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Blood microorganism culture bottle

A microbial culture and blood technology, which is applied in the field of medical testing, can solve the problems of poor accuracy of blood microbial culture, achieve the effects of overcoming the interference of measurement results, avoiding interference, and improving sensitivity

Inactive Publication Date: 2016-10-26
郑州点石生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a blood microorganism culture bottle, which solves the problem of poor accuracy of the existing blood microorganism culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This embodiment provides a blood microorganism culture bottle, which includes a transparent polyester bottle body and a sealed bottle cap. The inside of the plastic bottle is a sterile environment, and the bottom of the bottle body is a polymer semipermeable membrane. The permeable membrane can block the passage of substances with a molecular weight greater than 300. The liquid culture medium is filled in the bottle, and the polymer semipermeable membrane is made of the following components by weight percentage: 35% polyhydroxyethyl acrylate, 4% hydroxypropyl methylcellulose, 5% glycerin, 1.8% polyethylene glycol, 0.05% methylene blue, and water as the balance; the culture solution contains 0.08% activated carbon, 0.04% wheat hull ash, and 0.07% bentonite.

[0020] Methylene blue can produce color changes according to the growth of microorganisms in the bottle.

Embodiment 2

[0022] This embodiment provides a blood microorganism culture bottle, which includes a transparent polyester bottle body and a sealed bottle cap. The inside of the plastic bottle is a sterile environment, and the bottom of the bottle body is a polymer semipermeable membrane. The permeable membrane can block the passage of substances with a molecular weight greater than 300. The liquid culture medium is filled in the bottle, and the polymer semipermeable membrane is made of the following components by weight percentage: 30% polyhydroxyethyl acrylate, 5% hydroxypropyl methylcellulose, Glycerin 3%, polyethylene glycol 2.0%, basic fuchsin 0.01%, and water as the balance; the culture solution contains 0.05% activated carbon, 0.05% wheat hull ash, and 0.05% bentonite.

[0023] The basic fuchsin can produce color changes according to the growth of microorganisms in the bottle.

Embodiment 3

[0025] This embodiment provides a blood microorganism culture bottle, which includes a transparent polyester bottle body and a sealed bottle cap. The inside of the plastic bottle is a sterile environment, and the bottom of the bottle body is a polymer semipermeable membrane. The permeable membrane can block the passage of substances with a molecular weight greater than 300. The liquid culture medium is contained in the bottle, and the polymer semipermeable membrane is made of the following components by weight percentage: 40% polyhydroxyethyl acrylate, 2.5% hydroxypropyl methylcellulose, 6% of glycerin, 1.5% of polyethylene glycol, 0.1% of Evans blue, and the balance is water; the culture solution contains 0.1% of activated carbon, 0.02% of wheat hull ash, and 0.1% of bentonite.

[0026] The Evans blue can produce color changes according to the growth of microorganisms in the bottle.

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Abstract

The invention discloses a blood microorganism culture bottle and belongs to the technical field of medical detection. The culture bottle includes a plastic bottle body and a sealing bottle cover, wherein a sterile environment is formed in the plastic bottle body. The bottom of the bottle body is a high-molecular semi-permeable membrane. The bottle body is filled with a liquid culture medium. The high-molecular semi-permeable membrane is prepared from, by weight percentage ratio, polyhydroxyethyl methacrylate, hydroxypropyl methyl cellulose, glycerol, polyethylene glycol, a fluorescent substance and the balance being water. The liquid culture medium includes active carbon, wheat husk ash and bentonite. The culture bottle is matched with a full-automatic blood culture instrument to achieve full-automatic cultivation and detection of a blood sample. The culture bottle can replace a method of manual blood cultivation, overcomes interferences on a detection result due to other substances in the blood sample and the liquid culture medium in the bottle, has accurate, stable and reliable detection results and hardly has false positive and false negative.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a blood microorganism culture bottle. Background technique [0002] The onset of bacteremia and sepsis is rapid and the mortality rate is high. In the past 20 years, the incidence of blood infection has increased significantly, which does not include a considerable part of bacteremia and sepsis caused by anaerobic infection. Since bacteremia and sepsis are clinically serious life-threatening diseases, the mortality rate is as high as 29%, and blood bacterial culture is an important basis for establishing the diagnosis of bacteremia and sepsis. For patients with severe infectious diseases, is it effective? Cure, to a large extent, depends on timely and accurate bacterial culture reports. At present, except for a few hospitals with good conditions that rely on imported automatic blood culture, most domestic hospitals still conduct blood bacterial culture in a manual way, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/12
CPCC12M23/08C12M23/20C12M23/38C12M29/08
Inventor 李杰
Owner 郑州点石生物技术有限公司
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