Method for extracting Lithocarpus polystachyus (Wall.) Rehd phlorizin

A technology of the roots and phloridizins of Rhizoma polycarpae, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low yield of phloridizin and phloridizin yield Few, in-depth analysis and other issues to achieve the effect of good market prospects, low prices, good economic benefits

Active Publication Date: 2016-12-14
GUANGXI GANLIANGJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosan is also widely used in the extraction process of Chinese herbal medicine, but its purification effect is poor
Macroporous resin has the advantages of stable physical and chemical properties, good selectivity, easy regeneration, mild desorption conditions, large adsorption capacity, long service life, easy formation of closed loop, high mechanical strength, and low cost. It is used in the extraction and separation of flavonoids. It is widely used, but in the specific resin type and process, it needs to be further analyzed
[0005] Due to its good curative effect on diabetes and super high sweetness, phlorizin has good application prospects in the pharmaceutical and food industries, but the output of phloridzin is very small, so it is very expensive
The yield of extracting phlorizin in the existing methods is low, and the process is complicated and the cost is high. Therefore, how to improve the yield and purity of phlorizin, optimize the process and reduce the cost is a research hotspot today.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of method for extracting rutizin, comprising the following processing steps:

[0032] (1) Drying: Vacuum freeze-drying the fresh leaves (with a phloridin content of 7.89%) of the fresh leaves of Shikeia polycarpa to a moisture content of ≤15% to obtain dried leaves;

[0033] (2) Pulverization: Put the dried leaves in a traditional Chinese medicine pulverizer, pulverize them to a particle size of 100 mesh, and get Duosui Shike powder;

[0034] (3) Ultrasonic extraction: Take 1 kg of Duosuishike powder and add it to an ultrasonic container, then add ethanol (85% ethanol concentration) and 10 L of dimethyl carbonate mixed solvent with a volume ratio of 3:1, and ultrasonicate at a power of 250W Extract for 30 minutes, place the liquid obtained by ultrasonic extraction in a high-speed centrifuge at 10,000 rpm for 2 minutes to obtain supernatant and precipitate;

[0035] (4) Repeat ultrasonic extraction: put the precipitate obtained in step (3) into an ultrasonic cont...

Embodiment 2

[0042] A kind of method for extracting rutizin, comprising the following processing steps:

[0043] (1) Drying: Vacuum freeze-drying the fresh leaves (with a phloridin content of 7.89%) of the fresh leaves of Shikeia polycarpa to a moisture content of ≤15% to obtain dried leaves;

[0044] (2) Pulverization: put the dried leaves in a traditional Chinese medicine pulverizer, pulverize them to a particle size of 150 mesh, and obtain Duosui Shike powder;

[0045](3) Ultrasonic extraction: Take 1 kg of Duosuishike powder and add it to an ultrasonic container, then add ethanol (ethanol concentration: 90%) and 12L of dimethyl carbonate mixed solvent with a volume ratio of 4:1, and ultrasonically at a power of 300W Extract for 25 minutes, place the liquid obtained by ultrasonic extraction in a high-speed centrifuge at 10,000 rpm for 3 minutes to obtain supernatant and sediment;

[0046] (4) Repeat ultrasonic extraction: Add the precipitate obtained in step (3) to 10L of ethanol and d...

Embodiment 3

[0053] A kind of method for extracting rutizin, comprising the following processing steps:

[0054] (1) Drying: Vacuum freeze-drying the fresh leaves (with a phloridin content of 7.89%) of the fresh leaves of Shikeia polycarpa to a moisture content of ≤15% to obtain dried leaves;

[0055] (2) Pulverization: Put the dried leaves in a traditional Chinese medicine pulverizer, pulverize them to a particle size of 200 mesh, and get Duosuishike powder;

[0056] (3) Ultrasonic extraction: Take 1kg of Duosuishike powder and add it to an ultrasonic container, then add ethanol (ethanol concentration: 95%) and 15L of dimethyl carbonate mixed solvent with a volume ratio of 5:1, and ultrasonicate at a power of 350W Extract for 20 minutes, place the liquid obtained by ultrasonic extraction in a high-speed centrifuge at 10,000 rpm for 5 minutes to obtain supernatant and precipitate;

[0057] (4) Repeat ultrasonic extraction: Add the precipitate obtained in step (3) into an ultrasonic contai...

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Abstract

The invention discloses a method for extracting Lithocarpus polystachyus (Wall.) Rehd phlorizin. The method comprises the following steps: carrying out freeze drying on the fresh leaf of the g Lithocarpus polystachyus (Wall.) Rehd in vacuum, and then smashing to obtain the g Lithocarpus polystachyus (Wall.) Rehd powder; adding ethanol and dimethyl carbonate mixing solvent in the powder to perform ultrasonic extraction, centrifugally separating the extracted liquid to obtain supernatant and precipitate; repeatedly ultrasonically extracting the precipitate to respectively obtain the supernatant, and combining the supernatants; and filtering the supernatant through a Millipore filtration, adsorbing through macroporous resin, and finally reducing pressure and evaporating to dryness to obtain the phlorizin. The yield of the Lithocarpus polystachyus (Wall.) Rehd phlorizin extracted through the method disclosed by the invention reaches 90% or more, and the purity reaches 99.8%. The method disclosed by the method has the advantages of simple process, low cost, low energy consumption and simple operation, the industrial production is easy to realize, and the good economic benefit and the good market prospect are obtained.

Description

technical field [0001] The invention relates to the technical field of active ingredients of natural plants, in particular to a method for extracting radishes radicisin. Background technique [0002] Phlorizin is the glucoside of phloretin, which belongs to dihydrochalcones in flavonoids. Dihydrochalcones are rarely distributed in nature and are called "minority flavonoids". Phlorizin can competitively inhibit the transportation of glucose molecules by glucose transporters, reduce insulin resistance, increase high-density lipoprotein content, and reduce low-density lipoprotein content, so it can significantly reduce fasting blood sugar and improve lipid metabolism. In addition, phlorizin has a very high sweetness, which is 300 times that of sucrose, and its calories are only 1 / 300 of sucrose, so it can be directly added to food, so it has broad application prospects in the development of new drugs and natural health foods . Phlorizin was first discovered in the Rosaceae M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 周福才邢朝斌赵东升林任宁何春蓉李贺
Owner GUANGXI GANLIANGJIAN BIOTECH
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