Injectable DBM (decalcified bone matrix) hydrogel and preparation method thereof

Abstract

The invention discloses injectable DBM (decalcified bone matrix) hydrogel and a preparation method thereof and relates to a biological tissue engineering technology. According to the preparation method, homogeneous or heterogeneous bone tissue is degreased, decalcified and decellularized, a freeze-dried DBM is prepared, the freeze-dried DBM is digested with pepsin and is cured under the conditions of neutral pH, 37 DEG C and appropriate salt concentration, and the injectable DBM hydrogel is obtained. The DBM hydrogel prepared with the method has good biodegradability, but most importantly, the DBM hydrogel has an injectable property; meanwhile, by means of decellularization processing, the immunogenicity is greatly reduced, the biocompatibility is better, and the biological rejection reaction is reduced; the injectable DBM hydrogel has the advantages of adopting available materials and being convenient to operate and low in cost, can be applied to various bone defect filling and bone graft fusion of min-invasive orthopedic surgery, and has good clinical application prospect.

Description

technical field [0001] The invention relates to biological tissue engineering technology, in particular to an injectable decalcified bone matrix hydrogel and a preparation method thereof. Background technique [0002] Bone nonunion and large area bone defect caused by trauma, infection, tumor, etc. have always been a difficult problem in orthopedic clinic. At present, the commonly used treatment methods for clinical bone defects are autologous bone, allogeneic bone and artificial bone transplantation. The low amount of autologous bone and the complications of bone harvesting make it difficult to meet clinical requirements; the problem of immune rejection of allogeneic bone is still difficult to completely solve; and the artificial synthetic bone graft materials are poor in osteoinduction and bone proliferation, so finding ideal bone graft materials has become the focus of scholars' research. [0003] Today's clinical surgical operations are showing a trend of minimally inva...

AI Technical Summary

Problems solved by technology

The lack of autologous bone and the complications of bone harvesting make it difficult to meet the clinical needs; the problem of immune rejection of allogeneic bone is still difficult to completely solve; and the artificial synthetic bone graft materials are poor in osteoinduction and bone proliferation, and the search for ideal bone graft materials has become the focus of scholars' research

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