Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting sulphuretin and butein with tyrosinase inhibitory activity from wood powder of lacquer tree

A technology of purpurin and thioflavin, applied in the directions of organic chemistry, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as weak utilization of lacquer seeds, and achieve the effects of high activity, simple process operation and easy implementation.

Active Publication Date: 2016-08-10
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the lacquer tree industry mainly focuses on the production and development of natural lacquer coatings, and the utilization of lacquer seeds is also relatively weak. In addition, in the process of growth and utilization of lacquer trees, a large number of branches and stems have not been utilized. In order to expand the scale of the lacquer tree industry Therefore, it is of guiding significance for the development and utilization of sumac resources to carry out the extraction and preparation of compounds that inhibit tyrosinase activity of sumac polyphenols

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting sulphuretin and butein with tyrosinase inhibitory activity from wood powder of lacquer tree
  • Method for extracting sulphuretin and butein with tyrosinase inhibitory activity from wood powder of lacquer tree
  • Method for extracting sulphuretin and butein with tyrosinase inhibitory activity from wood powder of lacquer tree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] first step

[0037] Dry and remove slag from lacquer tree powder, add ethanol aqueous solution with a mass fraction of 50-95% according to the mass volume ratio of 1:10-1:30 (g:mL), and extract under reduced pressure for 2-3 times, each time 1~3h, filter, combine the filtrates, evaporate under reduced pressure at 40~60°C to remove the solvent, and obtain the aqueous crude extract;

[0038] second step

[0039] Add distilled water to the crude extract at a mass ratio of 1:3~1:10 (g:mL), stir to dissolve, add petroleum ether to extract 1~5 times, separate, and evaporate the solvent under reduced pressure at 40~60°C to obtain petroleum ether Extract; the aqueous layer was extracted 1 to 5 times with ethyl acetate, separated, evaporated under reduced pressure at 40 to 60°C to remove the solvent, and dried to obtain the ethyl acetate extract;

[0040] third step

[0041] Adsorb the ethyl acetate extract and the silica gel packing according to the mass ratio of 1:15-50, th...

Embodiment 2

[0047] first step

[0048] Dry the lacquer tree powder to remove slag, add ethyl acetate aqueous solution with a mass fraction of 50-95% at a mass-volume ratio of 1:10-1:30 (g:mL), and extract 2-3 times under reduced pressure and reflux. Filter for 1-3 hours each time, combine the filtrates, and evaporate the solvent under reduced pressure at 40-60°C to obtain a crude aqueous extract;

[0049] second step

[0050] Add distilled water to the crude extract at a mass ratio of 1:3~1:10 (g:mL), stir to dissolve, add petroleum ether to extract 1~5 times, separate, and evaporate the solvent under reduced pressure at 40~60°C to obtain petroleum ether Extract; the aqueous layer was extracted 1 to 5 times with ethyl acetate, separated, evaporated under reduced pressure at 40 to 60°C to remove the solvent, and dried to obtain the ethyl acetate extract;

[0051] third step

[0052] Adsorb the ethyl acetate extract and the silica gel packing according to the mass ratio of 1:15-50, the c...

Embodiment 3

[0058] first step

[0059] Dry the lacquer tree powder to remove slag, add ethyl acetate aqueous solution with a mass fraction of 50-95% at a mass-volume ratio of 1:10-1:30 (g:mL), and extract 2-3 times under reduced pressure and reflux. Filter for 1-3 hours each time, combine the filtrates, and evaporate the solvent under reduced pressure at 40-60°C to obtain a crude aqueous extract;

[0060] second step

[0061] Add distilled water to the crude extract at a mass ratio of 1:3~1:10 (g:mL), stir to dissolve, add petroleum ether to extract 1~5 times, separate, and evaporate the solvent under reduced pressure at 40~60°C to obtain petroleum ether Extract; the aqueous layer was extracted 1 to 5 times with ethyl acetate, separated, evaporated under reduced pressure at 40 to 60°C to remove the solvent, and dried to obtain the ethyl acetate extract;

[0062] third step

[0063] Adsorb the ethyl acetate extract and the silica gel packing according to the mass ratio of 1:15-50, the c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting sulphuretin and butein with inhibitory activity from wood powder of a lacquer tree, belonging to the field of development and application of natural pharmaceutical chemistry and cosmetics. High-content sulphuretin and butein compounds can be rapidly and effectively prepared from the wood powder of the lacquer tree through abstraction, extraction, silica gel column chromatography and low-and-medium pressure chromatography. Sulphuretin and butein monomers prepared in the invention have low-concentration tyrosinase inhibitory activity and pose no toxic and harmful effect to human beings; the tyrosinase inhibition rates of the monomers are greater than 95% when the concentrations of the monomers are 50 [mu]g / mL; preparation process is simple and fast; the monomers are good sources for development of potential natural tyrosinase inhibitors, can be applied to preparation of whitening cosmetics and for prevention and treatment of hyperpigmentation diseases caused by excess melanin in the human body, and have good commercial prospects.

Description

technical field [0001] The invention relates to a method for extracting thiosulfanthin and butacein which have the activity of inhibiting tyrosinase from sumac powder, and belongs to the technical fields of natural medicine and cosmetics. Background technique [0002] Sumac (Rhus verniciflua stokes) is a deciduous tree of the Anacardiaceae genus Toxicodendron. Sumac is an important special-purpose economic forest in my country. Raw lacquer is a natural resin coating and is known as the "king of coatings". It is also an excellent medicinal wood. The leaves, flowers, roots, bark, fruits, dried lacquer and wood core of sumac tree can be used as medicine, which has the effects of relieving cough, eliminating addiction, stimulating menstrual flow, killing insects, and reducing swelling. The polyphenolic compound in the part is the main active substance after urushiol and urushiol, and a large number of studies have shown that it has excellent antioxidant, antitumor, antibacterial a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/83C07D311/32C07D311/40A61K8/49A61Q19/02A61P17/00
CPCA61K8/4973A61K8/498A61Q19/02C07D307/83C07D311/32C07D311/40
Inventor 王成章陈虹霞
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com