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Plant essential oil with antibacterial effect as well as preparation method and application thereof

A technology for plant essential oils and plant raw materials, applied in the directions of essential oils/spices, fat production, etc., can solve the problems of backward separation and extraction technology, complicated separation and drying operations, low oil yield by pressing method, etc., to shorten the extraction time and simplify the process. Easy to operate, improve extraction efficiency and extraction yield

Inactive Publication Date: 2016-12-14
北京中农弘科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, the separation and extraction technology of plant essential oils in my country is relatively backward, and most of them use traditional pressing, steam distillation or organic solvent extraction. Steam distillation is currently the most widely used method, but due to the high operating temperature It is easy to cause the thermal decomposition of the heat-sensitive compounds and the hydrolysis of the easily hydrolyzed components, and the pressing method has the disadvantages of low oil yield and short storage time of the finished product. Although the organic solvent extraction method has a high yield of essential oil, there are organic solvent residues, toxic, Shortcomings of long extraction time and complicated subsequent separation and drying operations
Moreover, the current lack of corresponding management and monitoring in our country has resulted in low content of effective essential oils and poor quality stability in forage plant essential oil products, and there have been many problems with the effectiveness and safety of use, which urgently needs to further improve the method of extracting and preparing plant essential oils , and improve the functionality of plant essential oils

Method used

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  • Plant essential oil with antibacterial effect as well as preparation method and application thereof
  • Plant essential oil with antibacterial effect as well as preparation method and application thereof
  • Plant essential oil with antibacterial effect as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Embodiment 1, the antibacterial effect comparison of different plant raw material essential oil extracts

[0093] 1) Clove, lemongrass, oregano, bay leaf, cinnamon, cumin, thyme and other plant materials rich in phenolic plant essential oils are respectively crushed and passed through a 50-mesh sieve. Weigh 100g of the raw material powder into a 2000mL round-bottomed flask, add 800mL of water, that is, make the material-liquid ratio of the crushed plant material to water 1g:8mL, and soak for 1h. Then place the round-bottomed flask on the heating mantle and heat it slowly until slightly boiled, steam distilled for 6 hours, collect the distilled liquid into the water separator through the condenser tube, and release the essential oil in the upper layer through the cock of the water separator to obtain the plant essential oil extract .

[0094] 2) Dilute the plant essential oil extract obtained in step 1) to 1:1, 1:2, 1:4, 1:8, 1:16, 1:32 with sterile water (95% ethanol s...

Embodiment 2

[0099] Embodiment 2, the influence of enzymolysis condition on plant essential oil extraction yield

[0100] 1) According to Example 1, select the top three plants with the antibacterial effect obtained, use crushed cinnamon, oregano, and thyme to form the source of raw materials at a ratio of 1:1:1, add normal temperature water, and then add enzymes, every 100g of raw materials (crushed The mixed raw material of cinnamon, oregano and thyme) is added 1g of mixed enzyme composed of papain, cellulase and pectinase at a ratio of 1:1:1, wherein the enzymes of papain, cellulase and pectinase The activity is 12U / mg, 0.8U / mg and 1.0U / mg respectively, and the enzymolysis is carried out under certain conditions to obtain the enzymolysis solution. According to the author's research, four factors, such as solid-liquid ratio, enzymatic hydrolysis pH, enzymatic hydrolysis temperature and enzymatic hydrolysis time, which have a great influence on the extraction rate of plant essential oils,...

Embodiment 3

[0111] Embodiment 3, the influence of extraction temperature on subcritical water extraction plant essential oil yield

[0112] 1) Use crushed and sieved cinnamon, oregano, and thyme as a source of raw materials at a ratio of 1:1:1, add room temperature water, and then add enzymes. Add 1g of a mixed enzyme consisting of papain, cellulase and pectinase at a ratio of 1:1:1, wherein the enzyme activities of papain, cellulase and pectinase are 12U / mg, 0.8U / mg and 1.0 U / mg, carry out enzymatic hydrolysis under certain conditions to obtain enzymatic hydrolysis solution. Enzymolysis was carried out according to the optimal enzymolysis conditions in Example 2, that is: the solid-liquid ratio of the crushed plant mixed raw material to normal temperature water was 1g:8mL, the pH value of the enzymolysis was 6.0, and the enzymolysis temperature was 40°C. The time is 50 minutes. Obtain enzymatic solution.

[0113] 2) centrifuging the mixed solution after enzymolysis in step 1) under a ...

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Abstract

The invention discloses plant essential oil with an antibacterial effect as well as a preparation method and application thereof. The method comprises the following steps: (1) crushing plant raw materials, adding water and enzyme for carrying out enzymolysis to prepare an enzymolysis solution; (2) centrifuging the enzymolysis solution, collecting filtrate, putting the filtrate in a water storage tank, collecting filtration residues, putting the filtration residues in a material basket, and carrying out subcritical water extraction on the filtrate and the filtration residues to obtain an extraction solution; and (3) cooling, demulsifying and extracting the extraction solution, collecting organic layers, drying and removing organic solvents to obtain the plant essential oil. The method is simple in process and easy to operate; low-cost and pollution-free water is used as an extraction agent, so that the cost is greatly reduced; the consumption of organic extraction agents is reduced; the method is green and environmental-friendly; in addition, the method also has the characteristics of short time, high extraction efficiency, high extraction yield of essential oil, low energy consumption and high quality of prepared essential oil; the prepared plant essential oil has a super-strong antibacterial effect and wide in application prospect; the extraction yield of the essential oil is greater than or equal to 2.0% and is far higher than that of the existing extraction and separation techniques of the plant essential oil at home and abroad.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a plant essential oil with antibacterial effect and its preparation method and application. Background technique [0002] In order to pursue economic returns, the growth-promoting effect of feed additives in breeding production has been overemphasized in the past, while the long-term harm caused by drug residues in animal products and drug resistance of pathogens has been ignored. With the European Union, the United States and other countries and regions banning or restricting the use of hormones and antibiotics, the development of pollution-free animal husbandry, the production of green animal products, and the enhancement of the market competitiveness of animal products have become the primary tasks of animal husbandry producers. The new pollution-free and safe biological feed additives of antibiotic growth promoters have also become an urgent task for the green bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02
CPCC11B9/025
Inventor 刘明杨凤娟冯占雨董彦君孙敏戴刚
Owner 北京中农弘科生物技术有限公司
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