Application of cactus polysaccharide extract to preparing central nervous system injury treating medicine
A technology of cactus polysaccharide and central nervous system, which is applied in the direction of nervous system diseases, drug combination, medical formula, etc., to achieve the effect of high active ingredient content, high product yield and less side effects
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Embodiment 1
[0029] The preparation of embodiment 1 cactus polysaccharide extract
[0030] (1) Wash the Mibangta cactus, slice it, dry it and crush it into a fine powder, pass through a 60-mesh sieve, add 2 times the weight of petroleum ether to the fine powder, reflux in a water bath for 2 hours, filter, discard the filtrate, and dry the powder Dry.
[0031] (2) Adding 25 times of pH by weight to the dried powder is a disodium hydrogen phosphate-citric acid buffer solution of 3.5, and then adds 0.3% cellulase and 0.7% pectinase accounting for the total weight of the mixed solution , fully stirred, enzymatic hydrolysis, after 2 hours in a water bath at 40°C, inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge, concentrate the solution into a clear paste, then add 80U / ml protease to the clear paste, shake at room temperature for 0.5h, Then heat to 100°C to inactivate the enzyme, cool to room temperature, centrifuge, and take the supernatant;
[0032] (3) adding ethano...
Embodiment 2
[0040] The preparation method of Milbangta cactus polysaccharide extract, its steps are as follows:
[0041] (1) Wash the Mibangta cactus, slice, dry and pulverize into fine powder, add 3 times the weight of n-hexane to the fine powder, reflux in water bath for 1 hour, filter, discard the filtrate, and degrease and depigment the powder to dry.
[0042] (2) adding 20 times of pH by weight in the dried mibangta cactus fine powder is 2 disodium hydrogen phosphate-citric acid buffer solution, then add the pectinase that accounts for 1% of the mixed solution gross weight, fully Stir, after 5 hours in a water bath at 30°C, inactivate the enzyme in a boiling water bath for 20 minutes, centrifuge, concentrate the solution into a clear paste, then add 100 U / ml protease to the clear paste, shake at room temperature for 2 hours, then heat and boil to inactivate the enzyme, Centrifuge and take the supernatant;
[0043] (3) adding ethanol to the supernatant so that the ethanol accounts f...
Embodiment 3
[0048] The preparation method of Milbangta cactus polysaccharide extract, its steps are as follows:
[0049] (1) Washing the cactus Mibangta, slicing, drying and pulverizing into fine powder, degreasing and depigmenting with cyclohexane, and then drying;
[0050] (2) adding 30 times of pH by weight in the dried mibangta cactus fine powder is 5 disodium hydrogen phosphate-citric acid buffer solution, and then adds the cellulase that accounts for 0.5% of the mixed solution gross weight, fully Stir, after 1 hour in a water bath at 60°C, inactivate the enzyme, centrifuge, concentrate the solution into a clear paste, then add 50U / ml protease to the clear paste, shake at room temperature for 0.5h, inactivate the enzyme, centrifuge, and take the supernatant;
[0051] (3) adding ethanol to the supernatant so that the ethanol accounts for 90% of the total volume, stirring and standing overnight, alcohol precipitation, and then drying the precipitate to obtain crude polysaccharide;
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