Application of acid phosphatase and related biological materials thereof in constructing phosphate-solubilizing engineering bacteria

A phosphohydrolase and phosphorus-dissolving technology, applied in microorganism-based methods, applications, microorganisms, etc., can solve the problems of many by-products and difficult purification.
CN105779408AActive Publication Date: 2016-07-20INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Publication Date
2016-07-20

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Abstract

The invention discloses application of acid phosphatase and related biological materials thereof in constructing phosphate-solubilizing engineering bacteria. The application refers to application of protein as shown in a) or b) or c) or d) in phosphohydrolase: a) protein constituted by amino acid sequences on 1st-203rd sites of SEQ ID N0.2; b) protein constituted by amino acid sequences on 26th-203rd sites of SEQ ID N0.2; c) fusion protein obtained by fusing a protein tag / protein tags on carboxyl terminal (C terminal) or / and amino terminal (N terminal) of the protein of a) or b); and d) protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of an amino acid sequence as shown in SEQ ID No.2 or SEQ ID No.6 and has the activity of the acid phosphatase. According to the application disclosed by the invention, biological engineering bacteria, which can effectively activate soil phosphorus nutrients, can be cultivated.
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Description

technical field

[0001] The invention relates to the use of acid phosphatase and related biological materials in the biological field, in particular to the application of acid phosphatase and related biological materials in building phosphorus-dissolving engineering bacteria. Background technique

[0002] Phosphorus is one of the three essential elements for plant growth and development, and plays an important role in the life process. The phosphorus used by plants mainly comes from the soil. At present, 2 / 3 of the cultivated land in my country is deficient in phosphorus. The main reason for phosphorus deficiency is the insufficient content of available phosphorus in the soil. About 95% of phosphorus is insoluble and ineffective phosphorus, which is difficult for plants to absorb and utilize. my country consumes about 10.5-12 million tons of phosphate fertilizers every year (2010 China Chemical Industry Information Network), but the plant utilization rate of phosphate fertil...

Claims

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