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A fluorescence probe, a preparing method thereof and applications of the fluorescence probe in peroxy acid detection

A fluorescent probe and peroxyacid technology, applied in the field of fluorescent probes, can solve the problems of harsh conditions, low detection accuracy, and immediate detection, etc., and achieve the effect of fluorescence enhancement

Active Publication Date: 2016-08-10
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are few methods for detecting peroxyacid, and there are many methods for detecting peracetic acid, but because peracetic acid often coexists with hydrogen peroxide, the interference of hydrogen peroxide leads to low detection accuracy; some methods have limitations and cannot detect gas phase Peracetic acid; There are also methods that must be tested immediately after collecting samples, and the conditions are harsh

Method used

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  • A fluorescence probe, a preparing method thereof and applications of the fluorescence probe in peroxy acid detection
  • A fluorescence probe, a preparing method thereof and applications of the fluorescence probe in peroxy acid detection
  • A fluorescence probe, a preparing method thereof and applications of the fluorescence probe in peroxy acid detection

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Embodiment 15

[0031] Example 1 Synthesis of 5-dimethylamine-acenaphthenequinone fluorescent probe

[0032] (1) Synthesis of 5-dimethylamine-acenaphthenequinone fluorescent probe

[0033] 1. Synthesis of 5-bromo-acenaphthenequinone:

[0034] Under the protection of nitrogen, take 3g of acenaphthylquinone (16.5mmol) and 5mL of liquid bromine (180mmol), add them into a 50mL single-necked bottle, slowly raise the temperature to 60-70°C under stirring, and react for 8h. The reaction solution was cooled to room temperature, and saturated Na 2 SO 3 solution until all the red bromine is consumed, then filtered and dried to obtain a crude product, which was recrystallized 4-5 times with acetic acid. 3.9 g of pure light yellow 5-bromo-acenaphthenequinone was obtained, and the yield of the compound was 90%.

[0035] 2. Synthesis of 5-dimethylamine-acenaphthenequinone fluorescent probe:

[0036] Under nitrogen protection, take respectively 208mg 5-bromo-acenaphthenequinone (0.8mmol), 65.2mg dimeth...

Embodiment 2

[0041] Example 2 Application of 5-dimethylamine-acenaphthenequinone fluorescent probe in qualitative and quantitative detection of peroxyacids

[0042] (1) Absorption and emission spectra of 5-dimethylamine-acenaphthenequinone fluorescent probe

[0043] Take 0.5mL of phosphate buffer solution (0.1M pH=7.40) and 10μL of 5mM fluorescent probe stock solution into different centrifuge tubes, and then sequentially add 5mM peroxy Acetic acid solution and 50 mM peracetic acid solution with a volume of 1.5, 2, 2.5, 3, 4, 5, 7, 9, 15, and 25 μL were added to the corresponding centrifuge tubes, and finally 4.5 mL of 1,4-dioxo six rings. Add the solution to the cuvette in turn, measure its UV absorbance, set the width of the grating to 5nm, collect the absorption spectrum information at 350-550nm, and then use 430nm to excite, and collect the fluorescence changes at 440-700nm. Measure the UV-Vis absorption spectrum ( Figure 4a ) and emission spectrum ( Figure 4b ). Such as Figur...

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Abstract

The invention relates to a fluorescence probe, a preparing method thereof and applications of the fluorescence probe in peroxy acid detection. The fluorescence probe is 5-dimethylamino acenaphthoquinone having a structure shown as a formula (I). The preparing method includes mixing 5-bromo acenaphthoquinone, dimethylamine hydrochloride, cuprous iodide and potassium hydroxide with N,N-dimethylformamide, heating, reacting for 2-7 h, filtering, drying and separating to obtain a target product. The fluorescence probe is free of fluorescence itself, fluorescence is enhanced after the fluorescence probe reacts with peroxy acid, and the intensity of the fluorescence is in direct proportion to the concentration of the peroxy acid, thus indicating existence of the peroxy acid or quantitatively measuring the concentration of the peroxy acid.

Description

technical field [0001] The invention relates to the field of fluorescent probes, in particular to a fluorescent probe that can be used to selectively detect peroxyacids. Background technique [0002] The epoxidation of olefins is the main application of peroxyacids. For example, in the textile and paper industries as bleaching agents. Due to their strong antibacterial effects, peracetic acid and peroxycitric acid are widely used as disinfectants. Its biggest advantage is that only non-toxic water and acid are left after disinfection, which is more environmentally friendly than other chlorine-containing compounds. Therefore, it is widely used in food and beverage, hospital and agriculture. However, peracetic acid is highly irritating to the skin, respiratory tract and eyes. Therefore, how to accurately detect peracetic acid is particularly important. It has been reported in the literature that the presence of porphyrin-based peroxyacids was found in the urine of CEP pati...

Claims

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Application Information

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IPC IPC(8): C07C225/32C07C221/00C09K11/06G01N21/64
CPCC07C221/00C07C225/32C09K11/06C09K2211/1011G01N21/643
Inventor 夏立新李亚菲宋朋王婧
Owner LIAONING UNIVERSITY
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