Surface enhanced Raman scattering substrate and manufacturing method thereof

A surface-enhanced Raman and substrate technology, applied in Raman scattering, measuring devices, instruments, etc., can solve problems such as difficulty in forming abundant nano-scale particle gaps, limited SERS enhancement performance, and difficulty in ensuring repeatability, and achieve equipment The effect of low requirements, reduced requirements, and simple and easy process

CN105954251AInactive Publication Date: 2016-09-21NANJING UNIV OF POSTS & TELECOMM
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2016-09-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a surface-enhanced Raman scattering substrate and a preparation method thereof. The substrate is sequentially immersed in the first cleaning solution, the second cleaning solution and the third cleaning solution, and subjected to ultrasonic treatment respectively; solution, 4‑styrene sulfonate sodium aqueous solution, and polydiallyldimethylammonium chloride aqueous solution for modification; the gold nanoparticle sol was co-cultured with the substrate modified by multiple charges, and physical deposition and electrostatic interaction The solid substrate of the SERS active gold nanoparticle film was prepared by the action. The preparation method of the SERS substrate provided by the present invention is simple and easy to implement, has low requirements on equipment, and can realize the preparation of a large-area SERS substrate. The prepared SERS substrate has good performance and has a certain application prospect in biochemical detection.
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Description

technical field

[0001] The invention relates to the field of preparation of Raman scattering substrates, in particular to a surface-enhanced Raman scattering substrate and a preparation method thereof. Background technique

[0002] Surface-enhanced Raman scattering (SERS) has the following three obvious advantages as an analysis and detection method: (1) High sensitivity: the enhancement factor of SERS can reach up to 10 14 -10 15 , enabling single-molecule detection. (2) High selectivity: SERS can only enhance the target molecule or group in a complex system, and obtain the fingerprint spectrum information of the target molecule. (3) Mild detection conditions: SERS spectroscopy can be used in aqueous systems, and the physical form of the sample is not limited.

[0003] In order to use SERS as a routine and on-line analysis tool, the prepared SERS substrate should have the characteristics of strong enhancement ability, good uniformity, easy preparation and storage, and co...

Examples

Embodiment 1

[0033] A surface-enhanced Raman scattering substrate, using physical deposition and electrostatic interaction to assemble a nanoparticle film on a substrate that has been cleaned and surface charge modified, the substrate is a silicon chip, and the material of the film layer is gold nanoparticles .

[0034] The physical deposition method of the surface-enhanced Raman scattering (SERS) substrate includes the following steps:

[0035] In step 1, ethanol with a volume fraction of 99.7% was used as the first cleaning solution, acetone with a volume fraction of 99.5% was used as the second cleaning solution, and ultrapure water with a resistivity of 18.2 MΩ·cm was used as the third cleaning solution.

[0036] Step 2, the preparation mass fraction is 9.8% aminosilylating reagent ethanol solution, mass fraction is 3% 4-styrene sulfonate sodium aqueous solution, mass fraction is 2% polydiallyl dimethyl ammonium chloride For the aqueous solution, the original solution can be diluted t...

Embodiment 2

[0042] Steps 1-5 of this embodiment are the same as in Embodiment 1, and step 7 repeats the operation of step 6, specifically:

[0043] Step 6: Co-cultivate 20 μL of gold nanoparticle colloid with the substrate in a wet box, then put the wet box at 4°C and let it grow for 15 h, remove the gold nanoparticle sol with a liquid gun, and dry it in an oven.

[0044] Step 7, co-cultivate 20 μL of gold nanoparticle colloid with the substrate dried in step 6 in a wet box, then put the wet box at 4°C and let it grow for 15 h, then remove the gold nanoparticle sol with a liquid gun, and place in an oven. Medium-dried surface-enhanced Raman scattering substrates.

Embodiment 3

[0046] Steps 1-6 of this embodiment are the same as in Embodiment 2, and step 7 repeats the operation of step 6 twice.

[0047] Examples 1 to 3 are surface-enhanced Raman scattering substrates prepared by forming a film once, forming a film twice and forming a film three times respectively. The scanning electron microscope photos are as follows: figure 2 shown, from figure 2 It can be seen that the SERS enhancement and gold nanoparticle density can be regulated by different film-forming times. By changing the film-forming times, the density of gold nanoparticles on the substrate surface and the SERS enhancement can be adjusted. In the limited contact area and gold nanoparticle sol Under the premise of this, a single film formation cannot guarantee uniform nanoparticle film formation in all areas, and repeated deposition operations can solve this problem well.

[0048] The SERS spectrum obtained by scanning the Raman molecule 4-mercaptobenzoic acid (4-MBA) on the surface-enh...