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Porcine circovirus 2-type lipidosome diluent lyophilized product and preparation method thereof

A technology for porcine circovirus and freeze-dried products, which is applied in the field of porcine circovirus type 2 liposome diluent freeze-dried products and its preparation, and can solve the problems of uncertain ratio of inoculated living organisms, differences in semi-finished products, and low virus content , to achieve the effect of enhancing vaccine immunogenicity, reducing batch-to-batch variation, and increasing virus content

Inactive Publication Date: 2016-11-16
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] One of the most important indicators for the quality assessment of veterinary biological products is the batch-to-batch stability. However, it is difficult to control the batch-to-batch stability of semi-finished products in the actual production process. The main reason is that the proportion of live organisms inoculated is uncertain , that is to say, the instability of the inoculation amount leads to large differences in the production of semi-finished products
The aim of the present invention is to solve the problems of large batch-to-batch differences and low virus content in the process of porcine circovirus multiplication

Method used

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  • Porcine circovirus 2-type lipidosome diluent lyophilized product and preparation method thereof
  • Porcine circovirus 2-type lipidosome diluent lyophilized product and preparation method thereof
  • Porcine circovirus 2-type lipidosome diluent lyophilized product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of PBS solution

[0020] Take 6-10g of sodium chloride, 0.05-0.5g of potassium chloride, 1-1.2g of disodium hydrogen phosphate, 0.05-0.5g of potassium dihydrogen phosphate, 0.05-0.2g of calcium chloride, 0.05g of magnesium chloride containing 6 crystal water -0.2g; After mixing the above ingredients, dissolve in 1000ml of double-distilled water, and sterilize under high pressure at 116°C for 30 minutes.

Embodiment 2

[0021] Embodiment 2: the preparation of liposome

[0022] Weigh 6g of soybean lecithin and 2g of cholesterol, dissolve them in 500ml of chloroform, and mix them evenly. Rotate the solution under reduced pressure at 37°C to remove the organic solvent and form a phospholipid film; The PBS solution (prepared in Example 1) was hydrated by rotating in a water bath, and the resulting suspension was subjected to ultrasonic treatment, and finally dehydrated with a homogenizer to prepare liposomes.

Embodiment 3

[0023] Embodiment 3: the preparation of liposome

[0024] Weigh 4g of soybean lecithin and 4g of cholesterol, dissolve them in 500ml of chloroform, and mix them evenly. Rotate the solution under reduced pressure at 37°C to remove the organic solvent and form a phospholipid film; add 200ml of pH 7.0 to the film The PBS solution (prepared in Example 1) was hydrated by rotating in a water bath, and the resulting suspension was subjected to ultrasonic treatment, and finally dehydrated with a homogenizer to prepare liposomes.

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Abstract

The invention discloses a porcine circovirus 2-type lipidosome diluent lyophilized product and a preparation method thereof, and belongs to the technical field of animal biological products. Each 1,000 ml of PBS solution is prepared from 5 g to 10 g of cordyceps polysaccharide, 5 g to 10 g of lycopene, 1 g to 5 g of ginsenoside Rh2, 6 g to 12 g of lipidosome, 10 g to 15 g of saccharose and 10 g to 15 g of mannitol. According to the prepared lipidosome diluent lyophilized product, the difference between batches of semi-finished products can be reduced. The prepared lipidosome diluent lyophilized product can promote reproduction of porcine circovirus 2 type, the virus content can be increased, and vaccine immunogenicity can be enhanced.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a freeze-dried product of porcine circovirus type 2 liposome diluent and a preparation method thereof. Background technique [0002] Porcine circovirus disease is a new infectious disease of pigs caused by porcine circovirus type 2 (PCV2). In addition, the disease can also lead to severe immunosuppression in pig herds, which can easily lead to secondary infection or other concurrent infections. The disease has caused huge economic losses to the pig industry all over the world. It is another important disease newly discovered after porcine reproductive and respiratory syndrome. Vaccination is one of the main measures to prevent PCV-2 infection. [0003] Liposome is a phospholipid bilayer structure carrier with similar properties to biological membranes, and the lipid system of traditional Chinese medicine refers to a kind of ultra-micro spherical drug made ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K9/127A61K47/36A61K47/28A61K47/22A61K39/12A61P31/20
CPCA61K9/19A61K9/127A61K39/12A61K47/22A61K47/28A61K47/36C12N2750/10034
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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