Preparation method for microalgae biodiesel

A technology of microalgae and biodiesel, which is applied in the fields of biofuel, petroleum industry, and fat production, can solve the problems of low biodiesel yield, low catalytic efficiency, long reaction time, etc., and achieves strong environmental adaptability and improved catalytic efficiency. , the effect of large reaction interface

Inactive Publication Date: 2016-12-07
南宁华侨投资区政孙贸易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing biodiesel production process generally has the following problems: low catalytic efficiency, low biodiesel yield, long reaction time, corrosion equipment, complex process, high energy consumption, high cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Monoenterobacteria culture medium: Glucose 20mg / L, NaNO 3 2.4mg / L, KH 2 PO 4 1.5mg / L, chitosan 0.5mg / L, biogas slurry 5mg / L, f2 vitamin 3mL and seawater 1L;

[0027] A preparation method of microalgae biodiesel, comprising the following steps:

[0028] S1. Single intestinal algae culture: Use single intestinal algae as raw material, inoculate it into the culture medium at an inoculation density of 7 million cells / mL, and irradiate it with red and blue LED supplementary light for 12h / day, and the red light in the LED supplementary light The ratio with the blue light is 1:1, the light intensity is controlled at 3500LX, and the culture temperature is controlled at 25°C. After 11 days of cultivation, the density of Monoenterobacterium reaches 80 million cells / mL, and the cells of Monoenterobacteria are collected;

[0029] S2. Grease extraction: After concentrating and drying the cells of the monocytogenes collected in step S1, ultrasonic waves are used to...

Embodiment 2

[0034] Preparation of Monoenterobacteria culture medium: Glucose 25mg / L, NaNO 3 2.0mg / L, KH 2 PO 4 1.7mg / L, chitosan 0.6mg / L, biogas slurry 7mg / L, f2 vitamin 4mL and seawater 1L;

[0035] A preparation method of microalgae biodiesel, comprising the following steps:

[0036] S1. Cultivation of Monocoliform algae: Use Monocoliform algae as raw material, inoculate it into the culture medium at an inoculation density of 5 million cells / mL, and irradiate it with red and blue LED supplementary light for 14h / day, and the red light in the LED supplementary light The ratio with blue light is 2:1, the light intensity is controlled at 3000LX, the culture temperature is controlled at 26°C, cultivated for 10 days, and the density of Monoenterobacteria reaches 80 million cells / mL, and the cells of Monoenterobacteria are collected;

[0037] S2. Grease extraction: After concentrating and drying the cells of the monocytogenes collected in step S1, ultrasonic waves are used to break the wall ...

Embodiment 3

[0042] Preparation of Monoenterobacteria culture medium: Glucose 30mg / L, NaNO 3 1.8mg / L, KH 2 PO 4 2.0mg / L, chitosan 0.7mg / L, biogas slurry 8mg / L, f2 vitamin 3mL and seawater 1L;

[0043] A preparation method of microalgae biodiesel, comprising the following steps:

[0044] S1. Cultivation of Monogutella: Use Monogutella as raw material, inoculate it into the culture medium at an inoculation density of 6 million cells / mL, and irradiate it with red and blue LED supplementary light for 16 hours / day, and the red light in the LED supplementary light The ratio with the blue light is 3:1, the light intensity is controlled at 2500LX, and the culture temperature is controlled at 28°C. After 11 days of cultivation, the density of Monoenterobacteria reaches 80 million cells / mL, and the cells of Monoenterobacteria are collected;

[0045] S2. Grease extraction: After concentrating and drying the cells of S. enterica collected in step S1, ultrasonic waves are used to break the wall of S...

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Abstract

The invention discloses a preparation method for microalgae biodiesel. The preparation method comprises the following steps: inoculating monallanthus salina into a culture medium, performing irradiation for 10-15h / day by virtue of a red-blue bicolor LED light supplement lamp, performing culture for 10 to 11 days at 25 to 28 DEG C, and collecting monallanthus salina cells; performing ultrasonic wall breaking treatment on the collected monallanthus salina cells, performing extraction to obtain monallanthus salina oil by virtue of an organic solvent, adding the monallanthus salina oil, methanol and a zeolite molecular sieve catalyst into a reactor according to a mass ratio of 1:(4-6):(0.01-0.03), performing esterification reaction to obtain an esterification product, and separating and purifying the esterification product to obtain the biodiesel. According to the method, the monallanthus salina which is short in production period, wide in distribution, strong in photosynthesis and high in oil content is taken as a raw material, so that problems of a plant oil raw material during biodiesel development are solved; in addition, the method also has the advantages of simple preparation process, high catalysis efficiency, high oil extraction rate, high product yield and the like, and a good foundation is laid for industrialization and large-scale production of the biodiesel.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, in particular to a method for preparing microalgae biodiesel. Background technique [0002] With the increasing depletion of fossil fuels and the sharp rise in oil prices, the search for renewable energy has become a solution to human survival and to maintain the sustainable development of the world economy. As a new alternative energy, biodiesel is developing rapidly in countries all over the world. Biodiesel refers to oil crops such as soybeans, rapeseed, cotton, palm, etc., wild oil plants and engineering microalgae and other aquatic vegetable oils, animal fats, and catering waste oils, etc. Renewable diesel fuel to replace petrodiesel. The advantages of biodiesel are not limited to renewability. Compared with existing diesel, it also has the following advantages: high cetane number, which makes the combustion performance better; better wetting performance, which makes the fuel injecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C11C3/10C10L1/02
CPCC10L1/02C11B1/04C11B1/10C11C3/10Y02E50/10
Inventor 黄健峻其他发明人请求不公开姓名
Owner 南宁华侨投资区政孙贸易有限公司
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