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Heat-resistant fungus new species and application thereof in microbial fertilizer

A microbial fertilizer and microbial agent technology, applied to filamentous fungi and their application in the preparation of microbial fertilizers, microbial strains and their application in the agricultural field, to achieve high enzyme activity, good thermal stability, and simple cultivation conditions

Active Publication Date: 2016-12-14
新疆山川秀丽生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research of prior art shows that there are relatively few studies on the biological control and microbial fertilizer of silvery mildew, and silvery mildew is a kind of potential biological control of plant nematodes. Therefore, it is of practical significance to study and utilize the characteristics of this microorganism

Method used

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  • Heat-resistant fungus new species and application thereof in microbial fertilizer
  • Heat-resistant fungus new species and application thereof in microbial fertilizer
  • Heat-resistant fungus new species and application thereof in microbial fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation, screening and identification of Cunninghamella sp. TF121

[0035] 1. Separation: It was obtained from the natural composting process samples of sheep manure around sheep pens in Turpan, Xinjiang. The sample adopts the gradient dilution method, weighs 10g of the irradiated soil sample, puts it in 90ml of sterile physiological saline, activates it at 28°C-32°C for 30min-2h, then performs gradient dilution, and spreads it on a PDA medium plate for cultivation.

[0036] 2. Purification: after the growth of fungal colonies, pick out colonies of different shapes, sizes, colors, etc., and inoculate them on corresponding plates, and purify and cultivate them at 40°C-60°C, preferably at 55°C until there are no foreign colonies. Strains are preferred.

[0037] 3. Classification and identification: By observing the colony shape and physiological and biochemical characteristics, the total DNA of the strain TF121 was extracted, and the fungal ITS PCR amplifica...

Embodiment 2

[0044] Example 2: Analysis of Cunninghamella sp. TF121 CGMCC No.12624 Functional Enzyme

[0045] Using the transparent circle method, plant Cunninghamella sp. TF121 CGMCC No.12624 on the cellulase screening medium ((NH 4 ) 2 SO4 2.0g, MgSO 4 0.5g, KH 2 PO 4 1.0g, CMC-Na 2.0g, Congo red 0.4g, agar 15.0g, distilled water 1L, pH natural), amylase screening medium (soluble starch 20.0g, KCl 0.5g, NaNO 3 2.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, agar 15.0g, distilled water 1L, pH 6.5), lipase screening medium (KH 2 PO4 0.2g, MgSO 4 0.05g, PVA emulsion 2.0mL, yeast powder 0.2g, peptone 1.0g, agar 1.7g, distilled water 1L, pH 6.5. Sterilize and cool at 50°C, add 10mL0.2% rhodamine B solution), protease screening medium (peptone 10.0g, glucose 1.0g, CaCl 2 0.1g, tyrosine 0.1g, casein 5.0g, agar 15.0g, distilled water 1L, pH 6.5, chitosan enzyme screening medium (chitosan 10.0g, (NH 4 ) 2 SO 4 2.5g, MgSO 4 ·7H 2 O 0.25g, K 2 HPO 4 0.02g, CaCO 3 6.0g, agar 20.0, pH 6.0...

Embodiment 3

[0049] Example 3: Determination of parasitism of root-knot nematode eggs by heat-resistant Cunninghamella sp. TF121 CGMCC No.12624

[0050] The isolated and purified Cunninghamella sp. TF121 CGMCC No.12624 was cultured on the slant of PDA medium for 3 days, and then 5 mL of sterile water was added to the slant test tube, shaken vigorously, and then filtered with double-layer sterile gauze. The filtrate was prepared by appropriate dilution to contain 10 6 A spore suspension was used for later use.

[0051] Select a glass petri dish with good light transmittance, add sterilized 0.1% chloramphenicol water agar (distilled water 1L, agar 10g, add chloramphenicol after sterilization to a final concentration of 0.1%), and make a thickness of about 1.5mm Flat TLC. Add 10 μL of spore suspension of Cunninghamella sp. TF121 CGMCC No.12624 dropwise under sterile conditions on the water agar plate. Root-knot nematode egg suspension from the source, the petri dish was sealed with parafil...

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Abstract

The invention discloses a heat-resistant fungus new species and application thereof in a microbial fertilizer. A heat-resistant fungus new species TF121 is obtained through selecting from a sheep manure natural compost specimen in a Turpan area of Sinkiang; through preparing a microbial fertilizer, a parasitic effect is realized on Meloidogynesp. eggs of tested cotton, apple, tomato, cucumber and eggplant sources, an egg parasitism rate is 22.1 percent to 32.2 percent, and an average parasitism rate is 26.6 percent. According to an effect experiment applied in cotton and greenhouse tomato crops, the screened Cunninghamella new species TF121 is utilized, so that a crop yield effect is remarkably improved, an obvious production and efficiency increasing effect is achieved after the new species is applied in commercial crops such as greenhouse cucumbers and greenhouse eggplants and fruit trees such as apple trees and bergamot pear trees, meanwhile, the morbidity of root-knot nematode can be effectively reduced, and therefore, the development and the application of a preparation prepared by utilizing the cunninghamella echinulate new species TF121 provided by the invention have a larger application prospect.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applied agriculture, in particular to the technical field of a filamentous fungus and its application in the preparation of microbial fertilizers. Background technique [0002] The genus Cunningella is a type of filamentous fungus, which can be divided into 5 species according to the morphological and physiological characteristics: C. chinensis (short thorns) C. cingae, C. bancroft, C. brucei, Brazil nut Silvery mildew and Chili (Yazhi) Silvery mildew are a family of Mucorales. They are mainly saprophytic. In addition to living in the soil, they also grow in feces, decaying plants and other substrates. [0003] Root-knot nematode (Meloidogynesp.) occurs very commonly in my country, seriously endangers crops, and is difficult to control. With the improvement of people's ecological awareness and the restriction of highly toxic chemical pesticides, the isolation and screening of high-e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C05G3/02C12R1/645C12R1/125C12R1/885C05G3/60
CPCC05F3/00C12N1/14C12N1/20C05G3/60C12N1/145C12R2001/645C05F11/00C05F11/02C05F11/08C05F17/00
Inventor 王晓桦张謇汪玲许咏梅杨金钰栾作涛冯新玉崔艺凡李新梅唐鹏王春燕唐天成
Owner 新疆山川秀丽生物有限公司
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