared with soybean protein isolate as a substrate and a preparation method thereof. Background technique [0002] Copper is one of the trace elements necessary for animals, and is the core element of various oxidase activators in crops, and plays an important role in catalyzing redox in organisms. Copper is also one of the elements necessary for blood formation. It can catalyze the combination of iron into the structure of heme and participate in the synthesis of various enzymes. It is an important component of many enzymes. The lack of copper will affect the utilization of iron by animals. , resulting in anemia (Xiong Jialin, 2001). Traditionally, the copper supplements used in pig feed are mainly inorganic copper (such as copper sulfate, copper chloride). , oxalic acid, phosphate ions) combine to form insoluble salts or precipitate out of the bod...

AI Technical Summary

Problems solved by technology

[0004] At present, there are methods of enzymatically hydrolyzing some protein waste or soybean meal and cottonseed meal to prepare peptides or small peptides to chelate copper, but there are many problems in these methods, which will eventually lead to product quality problems

For example, a patented small peptide chelated trace element feed additive and its production process (publication number: 102657285A), the amino acid in the hydrolyzed protein waste liquid used in it is unbalanced, and the heavy metals will exceed the standard, and the pH8.0 is used for chelation. The product contains A considerable part will exist in the form of copper hydroxide, and the spray drying temperature is too high, which will affect the product quality; the preparation method and application of a small peptide chelated copper complex are patented (publication number: 102827910A), which uses fermented enzymatic hydrolysis of soybean meal, etc. The protein raw material is chelated with metal salts afterward, no special treatment is needed in the later stage, the molecular weight distribution of the peptides in the mixture is wide, the production process is not easy to control, the product stability is poor, and the effective peptide content is low, etc. These problems need to be corrected urgently; the patent uses soybean meal as the A small peptide chelate prepared from a base material and its preparation method (publication number: 102669418A), which uses soybean meal as a substrate, and the content of the peptide obtained in the later stage is less, and the content of peptide chelated copper in the finished product is relatively low

[0005] The production process of polypeptide chelated copper produced by the prior art is relatively simple, the quality of the finished product is unstable, the content of chelated copper in the finished product is low, some products are of poor quality, the taste of the product is not good, and the price is expensive. These problems need to be solved urgently

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