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Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof

A technology of arabinose and rhamnoside, applied in the field of extraction and preparation of plant active ingredients, quercetin-3-O-α-L-arabinose--α-L-rhamnoside extraction, can solve the problem of poor yield. High, complex extraction and purification process, not the preparation process of flavonoid glycosides, etc., to achieve the effects of short preparation cycle, wide practical value, and simple preparation process

Active Publication Date: 2016-08-24
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are studies on the detection and identification of flavonoid glycosides in the leaves of C. chinensis, which involve the extraction and purification process, but mainly the steps required for the determination, not the preparation process of flavonoid glycosides, and the extraction and purification process is complicated, and the yield is low. high

Method used

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  • Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof
  • Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof
  • Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Extraction of quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves

[0032] The specific extraction method steps for preparing quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves are as follows:

[0033] (1) Pretreatment of raw materials: Harvest fresh red jujube leaves without insect erosion, wash them with clean water, dry them at 45°C, crush them, and pass them through a 40-60 mesh sieve.

[0034] (2) Extraction and concentration: the pulverized red jujube leaves prepared in step (1) were extracted with 75% ethanol solution, and the ratio of solid to liquid was 1:10, that is, g / ml. After 2 h, filter, combine the filtrates for 4 times, and concentrate the extract under reduced pressure at low temperature to obtain the total flavonoid extract with a yield of 13.0%.

[0035] (3) Elution: Dissolve the total flavonoids extract obtained in step (2) with 4 times the volume of distilled water, and then put it on a macroporous resin ...

Embodiment 2

[0043] Example 2: Preparation of quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves

[0044] The specific extraction method steps for preparing quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves are as follows:

[0045] (1) Pretreatment of raw materials: Harvest fresh red jujube leaves in November without insect erosion, wash with clean water, dry at 45°C, crush, crush and pass through a 40-mesh sieve.

[0046] (2) Extraction and concentration: Weigh 500 g of crushed jujube leaves prepared in step (1) and extract with 5 L of 75% ethanol solution, reflux extraction at 60°C for 2 h each time, filter, and combine for 4 times The filtrate and the extract were concentrated under reduced pressure at low temperature to obtain the total flavonoid extract with a yield of 13.0%.

[0047] (3) Elution: Dissolve the total flavonoid extract obtained in step (2) with 500 ml of water and apply it to a macroporous resin column at a flow rate of 1 ml / min. Afte...

Embodiment 3

[0054] Harvest red jujube leaves, wash them with clean water, dry them at 45°C, grind the dried red jujube leaves with a pulverizer, extract the dried sample powder with 75% ethanol solution, concentrate under reduced pressure at low temperature, and obtain the total flavonoid extract with a yield of 13.0% . The total flavonoid extract was dissolved in an appropriate amount of water and applied to a macroporous resin column, eluted with 20%, 50%, and 95% ethanol solutions respectively, collected 50% of the eluate, and recovered the solvent to obtain crude flavonoid glycosides with a yield of 3.5%. The crude flavonoid glycosides were dissolved in 3 times the volume of 50% methanol and refined and purified by preparative HPLC, the eluent was detected by analytical HPLC, the same fractions with a purity greater than 98% were combined, the solvent was recovered, and quercetin was obtained respectively Quercetin-3-O-bacoside, quercetin-3-O-rutinoside and a rare flavonoid glycoside ...

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Abstract

The invention discloses quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof. The red date leaves are collected, washed clean through clear water, dried, smashed and extracted through a 75% alcohol solution, low-temperature vacuum concentration is carried out, general flavone extract is acquired and dissolved through a proper amount of water, the product is put onto a macroporous resin column, elution is carried out through a 20% alcohol solution, a 50% alcohol solution and a 95% alcohol solution, 50% eluant is collected, a solvent is recovered, crude flavonoid glycoside is obtained and dissolved through 50% methyl alcohol with the volume three times that of the crude flavonoid glycoside, rectification purification is carried out through a preparative efficient liquid phase, the eluant is detected through an analytical type efficient liquid phase, the same flow with purity larger than 98% is mixed, a solvent is recovered, and a flavonoid glycoside quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside monomer is obtained, the product purity and the yield are high, and the monomer can serve as a standard substance for analysis and detection, has an obvious application effect for treating leishmania and has high practical value.

Description

technical field [0001] The invention relates to the technical field of extraction and preparation of plant active ingredients. Specifically, the invention relates to the technical field of extracting quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside for treating Leishmania. Background technique [0002] Jujube is a plant of the genus Ziziphus in the family Rhamnaceae, and its fruit is called jujube or jujube, which is not only a kind of delicious fruit and dried fruit, but also a kind of Chinese medicine with remarkable curative effect. At present, domestic and foreign processing and technical research are limited to jujube fruit, and there are few studies on other resources of jujube tree, such as roots, stems, leaves, flowers and bark. As of 2015, the planting area of ​​jujube trees in the country has reached 17 million mu. Jujube leaves are the largest by-product of jujube tree production. The annual output of fresh jujube leaves per mu of jujube fields is about 200 kilog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 白红进陈朋邹玲周忠波吴翠云
Owner TARIM UNIV
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