Preparation and application of MIP (molecularly imprinted polymer) with tropane alkaloid drug specificity

A tropane alkaloid, molecular imprinting technology, applied in ion exchange, ion exchange regeneration, chemical/physical process and other directions, can solve the problem of inability to enrich multiple alkaloids at the same time, achieve excellent adsorption performance, and simple preparation steps , the effect of low cost

Inactive Publication Date: 2016-07-27
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first object of the present invention is to provide a molecularly imprinted polymer specific for tropane alkaloid drugs, which can realize specific recognition, separation, Enrichment and purification, and can solve the defect that the existing technology cannot enrich multiple alkaloids at the same time

Method used

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  • Preparation and application of MIP (molecularly imprinted polymer) with tropane alkaloid drug specificity
  • Preparation and application of MIP (molecularly imprinted polymer) with tropane alkaloid drug specificity
  • Preparation and application of MIP (molecularly imprinted polymer) with tropane alkaloid drug specificity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A method for preparing a molecularly imprinted polymer specific for tropane alkaloid drugs mainly includes the following steps:

[0058] 1), dissolving anisodine in acetonitrile, adding methacrylic acid for pre-polymerization to form a host-guest complex;

[0059] 2) After the pre-polymerization is completed, trimethylolpropane trimethacrylate and azobisisobutyronitrile are added, an inert gas is passed into the obtained mixed solution for protection and sealing, and the water bath is shaken to carry out polymerization reaction to obtain molecularly imprinted polymerization thing;

[0060] 3) Grinding and sieving the molecularly imprinted polymer obtained in step 2); eluting with an organic solution until no template molecule can be detected in the eluent, washing the residual organic solution with methanol, and drying to obtain the Tr Alkane alkaloid drug-specific molecularly imprinted polymers.

[0061] Wherein, the mol ratio that anisodine and methacrylic acid add ...

Embodiment 2

[0066] A method for preparing a molecularly imprinted polymer specific for tropane alkaloid drugs mainly includes the following steps:

[0067] 1) Dissolve anisodine in acetonitrile, add methacrylic acid, conduct pre-polymerization with ultrasound at 18°C ​​for 40 minutes, and the frequency of ultrasound is 120 Hz to form a host-guest complex;

[0068] 2) After the pre-polymerization is completed, add trimethylolpropane trimethacrylate and azobisisobutyronitrile, and pass nitrogen gas into the obtained mixed solution for 5 minutes until the deoxidation is complete. After sealing, shake in a water bath at 65°C and perform polymerization at 800rpm After 22 hours, the molecularly imprinted polymer was obtained.

[0069] 3) Grinding the molecularly imprinted polymer obtained in step 2), passing through a 700-mesh sieve, and eluting with 10% acetic acid methanol solution (the volume ratio of acetic acid and methanol is 3:7) until no template can be detected in the eluent After the...

Embodiment 3

[0075] A method for preparing a molecularly imprinted polymer specific for tropane alkaloid drugs mainly includes the following steps:

[0076] 1) Anisodine was dissolved in acetonitrile, methacrylic acid was added, and ultrasonic prepolymerization was carried out at 22°C for 25 minutes at a frequency of 180 Hz to form a host-guest complex;

[0077] 2) After the pre-polymerization is completed, add trimethylolpropane trimethacrylate and azobisisobutyronitrile, and pass nitrogen gas into the obtained mixed solution for 10 minutes until the deoxidation is complete. After sealing, shake in a water bath at 55°C and perform polymerization at 1200rpm After 26 hours, the molecularly imprinted polymer was obtained.

[0078] 3), grind the molecularly imprinted polymer obtained in step 2), pass through a 900-mesh sieve, and elute with 20% acetic acid methanol solution (the volume ratio of acetic acid and methanol is 3:7) until no template can be detected in the eluent After the molecul...

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Abstract

The invention relates to the field of drug extraction, separation and purification, in particular to an MIP (molecularly imprinted polymer) with tropane alkaloid drug specificity. The MIP is mainly prepared from anisodine, methacrylic acid, trimethylolpropane trimethacrylate, 2,2'-azobis(2-methylpropionitrile) and a pore-foaming agent through a reaction, wherein the adding mole ratio of anisodine to methacrylic acid is 1:4-1:8, the adding mole ratio of trimethylolpropane trimethacrylate to methacrylic acid is 4:1-6:1, and the adding mole ratio of 2,2'-azobis(2-methylpropionitrile) to methacrylic acid is 15 mg:1 mmol-25 mg:1 mmol. Through selection of kinds and dosage of a solvent, a functional monomer, a crosslinking agent and an initiator and optimization of polymerization conditions, the MIP with better particle size distribution, physical stability and excellent adsorption is prepared, can selectively recognizing four tropane alkaloids and has good class specificity.

Description

technical field [0001] The invention relates to the field of drug extraction and separation and purification, in particular to the preparation and application of a tropane alkaloid drug-specific molecularly imprinted polymer. Background technique [0002] Alkaloids are a class of nitrogen-containing basic organic compounds that widely exist in nature. They are active ingredients of plant-derived traditional Chinese medicines and have significant physiological activities, mainly manifested in anticancer, antitumor, antiviral, antibacterial, and anti-inflammatory effects. Generally, there are many types of alkaloids, among which tropane alkaloids are the main components of many plant-derived traditional Chinese medicines to exert clinical efficacy. These tropane alkaloids mainly include hyoscyamine, scopolamine, anisodamine, and anisodamine , often co-exist in botanical traditional Chinese medicines. Due to their low content, the extraction and separation of this type of alkal...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/06B01D15/08B01D11/02
CPCC08F222/14B01D11/02B01D15/08
Inventor 佘永新王静曾绍梅王珊珊金茂俊邵华金芬郑鹭飞
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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