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Method for preparing modified cellulose immobilized lipase

A technology for immobilizing lipase and cellulose, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve the problem of reducing the binding capacity and reaction capacity of lipase, complex separation and purification operations, It is not easy to reuse and other problems, to achieve the effect of unique and novel method, high activity recovery rate and less loss of enzyme activity

Inactive Publication Date: 2016-08-03
CHANGZHOU C PE PHOTO ELECTRICITY SCI & TECHN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: for the current often directly using free lipase as a catalyst, it has poor operational stability, easy inactivation, difficult re-use, complex separation and purification operation, and the traditional immobilization method of immobilized lipase Most of the lipases are immobilized on high-cost insoluble polymers or inorganic carriers. The presence of the carrier will "dilute" the activity of the lipase and reduce the binding capacity and reaction ability of the lipase. A bamboo shoot as raw material , the bamboo shoot fiber was prepared by acid-soluble enzymolysis, then alkalized with sodium hydroxide, oxidized and modified its surface groups under the catalysis of hydrogen peroxide and copper, finally impregnated and solidified by vacuum freeze-drying, so as to obtain modified cellulose Preparation method of immobilized lipase

Method used

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Examples

Experimental program
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Effect test

example 1

[0020] First, weigh 1 kg of bamboo shoots, peel the skins, take the cores and cut them into small pieces with a particle size of 3mm, put them in an oven and dry them at 105°C for 12 hours, put the dried bamboo shoots into a jet mill, and pass them through a 100-mesh standard sieve , to obtain bamboo shoot powder; then pour 400g of bamboo shoot powder into a 0.1mol / L hydrochloric acid solution with a concentration 5 times its volume, place it on a shaker and oscillate and soak overnight, remove acid-soluble impurities and transfer it to a Buchner funnel, and pump it with deionized water Filter and wash 3 times, and naturally air-dry to obtain the pretreated bamboo shoot powder; then put the above-mentioned pretreated bamboo shoot powder and the composite enzyme preparation together in a ceramic fermenter in a mass ratio of 100:1, and then add the total volume of both 3 times of deionized water, stirred evenly, put it into an incubator, and kept it at 40°C for enzymolysis for 4 ...

example 2

[0023]First, weigh 1.5kg of bamboo shoots, peel the skins, take the bamboo shoot cores and cut them into small pieces with a particle size of 4mm, put them in an oven and dry them at 108°C for 14 hours, put the dried bamboo shoots into a jet grinder and crush them and pass through a 100-mesh standard Sieve to obtain bamboo shoot powder; then pour 450g of bamboo shoot powder into 0.1mol / L hydrochloric acid solution with a concentration of 7 times its volume, place it on a shaker and oscillate and soak overnight, remove acid-soluble impurities and transfer it to a Buchner funnel, and use deionized water Suction filtration and washing 4 times, natural air drying to obtain the pretreated bamboo shoot powder; then put the above pretreated bamboo shoot powder and the composite enzyme preparation together in a ceramic fermenter in a mass ratio of 100:1, and then add the total volume of the two 4 times the amount of deionized water, stirred evenly, put it into an incubator, and kept it...

example 3

[0026] First, weigh 2kg of bamboo shoots, peel the skins, take the cores and cut them into small pieces with a particle size of 5mm, put them in an oven and dry them at 110°C for 15 hours, put the dried bamboo shoots into a jet mill, and pass them through a 100-mesh standard sieve , to obtain bamboo shoot powder; then pour 500g of bamboo shoot powder into 0.1mol / L hydrochloric acid solution with a concentration 8 times its volume, place it on a shaker and oscillate and soak overnight, remove acid-soluble impurities and transfer it to a Buchner funnel, and pump it with deionized water Filter and wash 5 times, and naturally air-dry to obtain the pretreated bamboo shoot powder; subsequently, the above-mentioned pretreated bamboo shoot powder and the composite enzyme preparation are packed into a ceramic fermenter together in a mass ratio of 100:1, and then the total volume of the two is added 5 times of deionized water, stirred evenly, put it into an incubator, and kept it at 50°C...

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Abstract

The invention discloses a preparation method of modified cellulose immobilized lipase, and belongs to the field of lipase preparation. In the invention, bamboo shoots are used as raw materials, and bamboo shoot fibers are prepared by acid-soluble enzymolysis, then alkalized with sodium hydroxide, oxidatively modified by the catalysis of hydrogen peroxide and copper, and finally impregnated and solidified by vacuum freeze-drying. Thereby, the preparation method of the modified cellulose immobilized lipase is obtained. The method of the invention is unique and novel, and the preparation process is simple and feasible. It not only utilizes the spatial structure and flexibility of cellulose, but also has a large number of free hydroxyl groups. The functional group is covalently bonded, has excellent compatibility, and does not affect the activity and reaction ability of the enzyme, improves the binding rate and specific activity of the enzyme, has a huge binding capacity, and is suitable for large-scale application.

Description

technical field [0001] The invention discloses a preparation method of modified cellulose immobilized lipase, which belongs to the field of lipase preparation. Background technique [0002] As a biocatalyst, enzymes have the advantages of mild reaction conditions, biodegradability, low energy consumption, low raw material requirements, and high-quality finished products compared with chemical catalysis. The chemical essence of an enzyme is a protein, and its catalytic properties strictly depend on the three-dimensional structure of the protein and the accurate functional positioning of the substrate and the active group of the protein. Free enzymes usually survive in a narrow range of temperature and pH value, are easily denatured and inactivated, are difficult to reuse, and cannot achieve continuous operation. For modern industry, free enzyme is not an ideal catalyst, which greatly limits the application of enzyme. After the enzyme is immobilized, the purification process...

Claims

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Application Information

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IPC IPC(8): C12N11/12C08B15/02
CPCC12N11/12C08B15/02C12N9/18C12Y301/01003
Inventor 郭伟明张明林茂平
Owner CHANGZHOU C PE PHOTO ELECTRICITY SCI & TECHN
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