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Preparation method of nanoparticles with photothermal effect and application thereof

A nanoparticle and photothermal effect technology, applied in the field of nanomedical materials, can solve the problems of short half-life, irreversible degradation, loss of photothermal conversion ability, etc.

Inactive Publication Date: 2016-08-03
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, indocyanine green is the only molecule with near-infrared properties approved by the FDA that can be used for clinical diagnosis. However, its application in tumor treatment is still limited: 1. Photobleaching, accelerated quenching under light in aqueous solution; 2. High temperature significantly accelerates thermal degradation in aqueous solution; 3. Unstable in water, concentration-dependent aggregation, irreversible degradation, etc., optical properties change, and even loss of photothermal conversion ability; 4. Short half-life, easy to combine with serum protein liver clearance

Method used

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  • Preparation method of nanoparticles with photothermal effect and application thereof
  • Preparation method of nanoparticles with photothermal effect and application thereof
  • Preparation method of nanoparticles with photothermal effect and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of PEGylated Chitosan Genipin Nanoparticles

[0034] 1) Cyclohexane 18.15mL is the oil phase, tritonX-1004.74g is the surfactant, n-octanol 4.41mL is the co-surfactant, and mixed and stirred for 30min.

[0035] 2) Chitosan 33mg was dissolved in water and ultrasonically vortexed to form a 0.55mL solution, wherein the molar number of primary amino groups of chitosan was 0.183mmol, genipin 27mg was dissolved in 200ul ethanol, and then deionized water was added to form a 0.55mL solution, The above two solutions were first mixed and ultrasonically cross-linked for 2 minutes to prepare an aqueous phase.

[0036] 3) The aqueous phase solution in step 2) was added to the oil phase containing surfactant and co-surfactant in step 1), ultrasonicated for 10 min to form an inverse microemulsion (1), and reacted at room temperature for 12 h.

[0037] 4) 50 mg of succinimide acetate PEG was dissolved in 0.55 mL of aqueous solution, and added to the oil phase...

Embodiment 2

[0039] Example 2: PEGylated Chitosan Genipin Nanoparticles Loaded Indocyanine Green and Characterization of Its Properties

[0040] 1) Dissolve indocyanine green, a photothermal conversion material, in deionized water, and disperse it ultrasonically to form a 1 mg / mL solution. Take 1 mL of the above indocyanine green solution and add it dropwise to 1 mL of 2 mg / mL PEGylated chitosan genipin nano Microparticles, 100w ultrasonic 10min, stirred overnight 12h, using 10KDa ultrafiltration tube ultrafiltration to remove excess free indocyanine green.

[0041] 2) Transmission electron micrograph ( figure 1 ) shows that the prepared nanoparticles are spherical, with an average particle size of about 5 nanometers, all within 10 nanometers.

[0042] 3) Fourier transform infrared absorption spectrum ( figure 2 ) showed that PEG molecules were successfully modified to the surface of polysaccharide genipin nanoparticles.

[0043] 4) Dynamic light scattering analysis ( image 3 ) shows t...

Embodiment 3

[0045] Example 3: PEGylated chitosan genipin-indocyanine green nanoparticles cell killing effect

[0046] Take U87 cells 10 4 Inoculate in a 96-well plate and incubate overnight, take PEGylated chitosan genipin-indocyanine green nanoparticles and free indocyanine green and other gradient dilutions, add 10ul to DMEM medium, and form ICG with final concentrations of 4.68 and 9.375 , 18.75, 37.5, 17.5ug / mL culture solution, 5% CO 2 , incubated at 37° for 4 hours, irradiated with laser light in a 37° water bath for 15 minutes, discarded the above culture medium, washed it with PBS, added normal culture medium, and detected cell activity by MTT method. Figure 5 The results show:

[0047] 1) The polysaccharide genipin-indocyanine green nanoparticles are under no laser irradiation, when the indocyanine green concentration is 75ug / mL, the cell activity is higher than 70%, indicating that the nanoparticles are less toxic to cells; Under the irradiation of (808nm, 0.17W / cm 2 , 10mi...

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Abstract

The invention provides a preparation method of nanoparticles with a photothermal effect and an application thereof. The nanoparticles are prepared by the following steps: positively charged molecules such as chitosan and the like undergo a polymerization reaction triggered by the use of genipin in a reversed-phase microemulsion; the surface is PEG-modified to enhance biocompatibility; and finally, a photothermal conversion material is supported under the positive and negative charge effect. The obtained nanoparticles have remarkable photothermal effect, can be applied in photo-thermal therapy of tumor cells, and can kill tumor cells by strong photo-thermal killing capability and inhibit tumor growth.

Description

technical field [0001] The invention relates to the field of nano medical materials, in particular to a preparation method and application of nano particles with photothermal effect. Background technique [0002] Photothermal therapy (photothermal therapy, PTT) is a minimally invasive tumor treatment technology, that is, the laser is irradiated to the tumor site, and the light energy is converted into heat energy to increase the temperature of the tumor site and kill the tumor. Near-infrared photothermal therapy is to inject or implant a photothermal agent locally in the tumor to improve photosensitivity. After a specific wavelength of laser radiation, the photothermal agent quickly converts light and heat into heat energy to kill the tumor. The method reduces systemic toxicity, has the advantages of low trauma and easy operation, and can also be used for carrier-modified targeted therapy, which has a good application prospect. Photosensitizers used in photothermal therapy ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/14A61K47/36A61K47/34A61P35/00
Inventor 谭明乾宋小杰马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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