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Purple perilla seed anti-oxidizing heptapeptide and preparation method of same

A perilla seed and antioxidant technology, applied in food ingredients as antioxidants, peptides, food preservation, etc., can solve problems such as less research on protein development and utilization, and no reports on active peptide pharmacology.

Active Publication Date: 2016-12-21
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, domestic and foreign researches on perilla seeds mainly focus on oil extraction and biological activity, while there are few studies on the development and utilization of proteins rich in its by-products, and there are almost no reports on its active peptides or their pharmacological studies at home and abroad.

Method used

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  • Purple perilla seed anti-oxidizing heptapeptide and preparation method of same
  • Purple perilla seed anti-oxidizing heptapeptide and preparation method of same
  • Purple perilla seed anti-oxidizing heptapeptide and preparation method of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The separation and purification of the antioxidant heptapeptide of the present invention includes two steps of Sephadex G-25 gel filtration chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC).

[0026] Preparation of perilla seed crude protein enzymatic hydrolysis product: first degrease perilla seed with petroleum ether, then use alkali extraction and acid precipitation method to obtain perilla seed crude protein, use alkaline protease to enzymatically hydrolyze perilla seed crude protein, use alkali Protease was hydrolyzed for 5.00h under the optimal hydrolysis conditions (pH 9.80, enzyme dosage 3000U / g, substrate concentration 3% (w / v)), inactivated in boiling water bath for 10min, centrifuged at 12000rpm for 10min, and taken The supernatant is freeze-dried, which is the enzymatic hydrolyzate of perilla seed protein;

[0027]Sephadex G-25 Gel Filtration Chromatography: Dissolve the freeze-dried powder of perilla seed enzymatic hydrolyza...

Embodiment 2

[0032] The natural antioxidant heptapeptide activity obtained in Example 1 is studied:

[0033] ABTS free radical scavenging effect: prepare 7 mmol / L ABTS with distilled water + solution and 2.45 mmol / L potassium persulfate solution (must be placed at room temperature for 16 h before use), ABTS + Mix with potassium persulfate solution at a volume ratio of 1:1, and dilute the mixture to the absorbance value A with pH 7.4, 5 mmol / L phosphate buffer solution before use 734 is 0.70±0.02. Take 0.5 mL of samples with different concentrations and 0.5 mL of ABTS radical solution, mix and let stand for 10 min, then measure the absorbance at 734 nm. Deionized water and reduced glutathione were used instead of samples as blank control and positive control, respectively. ABTS free radical scavenging activity is calculated according to the following formula (1):

[0034] (1)

[0035] In the formula, A 0 : Absorbance value of blank control group; A s : Absorbance value of the sam...

Embodiment 3

[0047] The natural antioxidant heptapeptide that obtains in embodiment 1 is carried out cytotoxicity and antioxidation research:

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Abstract

The invention provide a purple perilla seed anti-oxidizing heptapeptide and a preparation method of same. The anti-oxidizing heptapeptide has the following sequence: Ser-Gly-Pro-Val-Gly-Leu-Trp (SGPVGLW). An in-vitro test proves that the heptapeptide can effectively remove ABTS and has a strong oxygen radical absorbance capacity (ORAC); and meanwhile, the heptapeptide can effectively inhibit lipid peroxidization of linoleic acid. A cellular test proves that the heptapeptide is safe to cells, and has significant inhibiting effects on oxidative damage of HepG-2 cells. The anti-oxidizing heptapeptide has simple structure, is safe and has strong anti-oxidizing activity, can be used as an excellent replacement of artificially synthesized anti-oxidizing agent, and has an important value in the fields of development and application of novel anti-oxidizing health-caring products and food additives.

Description

technical field [0001] The invention relates to a perilla seed natural antioxidant heptapeptide and a preparation method thereof, belonging to the field of food biotechnology. Background technique [0002] my country is a large agricultural country with a wide variety of crops. However, due to the limitation of processing technology, most of the processing of crops is in the primary stage, and the residues after processing still have a large amount of nutrients, such as protein, etc., and they are Most of them are mainly used in animal feed or fertilizer industry and natural discharge, which not only wastes resources, but also causes environmental pollution. The introduction of the concept of plant active peptides and the improvement of separation and detection technology have made people gradually pay attention to the deep processing of protein-rich agricultural by-products and obtain a variety of active peptides, such as casein phosphopeptides, blood pressure-lowering pepti...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06A23L5/20A23L3/3544
CPCA23L3/3544A23V2002/00C07K7/06C12P21/06A23V2200/02A23V2250/21A23V2250/55
Inventor 洪晶胡磊王惠敏孟春
Owner FUZHOU UNIV
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