DNA molecule for coding porcine alpha interferon and expression and purification method of recombinant protein of porcine alpha interferon
An interferon and coding technology, applied in the field of protein purification in the large-scale production of recombinant interferon, can solve the problems of complex steps and low antiviral activity of recombinant porcine alpha-interferon, and achieve a short purification period and disulfide bond. Correct pairing, cost-saving effect
Inactive Publication Date: 2016-07-13
北京大北农科技集团股份有限公司动物医学研究中心 +3
5 Cites 2 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Abstract
The invention relates to the field of biological pharmacy, and mainly relates to a DNA molecule for coding porcine alpha interferon and an expression and purification method of a recombinant protein of the porcine alpha interferon. Recombinant escherichia coli BL21/pET-21a-rPoIFN[alpha] is taken as a production bacterial strain, an expression protein of which has a coding sequence optimized through a codon bias in escherichia coli and is suitable for expression in escherichia coli. According to the expression and purification method, plenty of target protein in an inclusion body form can be obtained in precipitation of broken bacteria, and a large amount of recombinant porcine alpha interferon with high purity can be obtained through inclusion body denaturation, renaturation, affinity chromatography, and dialysis displacement of a buffer. The recombinant porcine alpha interferon is high in biological activity, and is convenient to apply with reduced production cost.
Application Domain
BacteriaPeptide preparation methods +4
Technology Topic
Expression proteinAlpha interferon +14
Image
Examples
- Experimental program(2)
- Effect test(1)
Example Embodiment
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Similar technology patents
Comprehensive method for processing laterite nickel ore to produce electrolytic nickel through full wet method
Owner:YUNNAN TIN GROUP HLDG
Power-interrupting-free shifting speed changing box of electric vehicle and shifting control method of power-interrupting-free shifting speed changing box
Owner:青岛莱吉传动系统科技有限公司
Steel plate with high strength and low yield ratio
Owner:BAOSHAN IRON & STEEL CO LTD
Classification and recommendation of technical efficacy words
- reduce manufacturing cost
Local grain anti-counterfeiting print
Owner:HAINAN PPK NETWORK TECH
Humic acid multifunctional degradable black mulch film
Owner:SHANDONG UNIV OF SCI & TECH
Method for welding specially-thick high-strength bridge steel plate
Owner:SHOUGANG CORPORATION
Method for preparing porous nano carbon materials with biomass serving as carbon source
Owner:HEILONGJIANG UNIV