A kind of conductive silk fibroin material and preparation method thereof

A technology of silk fibroin and conductive thread, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the difficult problems of polymerization modification and achieve the effect of uniform conductive layer structure and firm connection

Active Publication Date: 2018-07-17
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The corresponding monomer of PEDOT-OH, EDOT-OH, is a kind of thiophene, which has a different state of matter compared with pyrrole monomer (EDOT is generally solid, and pyrrole is liquid), which is obviously more hydrophobic than the latter. The former has different binding forces and effects with silk fibroin materials in the water environment. The former polymer is easier to deposit in the solution, and the polymerization modification is more difficult. Not only do other modification additives need to be added, but also the technology has been reported. The single secondary valent bond linking method can not well meet the requirements of the combination of PEDOT-OH and silk fibroin matrix; therefore, at present, the in-situ chemical conduction of regenerated silk fibroin (RSF) materials by PEDOT series conductive polymers The technological method of polymerization modification has not been reported yet

Method used

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  • A kind of conductive silk fibroin material and preparation method thereof
  • A kind of conductive silk fibroin material and preparation method thereof
  • A kind of conductive silk fibroin material and preparation method thereof

Examples

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Embodiment 1

[0043] A preparation method of a conductive silk fibroin material, firstly degumming silkworm cocoons by boiling a sodium carbonate solution with a mass fraction of 0.5%, dissolving them in a 9.0M lithium bromide solution, and concentrating them to a concentration of 15wt%; Then, after the product is obtained by tape casting, it is soaked in 75% ethanol aqueous solution to promote its secondary structure to β-sheet transformation so as to be insoluble in water, that is, to obtain the silk fibroin material through insolubilization treatment;

[0044] Then add it to the aqueous solution of 2-((2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methoxy)acetic acid with a concentration of 20mmol / L Sodium dodecyl sulfate (SDS) with a chemical equivalent of 3 / 10, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) with the same chemical equivalent N-hydroxysuccinimide (NHS), using phosphate buffer solution (pH value is 7.4, the concentration of potassium dihydrogen phosphate is ...

Embodiment 2

[0050] A preparation method of a conductive silk fibroin material, firstly degumming silkworm cocoons by boiling a sodium carbonate solution with a mass fraction of 0.5%, dissolving them in a 9.0M lithium bromide solution, and concentrating them to a concentration of 16wt%; then molded to obtain the product, and then soaked in 76% ethanol aqueous solution to promote its secondary structure to β-sheet transformation so that it is insoluble in water, that is, the silk fibroin block material that has been insolubilized ;

[0051] Then add it to the aqueous solution of 2-((2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methoxy)acetic acid at a concentration of 21mmol / L Sodium dodecyl sulfate (SDS) with a chemical equivalent of 3 / 10, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) with the same chemical equivalent N-hydroxysuccinimide (NHS), using phosphate buffer solution (pH value is 7.4, the concentration of potassium dihydrogen phosphate is 0.038M, the concentratio...

Embodiment 3

[0057] A preparation method of a conductive silk fibroin material, firstly degumming silkworm cocoons by boiling a sodium carbonate solution with a mass fraction of 0.5%, dissolving them in a 9.0M lithium bromide solution, and concentrating them to a concentration of 18wt%; adding glycerol with a mass ratio of 6:100 to silkworm cocoons for small molecule plasticization; and then forming the product according to the molding method, and then soaking in 82% ethanol aqueous solution to promote its secondary structure to β folding Transformation so that it is insoluble in water, that is, the insoluble silk fibroin bulk material is obtained;

[0058] Then it was added to an aqueous solution of 2-((2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methoxy)acetic acid at a concentration of 26mmol / L. Sodium dodecyl sulfate (SDS) with a chemical equivalent of 4 / 10, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) with the same chemical equivalent N-hydroxysuccinimide (NHS), usi...

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Abstract

The invention relates to a conductive silk fibroin material and a preparation method thereof. The preparation method mainly comprises the following steps: (1) insoluble treatment of silk fibroin material; (2) 2-((2,3-dihydrothieno[3,4-b][1,4]dioxene-2-yl)methoxy)acetic acid activation product graft modification of silk fibroin material subjected to insoluble treatment; and (3) in-situ oxidation polymerization of thieno[3,4-b]-1,4-dioxin-2-methanol on silk fibroin material surface subjected to graft modification. The method can be used for preparing the silk fibroin conductive composite material with the polythieno[3,4-b]-1,4-dioxin-2-methanol grafted on the surface; the surface sheet resistance is 0.9*10<5>-5*10<7>ohm; the preparation technique is simple and mild; and the obtained novel biological material has high application value, and especially can be used as a nervous tissue engineering material and a neural restoration material.

Description

technical field [0001] The invention belongs to the technical field of silk fibroin material modification, and relates to a conductive silk fibroin material and a preparation method thereof. Background technique [0002] Silk fibroin is a non-toxic, non-irritating, natural polymer material with good biocompatibility and biodegradability (Gregory H.Altman.Silk-based biomaterials; Biomaterials, 2003,24:401-416. ), which has great application potential in the preparation of tissue engineering materials with nerve stimulation and repair functions. [0003] In recent years, many literatures have reported that electrical stimulation can affect the growth and migration of nerve cells. Therefore, in order to enhance the conductivity of silk fibroin materials, construct micro-electromechanical systems and nerve repair materials with excellent performance that can be applied in vivo, silk There have been many reports on the conductive modification of plain proteins; mainly including ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08H1/00C08G61/12A61L27/22A61L27/50
CPCA61L27/22A61L27/50A61L2430/32C08G61/122C08G61/126C08G2261/128C08G2261/324C08H1/00
Inventor 张耀鹏庄奥朱波邵惠丽胡学超
Owner DONGHUA UNIV
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