Injectable DBM (decalcified bone matrix) hydrogel and preparation method thereof

A technology of demineralized bone matrix and hydrogel, which is used in pharmaceutical formulations, medical science, tissue regeneration, etc., can solve problems such as poor bone proliferation, and achieve the effects of reduced biological rejection, simple material collection, and convenient operation.

Inactive Publication Date: 2016-12-07
TIANJIN HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The lack of autologous bone and the complications of bone harvesting make it difficult to meet the clinical needs; the problem of immune rejection of allogeneic bone is still difficult to completel

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  • Injectable DBM (decalcified bone matrix) hydrogel and preparation method thereof
  • Injectable DBM (decalcified bone matrix) hydrogel and preparation method thereof
  • Injectable DBM (decalcified bone matrix) hydrogel and preparation method thereof

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Embodiment Construction

[0041] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0042] A kind of preparation method of injectable decalcified bone matrix hydrogel of the present invention is as follows:

[0043] a Prepare freeze-dried sponge containing decalcified bone matrix (the following operations are all at 0-4°C)

[0044] Take fresh porcine cancellous bone, ① Soak the cancellous bone with pH 7.4, 0.01mol / L Tris-HCl buffer solution (containing 0.035 mmol / L phenylmethylsulfonyl fluoride), and grind it with a pulverizer (BILON, model DS) -1) Make cancellous bone into 5mm×5mm×5mm granules, and rinse with distilled water repeatedly;

[0045] ② Use chloroform: methanol = 1:1 mixture to degrease for 12-72 hours, and rinse with distilled water repeatedly;

[0046] ③ Use 0.01mol / L HCl solution (25-30ml / g bone) to decalcify for 12-72 hours, and rinse with distilled water repeatedly;

[0047] ④ Use 2mol / L CaCl 2 Stir for 1h, rinse w...

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Abstract

The invention discloses injectable DBM (decalcified bone matrix) hydrogel and a preparation method thereof and relates to a biological tissue engineering technology. According to the preparation method, homogeneous or heterogeneous bone tissue is degreased, decalcified and decellularized, a freeze-dried DBM is prepared, the freeze-dried DBM is digested with pepsin and is cured under the conditions of neutral pH, 37 DEG C and appropriate salt concentration, and the injectable DBM hydrogel is obtained. The DBM hydrogel prepared with the method has good biodegradability, but most importantly, the DBM hydrogel has an injectable property; meanwhile, by means of decellularization processing, the immunogenicity is greatly reduced, the biocompatibility is better, and the biological rejection reaction is reduced; the injectable DBM hydrogel has the advantages of adopting available materials and being convenient to operate and low in cost, can be applied to various bone defect filling and bone graft fusion of min-invasive orthopedic surgery, and has good clinical application prospect.

Description

technical field [0001] The invention relates to biological tissue engineering technology, in particular to an injectable decalcified bone matrix hydrogel and a preparation method thereof. Background technique [0002] Bone nonunion and large area bone defect caused by trauma, infection, tumor, etc. have always been a difficult problem in orthopedic clinic. At present, the commonly used treatment methods for clinical bone defects are autologous bone, allogeneic bone and artificial bone transplantation. The low amount of autologous bone and the complications of bone harvesting make it difficult to meet clinical requirements; the problem of immune rejection of allogeneic bone is still difficult to completely solve; and the artificial synthetic bone graft materials are poor in osteoinduction and bone proliferation, so finding ideal bone graft materials has become the focus of scholars' research. [0003] Today's clinical surgical operations are showing a trend of minimally inva...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/52
CPCA61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/52A61L2400/06A61L2430/02
Inventor 杨强黄洪超马信龙徐宝山赵艳红杨春梅王连永
Owner TIANJIN HOSPITAL
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