Preparation method and applications of targeting prussian blue nano particles

A Prussian blue and nanoparticle technology, applied in the field of preparation of targeted Prussian blue nanoparticles, can solve the problems of high control requirements, difficult industrialization promotion, complex reaction process, etc., and achieves few control links and good photothermal conversion. Efficiency and simple operation

Inactive Publication Date: 2016-09-28
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The surface of the nanoparticles is coated with hyaluronic acid, which not only protects the Prussian blue from the influence of the physiological environment, but also has a lymphatic targeting effect. However, the reaction process of this method is relatively complicated, and the requirements for the control of each link are high, so it is not easy to promote industrialization

Method used

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  • Preparation method and applications of targeting prussian blue nano particles
  • Preparation method and applications of targeting prussian blue nano particles
  • Preparation method and applications of targeting prussian blue nano particles

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0032] Pre-preparation: Weigh 0.378g of hyaluronic acid, add 20ml of water, and divide it into two evenly after dissolution.

[0033] S1. Weigh 0.658g K 3 [Fe(CN) 6 ] Add one part of the above-mentioned dissolved hyaluronic acid aqueous solution, stir to dissolve, and obtain the mixed solution A;

[0034] S2. Weigh 0.784g (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O add another part of the above-mentioned dissolved hyaluronic acid aqueous solution, stir and dissolve to obtain the mixed solution B;

[0035] S3. Slowly add the mixed solution B to the mixed solution A, heat and stir, the heating temperature is 60° C., and react for 5 hours to obtain an aqueous solution containing Prussian blue nanoparticles;

[0036] S4. Add 40 mL of acetone to the prepared blue solution, and centrifuge with a centrifuge to obtain a blue solid A;

[0037] S5. Wash the blue solid A with distilled water, then centrifuge, and repeat 3 to 8 times to obtain the blue solid B;

[0038] S6. Vacuum drying the b...

preparation Embodiment 2

[0040] Pre-preparation: Weigh 0.378g of hyaluronic acid, add 20ml of water, and divide it into two evenly after dissolution.

[0041] S1. Weigh 0.658g K 3 [Fe(CN) 6 ] Add one part of the above-mentioned dissolved hyaluronic acid aqueous solution, stir to dissolve, and obtain the mixed solution A;

[0042] S2. Weigh 0.556g FeSO 4 ·7H 2 O add another part of the above-mentioned dissolved hyaluronic acid aqueous solution, stir and dissolve to obtain the mixed solution B;

[0043] S3. Slowly add the mixed solution B to the mixed solution A, heat and stir, the heating temperature is 60° C., and react for 5 hours to obtain an aqueous solution containing Prussian blue nanoparticles;

[0044] S4. Add 40 mL of acetone to the prepared blue solution, and centrifuge with a centrifuge to obtain a blue solid A;

[0045] S5. Wash the blue solid A with distilled water, then centrifuge, and repeat 3 to 8 times to obtain the blue solid B;

[0046] S6. Vacuum drying the blue solid B to obt...

preparation Embodiment 3

[0048] Pre-preparation: Weigh 0.378g of hyaluronic acid, add 20ml of water, and divide it into two evenly after dissolution.

[0049] S1. Weigh 0.658g K 3 [Fe(CN) 6 ] Add one part of the above-mentioned dissolved hyaluronic acid aqueous solution, stir to dissolve, and obtain the mixed solution A;

[0050] S2. Weigh 0.398g FeCl 2 4H 2 O add another part of the above-mentioned dissolved hyaluronic acid aqueous solution, stir and dissolve to obtain the mixed solution B;

[0051] S3. Slowly add the mixed solution B to the mixed solution A, heat and stir, the heating temperature is 60° C., and react for 5 hours to obtain an aqueous solution containing Prussian blue nanoparticles;

[0052] S4. Add 40 mL of acetone to the prepared blue solution, and centrifuge with a centrifuge to obtain a blue solid A;

[0053] S5. Wash the blue solid A with distilled water, then centrifuge, and repeat 3 to 8 times to obtain the blue solid B;

[0054] S6. Vacuum drying the blue solid B to obta...

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Abstract

The invention discloses a preparation method and applications of targeting prussian blue nano particles. The targeting prussian blue nano particles are synthesized by adopting hyaluronic acid, potassium ferricyanide and ferrous chloride, ammonium ferrous sulfate or ferrous sulfate through a one-step method. The preparation method is easy and convenient to operate, the raw materials are safe and easily available, and the prepared nano particles are small in cytotoxicity and good in biocompatibility, have good dispersibility in aqueous solution, have good absorption in infrared and near-infrared areas, and also have good photothermal conversion efficiency; in addition, the prepared nano particles have good photosensitization, and can generate singlet oxygen under the irradiation of infrared light or near-infrared light. The prepared prussian blue nano particles can be used for the photo-thermal and photodynamic therapy in a targeting manner, and have the broad application prospect in the treatment field of cancer.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method and application of targeted Prussian blue nanoparticles. Background technique [0002] Photothermal therapy (PTT) and photodynamic therapy (PDT), a new therapy for tumor photothermal therapy (Photothermal therapy, PTT), a photothermal conversion agent that converts light energy into heat energy, and the photodynamic agent generates active oxygen under the irradiation of light to make the tumor A new cancer treatment method that kills cancer cells by reaching a certain temperature in the tissue or increasing the level of active oxygen. Due to its unique advantages such as high selectivity and low systemic toxicity, photothermal therapy has become a new cancer treatment method that is expected to replace traditional cancer treatment methods. However, there are still some problems and deficiencies in the research work in this emerging field. The traditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/36A61P35/00
CPCA61K9/5161A61K41/0057
Inventor 蒋邦平沈星灿周波
Owner GUANGXI NORMAL UNIV
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