Screening and application of marine Schizophyllum strains
A technology of Schizophyllum and Schizophyllin, which is applied in the directions of application, medical preparations containing active ingredients, fungi, etc., to achieve the effect of outstanding moisturizing performance and great application and promotion value
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Embodiment 1
[0019] Example 1: Screening of marine-derived white ginseng strains
[0020] 1. Preparation of culture medium
[0021] Use natural seawater or artificially prepared seawater to prepare improved PDA medium: 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of seawater or artificial seawater, and natural pH. The method is to wash and peel the potatoes first, then weigh 200g of potatoes and cut them into small pieces, add sea water to boil until rotten (boil for 20-30 minutes, and the potato pieces can be pierced by a glass rod), filter through eight layers of gauze, and heat. Then add 1-20g of agar according to the actual experiment needs, continue to heat and mix well, after the agar is dissolved, add glucose, stir evenly, and then make up the water of seawater or artificial seawater to 1000ml after a little cooling, divide into conical flasks, and stopper , bandage, (121°C) sterilization for about 20 minutes, take out and shake well, cool to about 60°C,...
Embodiment 2
[0024] Embodiment 2: Identification of white ginseng bacterial strain
[0025] 1. Extraction of fungal DNA genome
[0026] Get the bacterial strain culture solution that embodiment 1 obtains, precipitate after centrifugation and add appropriate amount of dH 2 O was fully ground and crushed, and TaKaRa's commercial fungal nucleic acid extraction kit was selected for DNA extraction.
[0027] 2. Nucleic acid purity and concentration determination
[0028] After taking a certain amount of DNA extract and diluting it to a certain number of times, measure the OD value at 260, 280 and 320 nm respectively, and calculate the nucleic acid purity by (OD260-OD320) / (OD280-OD320), the natural nucleic acid purity range is 116-210 , generally 118±012 is appropriate; nucleic acid concentration (ng / μL)≈50×(OD260-OD320) / L×D (L is the optical path length in cm, D is the dilution factor), according to the results, the nucleic acid concentration is diluted to a suitable The template concentratio...
Embodiment 3
[0037] Embodiment 3: the production of schizophyllin
[0038] 1. Fermentation of Schizophyllum
[0039] The schizophyllum is inoculated into the fermentation medium at an inoculation ratio of 5-10%, and cultured in a shaker flask at 25-27° C. and 160-180 rpm for 24-36 hours. The formula of the fermentation medium is: glucose 30.0g / L, yeast powder 5.0g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.3g / L.
[0040] 2. Extraction and purification of schizophyllin
[0041] Using the Schizophyllum fermentation culture as material, collect the fermentation broth, centrifuge at 8 000r / min for 20min, and discard the precipitate. Add powdered activated carbon to the supernatant, heat and stir in a water bath for 15 minutes, and filter with suction to obtain a viscous, transparent liquid. The decolorized fermented liquid was deproteinized by the Sevag method. After repeating 5 times, 2.5 times the volume of 95% ethanol was added to the deproteinized fermented liquid. A ...
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