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Method for extraction of Channa argus phospholipid by diol silica gel

A technology for phospholipids and glycol bases of snakehead, applied in the field of extracting phospholipids in snakehead, can solve the problems of low processing utilization, low extraction efficiency and low product quality of snakehead, and achieves the effects of stable method, good extraction effect and high phospholipid purity

Active Publication Date: 2016-11-09
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a method for extracting snakehead phospholipids by using diol-based silica gel; the method effectively combines liquid-liquid extraction and solid-phase extraction to extract phospholipids in snakehead with high purity, thereby solving the problem in the prior art. Existing problems such as low extraction efficiency, low product quality, and low degree of processing and utilization of snakehead

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  • Method for extraction of Channa argus phospholipid by diol silica gel
  • Method for extraction of Channa argus phospholipid by diol silica gel

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Embodiment 1

[0053] Embodiment 1, a kind of method that utilizes diol-based silica gel to extract the phospholipid of snakehead, carry out the following steps successively:

[0054] 1), raw material pretreatment:

[0055]Take fresh snakehead, remove the skin, bone, and viscera to obtain muscle tissue, wash it, grind it into mince, dry it in vacuum until the water content is ≤5%, and then pulverize it into powder (passing through a 80-100 mesh sieve) to obtain snakehead powder.

[0056] 2), liquid-liquid extraction:

[0057] Accurately weigh 0.1 g of ground snakehead powder, extract with 3 mL of chloroform-methanol (2:1, volume ratio) mixture in an ice bath for 30 min, add 1.5 mL of ultrapure water and operate at 12,000 rpm / min high-speed refrigerated centrifugation for 5 minutes. Use a 200mL pipette gun to transfer the supernatant, add 2mL chloroform to the remaining supernatant and solids, and repeat the extraction twice according to the above operation. The three supernatants were co...

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Abstract

The invention discloses a method for extraction of Channa argus phospholipid by diol silica gel. The method comprises the steps of: 1) raw material pretreatment: taking fresh Channa argus meat, conducting washing and grinding, then subjecting the ground meat to vacuum drying, and performing crushing so as to obtain Channa argus powder; 2) liquid-liquid extraction: adding an organic solvent I into the Channa argus powder, then fully mixing the substances evenly, conducting ice-bath ultrasonic extraction, then adding water to perform oscillation centrifugation, when the solution stratifies, removing the lower clear liquid; firstly drying the lower clear liquid, then performing redissolving with an organic solvent II to obtain a lipid crude extract, wherein the organic solvent I is a mixed solution of methanol and chloroform in a volume ratio of 1:0.5-2, and the organic solvent II is methanol; and 3) solid phase extraction: activating a diol silica gel solid phase extraction column, loading the crude extract sample, carrying out leaching and elution so as to obtain a purified phospholipid extract.

Description

technical field [0001] The invention relates to the technical field of material separation, in particular to a method for extracting phospholipids from snakeheads. Background technique [0002] Snakehead, commonly known as black fish, is one of the important freshwater economic fishes in my country. It has strong vitality, strong reproductive ability, delicious taste, high nutritional value, rich in protein, fat, amino acid, and calcium, phosphorus, iron and multivitamins necessary for human body. As a medicine, black fish has the functions of dispelling wind and treating malnutrition, nourishing spleen and Qi, nourishing and recuperating, promoting muscle and blood, and promoting wound healing. At present, the domestic snakehead is mainly sold fresh, and the degree of processing and utilization is low, resulting in a waste of resources. In order to increase the industrial added value of snakeheads, snakeheads, as a resource to be fully developed and utilized, have broad p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 沈清戴志远金仁耀冯俊丽薛静陈康
Owner ZHEJIANG GONGSHANG UNIVERSITY
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