Lactic acid bacteria group and its application in the preparation of straw feed
The technology of lactic acid bacteria group and straw is applied in the application field of straw feed preparation, which can solve the problems of low bioavailability, many varieties of natural microorganisms, and insignificant inhibitory effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0075] Isolation and Identification of Lactic Acid Bacteria L.panis and L.plantarum
[0076] Inoculate the Trichoderma reesei strain on Sandcastle's medium, shake at 28°C and 200rpm for 2 days, filter out the hyphae and wash them with sterile water for 3 times.
[0077] Take 10 g of rice straw after gas explosion, add 2 g of washed mycelia, cultivate in a constant temperature incubator at 28° C., maintain a relative humidity of 80-85%, and add 5 ml of sterile water every 24 hours. After six days of culture, the culture was continued for 7 days in an anaerobic environment at 37°C. Take 2g of the treated yellow rice straw sample, add 20ml of water, shake vigorously, filter with four layers of gauze, dilute the filtrate 1000 times, take 100μL and apply it on the MRS solid medium, and after anaerobic cultivation at 30℃ for 48h , to pick colonies.
[0078] Colonies were picked and placed in 5ml MRS broth medium, anaerobically cultured at 30°C for 48h, diluted, and then spread on ...
Embodiment 2
[0080] Changes of rice straw fiber during air-exploded rice straw yellow storage
[0081] In order to observe the changes of the straw fiber during the yellow storage process of the air-exploded rice straw, the samples of the anaerobic culture at 37°C for 7 days in Example 1 were taken, and the slices were made of the air-exploded rice straw, and the changes of the straw yellow storage fiber were observed with a transmission electron microscope. .
[0082] figure 1 Shown is the fiber structure of the rice straw after gas explosion, and the structure is generally flat.
[0083] figure 2 The fiber structure of air-exploded rice straw treated with Trichoderma reesei is shown in , with some incomplete hyphae attached to the fiber structure, and obvious hole-like structures appear between the fibers.
[0084] image 3 Shown is the microstructure of the air-exploded rice straw treated with Trichoderma reesei and added with yellow storage for 7 days. It can be seen that the fibe...
Embodiment 3
[0086] Application of Lactic Acid Bacteria in Air-exploded Rice Straw Yellow Storage
[0087] The lactic acid bacteria were inoculated in the MRS liquid medium and cultured at 30°C for 3 days, and the pure lactic acid bacteria L.fermentum and L.plantarum were used as comparison strains for the same treatment.
[0088] Take 2.5 g of rice straw after gas explosion, and add 75 FPU Trichoderma reesei fermentation liquid (about 1.25 ml). After pre-cultured lactic acid bacteria (L.panis and L.plantarum flora; Lactobacillus fermentum L.fermentum and L.plantarum flora) and pure lactic acid bacteria (L.fermentum; L.plantarum flora) centrifuged to discard the culture medium, use After diluting with sterile water to 0.1OD, take 1.25mL and add them to the system respectively, and add 1.25mL of water to maintain the same water content of the straw. Each experimental group was replicated three times, at 30°C, sealed cultured for 30 days, and then measured the nutritional components.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More