Application of cactus polysaccharide extract to preparing central nervous system injury treating medicine

A technology of cactus polysaccharide and central nervous system, which is applied in the direction of nervous system diseases, drug combination, medical formula, etc., to achieve the effect of high active ingredient content, high product yield and less side effects

Active Publication Date: 2016-07-13
汉济生物科技(武汉)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Mibangta cactus is an edible cactus introduced from Mexico by the Ministry of Agriculture of my country in 1998. It contains polysaccharides, flavonoids, sterols, pectin and other active ingredients, among which polysaccharides are one of the main active ingredients. There is no literature report about Mibangta Cactus polysaccharides have protective effects on central nervous system damage, especially drug-induced brain tissue damage

Method used

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  • Application of cactus polysaccharide extract to preparing central nervous system injury treating medicine
  • Application of cactus polysaccharide extract to preparing central nervous system injury treating medicine
  • Application of cactus polysaccharide extract to preparing central nervous system injury treating medicine

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Embodiment 1

[0029] The preparation of embodiment 1 cactus polysaccharide extract

[0030] (1) Wash the Mibangta cactus, slice it, dry it and crush it into a fine powder, pass through a 60-mesh sieve, add 2 times the weight of petroleum ether to the fine powder, reflux in a water bath for 2 hours, filter, discard the filtrate, and dry the powder Dry.

[0031] (2) Adding 25 times of pH by weight to the dried powder is a disodium hydrogen phosphate-citric acid buffer solution of 3.5, and then adds 0.3% cellulase and 0.7% pectinase accounting for the total weight of the mixed solution , fully stirred, enzymatic hydrolysis, after 2 hours in a water bath at 40°C, inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge, concentrate the solution into a clear paste, then add 80U / ml protease to the clear paste, shake at room temperature for 0.5h, Then heat to 100°C to inactivate the enzyme, cool to room temperature, centrifuge, and take the supernatant;

[0032] (3) adding ethano...

Embodiment 2

[0040] The preparation method of Milbangta cactus polysaccharide extract, its steps are as follows:

[0041] (1) Wash the Mibangta cactus, slice, dry and pulverize into fine powder, add 3 times the weight of n-hexane to the fine powder, reflux in water bath for 1 hour, filter, discard the filtrate, and degrease and depigment the powder to dry.

[0042] (2) adding 20 times of pH by weight in the dried mibangta cactus fine powder is 2 disodium hydrogen phosphate-citric acid buffer solution, then add the pectinase that accounts for 1% of the mixed solution gross weight, fully Stir, after 5 hours in a water bath at 30°C, inactivate the enzyme in a boiling water bath for 20 minutes, centrifuge, concentrate the solution into a clear paste, then add 100 U / ml protease to the clear paste, shake at room temperature for 2 hours, then heat and boil to inactivate the enzyme, Centrifuge and take the supernatant;

[0043] (3) adding ethanol to the supernatant so that the ethanol accounts f...

Embodiment 3

[0048] The preparation method of Milbangta cactus polysaccharide extract, its steps are as follows:

[0049] (1) Washing the cactus Mibangta, slicing, drying and pulverizing into fine powder, degreasing and depigmenting with cyclohexane, and then drying;

[0050] (2) adding 30 times of pH by weight in the dried mibangta cactus fine powder is 5 disodium hydrogen phosphate-citric acid buffer solution, and then adds the cellulase that accounts for 0.5% of the mixed solution gross weight, fully Stir, after 1 hour in a water bath at 60°C, inactivate the enzyme, centrifuge, concentrate the solution into a clear paste, then add 50U / ml protease to the clear paste, shake at room temperature for 0.5h, inactivate the enzyme, centrifuge, and take the supernatant;

[0051] (3) adding ethanol to the supernatant so that the ethanol accounts for 90% of the total volume, stirring and standing overnight, alcohol precipitation, and then drying the precipitate to obtain crude polysaccharide;

[...

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Abstract

The invention discloses an application of a cactus polysaccharide extract to preparing central nervous system injury treating medicine.After a cell proliferation experiment, it is found that by means of the cactus polysaccharide extract, neuronal cell proliferation can be promoted, neuron injuries caused by methylamphetamine can be reduced, mouse neurobehavior disorder caused by the methylamphetamine and learning and memory disorder caused by the methylamphetamine can be improved, neuron losses are reduced, and expression of a nerve growth factor (BDNF) can be improved.In this way, the cactus polysaccharide extract has the quite good protecting effect on neuron injuries, and can be used for treating central nervous system injuries.

Description

technical field [0001] The invention relates to the use of the cactus polysaccharide extract, especially the use in preparing medicine for treating central nervous system damage, and also relates to a preparation method of the cactus polysaccharide extract. Background technique [0002] Neurons, also known as nerve cells, are the basic units that constitute the structure and function of the nervous system, and have the functions of receiving, integrating and transmitting information. The pathological changes of various human central nervous system diseases include neuron damage, loss, necrosis and apoptosis. Protecting neurons by reducing neuron damage and loss and inhibiting neuron apoptosis is an important direction for the treatment of central nervous system injury diseases. [0003] In recent years, neuronal damage caused by drugs has gradually attracted the attention of researchers. Long-term use of "synthetic drugs" such as methamphetamine (MA) will lead to loose thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/33A61P25/28
CPCA61K36/33A61K2236/19A61K2236/331A61K2236/53A61K2236/55
Inventor 李超英
Owner 汉济生物科技(武汉)有限公司
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