Preparation method of bio-functionalized chitosan hydrogel

Abstract

The invention belongs to the field of tissue engineering biological materials, and specifically relates to a preparation method of bio-functionalized chitosan hydrogel. The preparation method comprises the following steps: preparing genipin solutions with different weight percentages: (1) individually dissolving 0.005 gram of genipin, 0.01 gram of genipin, and 0.02 gram of genipin into 1 mL of alcohol (75%) to prepare a genipin solution (0.5%), a genipin solution (1.0 %), and a genipin solution (2.0%); (2) preparing a chitosan solution: dissolving 1.5 grams of chitosan into 100 mL of acetic acid solution (3%) to prepare a chitosan solution (1.5%), adding biological molecules containing amino groups, and stirring the mixture in a bottle for a whole night; (3) mixing two solutions according to different ratios, and allowing the mixed solution to stand still for 24 to 72 hours to obtain the functionalized chitosan hydrogel; wherein the mole ratio of genipin to biological molecules containing amino groups is maintained in a range of 10:5 to 10:1; and the mole ratio of chitosan to genipin is in a range of 300:1 to 30:10. The operation is simple, the cost is low, the reaction conditions are mild and matured, the cytotoxicity is little or none, and the method is universal.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomaterials, in particular to a method for preparing biofunctionalized chitosan hydrogel. Background technique [0002] The chemical name of chitosan is (1,4)-2-amino-2-deoxy-β-D-glucose, which is a natural homogeneous linear polysaccharide containing amino groups. Acetyl products. The final product of chitosan degradation in vivo is CO 2 and glycoproteins, these two substances can be directly excreted from the body or absorbed by the organism. In addition, the free amino groups contained in chitosan greatly enhance its reactivity and functionality. Because chitosan has the advantages of wide sources, good biocompatibility and degradability, chitosan-based hydrogel has become one of the most popular hydrogel materials currently researched, and it has shown great application in the field of biomedicine. value. [0003] As a biomedical material, the mechanical properties of chitosan often fai...

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Problems solved by technology

However, there are still problems to be solved in the traditional functionalization method. For example, (1) the steps are complicated. This method needs to modify the chitosan in advance or modify the synthetic hydrogel. Any chemical modification step may introduce Factors unfavorable to organisms, Kim et al. terminated 2-carboxyethyl acrylate on the primary amino group of chitosan, and then mixed it with PEO hexathiol to obtain chitosan-PEO in situ hydrogel; (2) Reaction Under harsh conditions, special functionalized chitosan blended hydrogels are synthesized by methods such as ultraviolet light or radiation, but for biofunctional materials, such methods can lead to the denaturation of introduced biofunctional molecules (proteins, DNA, etc.) Inactivation; (3) There are many kinds of chemical reagents, and the biological application of chemical reagents to biological functional molecules and chitosan hydrogels will increase cytotoxicity, etc. Kim et al. used 2,2-dimethoxy-2-phenylacetophenone Preparation of Chitosan / Polyacrylamide Interpenetrating Network (IPN) Hydrogel by Reaction of (DM-PAP) and Methylenebisacrylamide (MBAAm) as Initiator and Crosslinker

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