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Functional lipidosome for treating brain tumor and preparing method and application thereof

A liposome, brain tumor technology, applied in the field of brain tumor drug research, can solve problems such as tumor recurrence, improve the effect of chemotherapy, enhance the ability to cross the blood-brain barrier, and prevent tumor recurrence

Inactive Publication Date: 2016-08-17
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the theory of cancer stem cells (CSCs), this does not represent the complete elimination of cancer stem cells. If the cancer stem cells continue to proliferate after treatment, the tumor may relapse after remission

Method used

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  • Functional lipidosome for treating brain tumor and preparing method and application thereof
  • Functional lipidosome for treating brain tumor and preparing method and application thereof
  • Functional lipidosome for treating brain tumor and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the preparation of liposome

[0053] 1. Preparation of blank liposomes

[0054] 1. Preparation of blank liposomes

[0055] lecithin, cholesterol, DSPE-PEG2000, DSPE-PEG2000-NH 2 Dissolve in methanol at a molar ratio of 68.5:33.6:3.6:1, remove methanol by rotary evaporation, and form a film in an eggplant-shaped flask by rotary evaporation at 35°C and 40rpm, add 10ml of 250mM ammonium sulfate, ultrasonicate in a water bath for 5min, and then transfer to The serum bottle was further ultrasonicated in the JY92 ultrasonic cell pulverizer (the ultrasonic working time was 10s, the intermittent time was 30s, the whole process time was 10min, and the protection temperature was 35°C), and then the liposome extruder was used to pass through 400nm and 200nm respectively. Polycarbonate membranes were used 3 times each, and the liposomes were replaced with PBS solution (pH=7.4) by dialysis to obtain blank liposomes.

[0056] 2. Preparation of blank liposomes modifie...

Embodiment 2

[0063] Embodiment 2, the performance measurement of liposome

[0064] 1. Determination of MAN modification rate

[0065] Liposome NH 2 -The amino group of PEG2000-DSPE reacts with trinitrobenzene sulfate, and the number of amino groups that can react with trinitrobenzene sulfate in MAN-UA-EGCG-liposome is detected accordingly. The MAN modification rate of MAN-UA-EGCG liposome is: [1-(MAN-UA-EGCG-liposome) can react with trinitrobenzene sulfuric acid amino quantity / blank liposome can react with trinitrobenzene The number of amino groups reacted with phenylsulfuric acid]×100%. The connection efficiency of MAN on the surface of MAN-UA-EGCG-liposome was measured to be 32.95±0.06%.

[0066] 2. Determination of Encapsulation Efficiency

[0067] Sephadex G-50 column chromatography was used to remove ursolic acid not contained in liposomes, and an appropriate amount of UA-EGCG-liposomes and MAN-UA-EGCG-liposomes were digested with methanol before passing through the column Determ...

Embodiment 3

[0082] Example 3. Research on the inhibitory effect of drug-loaded liposomes on C6 glioma cells and C6 glioma stem cells in vitro

[0083] 1. Training conditions

[0084] 1. C6 glioma cell culture conditions

[0085] C6 glioma cells were cultured in DMEM high-glucose medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin at 37 °C, 5% CO 2 Incubated in a saturated humidity incubator, the C6 glioma cells grew adherently, the cell shape was spindle-shaped, the cytoplasm was stretched, and there were protrusions between the cells connected to each other ( image 3 As shown in (A), the cells in the logarithmic growth phase were taken for the experiment.

[0086] 2. C6 glioma stem cell culture conditions

[0087] Take C6 cells in the logarithmic growth phase, wash with PBS, digest with 0.25% trypsin, pipette into single cell suspension, resuspend in serum-free medium (containing DMEM / F12, bFGF, EGF, B27), 4~ After 5 days, the cells proliferate t...

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Abstract

The invention discloses functional lipidosome for treating a brain tumor and a preparing method and application thereof. The target lipidosome is composed of lipidosome and 4-aminophenyl-alpha-D-pyran mannoside; 4-aminophenyl-alpha-D-pyran mannoside is connected to an amino group of the lipidosome, an amino groupd of the lipidosome is provided by distearoyl phosphatidyl ethanolamine-polyethylene glycol-amidogen. The surface of the lipidosome is modified by MAN, the capacity for striding blood brain barrier is enhanced, and the functional lipidosome has the function of targeting tumor cells; the antineoplastic drug ursolic acid and an iagent EGCG for inducing tumor cell apoptosis are encapsulated into the lipidosome to obtain multifunctional target brain tumor stem cell compound lipidosome so that a drug can reach the focus position after striding the BBB, the two drug materials are combined to kill tumor cells and induce tumor cell apoptosis, the chemotherapy effect is greatly improved, and the functional lipidosome is expected to effectively prevent tumor relapse. The functional lipidosome brings hope for thoroughly radical treatment the brain tumor and has important theoretical significance and clinical significance.

Description

technical field [0001] The invention relates to a functional liposome for treating brain tumors, a preparation method and application thereof, and belongs to the field of brain tumor drug research. Background technique [0002] In recent years, the morbidity and mortality of tumors have increased significantly, and the incidence of glioma in adult malignant tumors also accounts for a very high proportion. Glioma has a strong ability to invade, and invasive glioma cells can quickly infiltrate and destroy normal brain tissue. Surgical treatment is difficult to completely remove gliomas, and gliomas have poor sensitivity to radiotherapy and chemotherapy, and the recurrence rate is extremely high, resulting in poor prognosis of gliomas, which seriously threatens human health and life. One of the tumors with the highest mortality rate. Despite treatment with a combination of surgical resection, radiation therapy, and chemotherapy, the average survival time of patients has not c...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/48A61K31/56A61P35/00A61K31/353
CPCA61K31/56A61K31/353A61K2300/00
Inventor 应雪王亚华闫荷露李霞唐辉
Owner SHIHEZI UNIVERSITY
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