Peltate yam rhizome endophyte for generating saponinase and application

Abstract

The invention discloses peltate yam rhizome endophyte for generating saponinase and application. The peltate yam rhizome endophyte is Aspergillus flavus strain SYfx213.2 and preserved in CGMCC, and the preservation number of the peltate yam rhizome endophyte is CGMCC 11517. The peltate yam rhizome endophyte can be used for industrial fermentation and production of peltate yam rhizome saponinase, the yield of saponinase can be improved, and the market requirement is met.

Description

technical field [0001] The invention relates to a plant endophytic bacterium and its application, in particular to a saponinase-producing endophytic bacterium of Dioscorea scutellaria and its application. Background technique [0002] Dioscorea shield leaf is a medicinal plant, its rhizome contains 1.1% to 16.15% diosgenin, about 45% starch, 40% cellulose and some water-soluble glycosides, alkaloids, flavonoid glycosides Chemical components such as glycosides, cardiac glycosides, alkaloids, tannins, and pigments. Among them, saponin is the main active ingredient of Dioscorea, a steroidal saponin, commonly known as saponin, saponinase is found in the rhizome of Dioscorea shield leaf plant, when the rhizome tissue is crushed into powder or ground into pulp, saponin The enzyme is released and contacts with diosgenin to produce enzymatic hydrolysis, and the diosgenin undergoes deglycosylation reaction and is transformed into diosgenin. The diosgenin produced by the hydrolysis ...

AI Technical Summary

Problems solved by technology

Among them, the acid hydrolysis method prepares diosgenin, and the yield is low, only about 2%

During the natural fermentation process, a variety of microorganisms participate in the fermentation, and the fermentation conditions are difficult to control. The increase of impurities will reduce the quality of diosgenin, and the process is not ideal

The enzymatic hydrolysis method is to crush the dry material of yam and add a certain amount of cellulase, pectinase, amylase, amygdalinase and glucosidase to the enzyme solution for enzymolysis, after suction filtration, acid hydrolysis, alkali neutralization, Washing and suction filtration, drying a series of processes and then extracting and concentrating to obtain diosgenin, although the yield is greatly improved compared with direct acid hydrolysis, the increase rate is 28.8%, but it is still not ideal

At the same time, the production process of extracting sapogenin from yams will produce a large amount of waste water, with an average of 450 tons of waste water discharged per ton of sapogenin produced. These waste waters have high concentrations of organic pollutants, high acidity, and high chroma, and are extremely difficult to treat.

And as the demand for saponin in the pharmaceutical industry increases day by day, it stimulates the high-speed and unrestrained utilization of wild Dioscorea scutellaria, and the wild resources are gradually exhausted; Cyclic planting-digging will cause serious damage to the fragile ecological environment of its production areas (mainly mountainous areas); on the other hand, long-term artificial planting will cause the degradation of diosmin germplasm resources, and its saponin content will continue to decrease