Municipal refuse treatment method

A treatment method, the technology of municipal solid waste, applied in the field of municipal solid waste treatment, can solve the problems that are not suitable for the treatment of large-scale municipal solid waste, achieve high application and promotion value, improve capacity and efficiency, and improve the effect of degradation ability

Active Publication Date: 2016-11-09
广东创晟控股集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] CN 103525804 A A biological treatment agent for garbage degradation. The biological treatment agent is combined with Bacillus cereus, Lactobacillus acidophilus, Bacillus stearothermophilus, Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae and a carrier It can efficiently degrade domestic waste, especially kitchen waste, with a degradation rate of 85%. However, this bacterial agent is specifically suitable for kitchen waste and is not suitable for large-scale urban waste treatment.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0023] Cloning of embodiment 1 lipase gene

[0024] Using Pseudomonas DNA as a template, use primer 1 to add the EcoRI restriction site:

[0025] GGATCCatgggtgtgtatgactacaaga, with primer 2 added SpeI restriction site:

[0026] ACTAGTtcaggcgatcacgattccatcagc; PCR amplified. The PCR reaction conditions of the gene were: 94°C for 5min, 94°C for 45s, 54.6°C for 40s, 72°C for 103s, 30 cycles, 70°C for 10min, 4°C for eternity.

[0027] A band of about 1854 bp was recovered by the gel recovery system, and its sequence was shown in SEQ ID NO: 1 by sequencing. Through blast comparison in NCBI, it is found that it is a kind of lipase.

[0028] According to the conventional gene mutation method in this field, the genes were respectively placed in A 83T, G 101Q, E112S, L124V, T 143G, A 165S, N 249S, L 261K, L 268Q, H 291S, M 322T, A 343M, G 385Q, G 401S, K423v, T 505S, Q 522P, G 529N, I 542E, A 561Q, G 569S, Q 570P, A583S or V 591L are subjected to point mutations. The specific embod...

Embodiment 2

[0029] The preparation of embodiment 2 transgenic yeast cells

[0030] The coding sequence obtained in Example 1, the corresponding mutant sequence, and the pScIKP vector were double-digested with restriction endonucleases EcoRI and SpeI, respectively, and the products after the double-digestion were purified, and the two were ligated overnight with T4DNA ligase, and transformed into a plate , to identify recombinants, 57 positive recombinants were obtained through PCR identification. Among them, the recombinant plasmid pScIKP-Pslip was successfully obtained through plasmid extraction and identification. At the same time, similar recombinant plasmids pScIKP-Pslip-A 83T, pScIKP-Pslip-G 101Q, pScIKP-Pslip-E 112S, pScIKP-Pslip-L 124V, pScIKP-Pslip-T143G, pScIKP-Pslip-A 165S, pScIKP-Pslip-N 249S, pScIKP-Pslip-L 261K, pScIKP-Pslip-L 268Q, pScIKP-Pslip-H 291S, pScIKP-Pslip-M 322T, pScIKP-Pslip-A 343M, pScIKP-Pslip-G 385Q, pScIKP -Pslip-G 401S, pScIKP-Pslip-K 423v, pScIKP-Pslip-T50...

Embodiment 3

[0034] The preparation of embodiment 3 bacterial agents

[0035] Alkaline xylosoxidans denitrifying subspecies CGMCC 1.768, Bacillus amyloliquefaciens CGMCC 1.1177, Debaryomyces hansenii var. Nocardia pasteuri CGMCC 4.1128, each bacteria was expanded and cultivated according to the optimal medium, and the volume ratio of the mixed bacteria solution was 10:5:10:1:5:10 to prepare the corresponding bacterial agent. The concentration of each bacteria before mixing is 2*108 / ml.

[0036] The lipase gene or its variants were introduced into Debaria hansenii var. hansenii CGMCC 2.1831 and Issa rhomboides CGMCC 2.1592 respectively to replace the original Debariea hansenii var. hansenii CGMCC 2.1831 without lipase gene , Issahia rhizoma rhomboides CGMCC 2.1592 corresponding to the preparation to become the corresponding bacterial agent. Bacteria named: Pslip Bacteria, A83T Bacteria, G101Q Bacteria, E112S Bacteria, L124V Bacteria, T143G Bacteria, A165S Bacteria, N249S Bacteria, L261K B...

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Abstract

The invention provides a municipal refuse treatment method. As the high grease decomposing capability, lipase can provide nutrient substances for various microorganisms. When alcaligenes xylosoxidans subsp.denitrificans, bacillus amyloliquefaciens, depubyomyces hansenii hansenula polymorpha variants, diamond issatchenkia, keratinophilic aleurisma carnis and Pasteur nocardia are used in combination, nutrient substances can be fully and reasonably utilized; especially, after lipase is transferred into yeast, the decomposition capability on grease is greatly improved, and thus the capability and efficiency of treating municipal refuse are greatly improved; besides, the whole treatment cycle is shortened, and high application and popularization value is achieved.

Description

technical field [0001] The invention relates to a method for treating urban garbage. Background technique [0002] Although my country is a developing country, the level of urbanization has also increased year by year in recent years. In 2015, my country's urbanization rate reached 56.1%, and the urban permanent population reached 770 million. With such a large population, the amount of garbage generated every day is also huge. Municipal waste is complex and difficult to deal with. Incineration is an important means of municipal waste (including domestic waste and medical waste) disposal. [0003] As one of the main waste treatment technologies at present, waste incineration has a development history of about 150 years. It was first developed in Europe. The heat generated by the treated waste incineration can be used for thermal power generation. Although my country's waste incineration power generation has started Late, but developing rapidly. In 1988, Shenzhen established...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/19C12N1/14C12N9/20B09B3/00C12R1/01C12R1/07C12R1/645C12R1/365
CPCB09B3/00C12N1/14C12N1/20C12N9/20C12Y301/01003Y02W30/78Y02A40/20
Inventor 窦欣童
Owner 广东创晟控股集团有限公司
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