Gene-carrying compound for multiple targeting modification, preparation method and application

A technology of targeted modification and compound, applied in other methods of inserting foreign genetic materials, gene therapy, medical preparations of non-active ingredients, etc., can solve the problem of less research
CN106086079BInactive Publication Date: 2019-11-15TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2019-11-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a multi-targeted modified gene-loaded compound, a preparation method and application. The method comprises the following steps: preparing a membrane targeted peptide modified polycation gene vector (I); evenly mixing a TAT-NLS polypeptide aqueous solution and a nucleic acid aqueous solution to obtain mixed liquor, and preparing the gene vector (I) into suspension liquid; and (4) evenly mixing the suspension liquid and the mixed liquor, and standing to obtain the multi-targeted modified gene-loaded compound. The multi-targeted modified gene-loaded compound is specifically recognized by REDV peptide in membrane targeted peptide and an integrin receptor on the surface of an endothelial cell, and uptake of the cell to the compound is improved. The compound which enters the cell is positioned in a lysosome / endosome structure, polyethyleneimine and TAT jointly act, the escaping capacity of the endosome of the compound is improved, and a target gene is promoted to enter cytoplasm. Through interaction of a nuclear localization signal NLS and a nuclear membrane, nuclear internalization of the target gene is promoted. Transfection efficiency of the target gene in the endothelial cells is high.
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Description

technical field

[0001] The invention relates to a multi-target modified gene-carrying complex, a preparation method and an application thereof, and belongs to the technical field of gene carriers with biological target recognition functions. Background technique

[0002] At present, cardiovascular diseases are becoming more and more prevalent. Cardiovascular disease is one of the leading causes of death worldwide due to poor clinical treatment effects and huge treatment costs. Gene therapy is a promising approach for the treatment of congenital and acquired cardiovascular diseases. With the identification of pathophysiological molecular pathways of heart failure and related cardiovascular diseases, pre-clinical trials of gene therapy have been launched in small and large animal models, and gradually moved to the clinic. However, the initial clinical effects did not meet the expected goals . Viral vectors are still controversial due to their inherent lethal immunogenicity,...

Claims

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