One-step preparation method of manganese oxide nanometer contrast agent modified by polyethylene glycol for magnetic resonance

Abstract

The invention discloses a one-step preparation method of manganese oxide nanometer contrast agent modified by polyethylene glycol for magnetic resonance. 4mmol of manganese chloride tetrahydrate and 10ml of poly(ethylene glycol) bis(carboxymethyl) ether are stirred with magnetic stirring in a condition with nitrogen and are heated to 90-100 DEG C in order to be dissolved to a uniform solution; the solution is continuously heated to 140 DEG C, a reaction is carried out for 1 hour, the solution is heated to 180 DEG C, and a reaction is carried out for 4 hours; heating is stopped, after cooling to room temperature, polyethylene glycol molecules and manganese ions which are not reacted are removed by dialysis, and the water-soluble manganese oxide nanometer contrast agent modified by polyethylene glycol is obtained; and the manganese oxide nanometer contrast agent is stored at 4 DEG C. The one-step preparation method for preparing the manganese oxide nanometer contrast agent for magnetic resonance has simple steps and loose requirements of preparation conditions; the manganese oxide nanometer contrast agent prepared by poly(ethylene glycol) bis(carboxymethyl) ether which is used as a reaction solvent and a surfactant has the advantages of small particle size, uniform size, good dispersibility and stability; polyethylene glycol for surface modification reduces nonspecific adsorption of nano particles, the carried carboxyl is used for connection of targeting molecules, and the contrast agent has good biocompatibility.

Description

technical field [0001] The invention relates to a preparation method of a contrast agent, in particular to a one-step preparation method of a polyethylene glycol-modified manganese oxide magnetic resonance nano-contrast agent. Background technique [0002] Magnetic resonance imaging technology has been widely used in the field of biomedicine since it was proposed in 1973, and has become one of the essential tools in clinical diagnosis and basic research. Magnetic resonance molecular imaging uses magnetic resonance imaging technology to image specific molecules in the body, so as to achieve the purpose of early and specific diagnosis and curative effect monitoring of lesions (Shen Baozhong, Molecular Imaging (Second Edition), Beijing: People's Health Publishing House, 2010 : 2-413). In addition to the good tissue penetration and spatial resolution of magnetic resonance molecular imaging, the acquisition of magnetic resonance imaging signals does not depend on the separation ...

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Problems solved by technology

[0006] Aiming at the problems existing in the above-mentioned prior art, the present invention provides a one-step preparation method of a polyethylene glycol-modified manganese oxide magnetic resonance nano-contrast agent, which solves the problems of harsh experimental conditions and complicated operation steps in the traditional synthetic manganese oxide nano-contrast agent. defect

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