Preparation method of dextran

A technology of dextran and preparation steps, which is applied in the field of preparation of dextran, can solve problems such as direct use, complicated operation, and inability to control molecular weight, and achieve the effects of quality assurance, simple operation, and simplified preparation process

Inactive Publication Date: 2016-08-31
高大元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem mainly solved by the present invention: in the process of preparing dextran in the current traditional microbial fermentation method, the molecular weight formed cannot be regulated during the fermentation process, and finally a dextran product with a huge molecular weight is formed, which cannot be used directly, and subsequent processing is required, so that the operation Complicated, and the dextran produced contains a large amount of chloride, which seriously affects the quality and quality of dextran. It provides a method to combine the cultured Leuconostoc membranosa thallus and Penicillium chrysogenum thallium with alginic acid Sodium is mixed, dropped into calcium chloride to granulate, then filtered, dried to obtain immobilized granules, placed in a fermentation tank with sugarcane solution, and after fermentation, raise the temperature and stir to inactivate, so as to obtain the preparation method of dextran

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] First, add 10g sucrose, 0.2g peptone, 0.2g disodium hydrogen phosphate, 0.03g potassium dihydrogen phosphate, 0.5g yeast extract, and 200mL deionized water to a 500mL petri dish in sequence, and mix them to make medium No. Leuconostoc membranosa; then weigh 0.1 g of Leuconostoc enterococcus enterica and inoculate it into the No. 1 medium above, and culture it with a shaker for 96 hours at 20°C, then centrifuge the culture solution at 5000 r / min for 30 minutes, Discard the supernatant to obtain Leuconostoc enterococcus cells for later use; then weigh 20g of peeled potatoes, cut them into pieces, put them into a beaker, add 400mL of deionized water to the beaker, heat to boiling, and keep warm for 20min , and then filter to get the filtrate, add 2g sucrose and 3g agar successively in the filtrate, mix and make No. 2 medium, cultivate Penicillium chrysogenum; then take 0.1g Penicillium chrysogenum and inoculate it in the above-mentioned No. 2 medium, At 25°C, after shaking...

example 2

[0026] First, add 15g sucrose, 0.3g peptone, 0.3g disodium hydrogen phosphate, 0.04g potassium dihydrogen phosphate, 0.6g yeast extract, and 250mL deionized water to a 500mL petri dish in sequence, and mix them to make medium No. Leuconostoc membranosa; then weighed 0.2g of Leuconostoc mesenteroides and inoculated it into the No. 1 medium above, at 23°C, cultured on a shaking table for 110h, then centrifuged the culture solution at 6000r / min for 35min, Discard the supernatant to obtain Leuconostoc enterococcus cells for later use; then weigh 25g of peeled potatoes, cut them into pieces, put them into a beaker, add 450mL of deionized water to the beaker, heat to boiling, and keep warm for 25min , then filter to get the filtrate, add 2.5g sucrose and 4g agar successively to the filtrate, mix and make No. 2 medium, cultivate Penicillium chrysogenum; then weigh 0.2g Penicillium chrysogenum and inoculate it into the above-mentioned No. 2 medium , at 28°C, after shaking with a shake...

example 3

[0029] First, add 20g sucrose, 0.4g peptone, 0.4g disodium hydrogen phosphate, 0.05g potassium dihydrogen phosphate, 0.7g yeast extract, and 300mL deionized water to a 500mL culture dish in sequence, and mix them to make medium No. Leuconostoc membranosa; then weighed 0.3g of Leuconostoc enterococcus enterica and inoculated it into the No. 1 medium above, at 25°C, cultured on a shaking table for 120h, then centrifuged the culture solution at 7000r / min for 40min, Discard the supernatant to obtain Leuconostoc enterococcus cells and set aside; then weigh 30g of peeled potatoes, cut them into pieces and put them into a beaker, add 500mL of deionized water to the beaker, heat to boiling, and keep warm for 30min , and then filter to get the filtrate, add 3g sucrose and 5g agar successively in the filtrate, mix and make No. 2 medium, cultivate Penicillium chrysogenum; then take 0.3g Penicillium chrysogenum and inoculate it in the above-mentioned No. 2 medium, At 30°C, after shaking w...

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PUM

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Abstract

The invention discloses a preparation method of dextran and belongs to the technical field of preparation of the dextran. The preparation method of the dextran comprises the following steps of: mixing cultured leuconostoc mesenteroides thalli, penicillium chrysogenum thalli and sodium alginate; dropwise adding the mixture into calcium chloride and granulating; then filtering and drying to obtain immobilized particles; putting the immobilized particles into a fermentation tank filled with a sugarcane solution; and after fermenting, raising the temperature and stirring and inactivating so as to prepare the dextran. An embodiment shows that the method disclosed by the invention is novel and unique; the fermentation process is simple and feasible and subsequent processing is not needed; in a preparation process, a lot of chlorides are not generated, the process is simplified and the quality of the dextran is guaranteed; the weight-average molecular weight of the finally prepared dextran is 5000D-7000D, the molecular weight distribution is smaller than 2.0 and the pharmaceutical criteria are completely met.

Description

technical field [0001] The invention discloses a preparation method of dextran, belonging to the technical field of dextran preparation. Background technique [0002] Dextran, also known as dextran, is a polymer formed by the dehydration of several glucose. It is the first microbial polysaccharide discovered in the world and used as the main plasma substitute earlier. Dextran is a polymer formed by the dehydration of several glucans, which is mainly connected by α-1, 6 glycosidic bonds of glucose. [0003] At present, it has been used as a carrier in many drugs, including hypoglycemic drugs, antineoplastic drugs, immune proteins and DNA, etc. In the application of food industry, dextran, as a natural source of rich microbial polysaccharide, is an excellent ingredient of food. It is mainly used as a humectant, stabilizer, extender and thickener in food. In the petroleum industry, dextran is used as an additive for oil well drilling mud. [0004] At present, the synthesis...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/08C12N11/10C12R1/01C12R1/82
CPCC12P39/00C12N11/10C12P19/08
Inventor 高大元张明孟浩影
Owner 高大元
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