A kind of fermentation medium of bacillus amyloliquefaciens and application thereof

Abstract

The invention discloses a fermentation medium of bacillus amyloliquefaciens, which has a pH value of 6.8-7.8 and comprises 5-10 g / L of sucrose, 3-10 g / L of corn flour, 20-40 g / L of soybean meal powder and yeast powder 0-2g / L, dipotassium hydrogen phosphate 1-10g / L, potassium dihydrogen phosphate 1-5g / L, sodium hydroxide 0-2 g / L, magnesium sulfate 0-3g / L, calcium carbonate 0-2 g / L, manganese sulfate 0-1 g / L, ferric chloride 0-2g / L, urea 1-5g / L; It is also disclosed that it is used to prepare Bacillus amyloliquefaciens, including shake flask fermentation culture and seed tank culture. Large steps; the main raw materials of this fermentation medium are cheap agricultural and sideline products, which greatly reduces the production cost during large-scale fermentation, and can provide balanced nutrients for the fermentation of Bacillus amyloliquefaciens, so that Bacillus amyloliquefaciens can be fermented in a short period of time. A large number of multiplication in time and eventually the formation of spores.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a fermentation medium for increasing the biomass of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and shortening the fermentation cycle of the Bacillus amyloliquefaciens and a method for cultivating the Bacillus amyloliquefaciens using the fermentation medium. Background technique [0002] With the transformation of the aquaculture model to industrialization and intensification, the breeding density and feeding amount have also increased greatly, and the excrement of economic animals, the amount of residual bait, and the amount of pesticide application have also increased accordingly, and the pollution of aquaculture water has become more serious. The deterioration of water quality can also easily lead to the outbreak of economic animal diseases, accompanied by the extensive use of antibiotics and other drugs. [0003] As we all know, the application of...

AI Technical Summary

Problems solved by technology

At present, the optimization of the fermentation of Bacillus amyloliquefaciens at home and abroad is mainly focused on the production of antimicrobial lipopeptides, α-amylase, protease and other enzymes, and less research has been done on the optimization of the fermentation of Bacillus amyloliquefaciens. , the goal of most studies is to increase the number of live bacteria per unit volume of fermentation broth, and there are few studies on increasing the number of spores