Preparation method and application of a kind of Pichia membranosa strain and its biological agent

A technology of Pichia pastoris and microbial agent, which is applied in the field of agriculture, can solve problems such as the adverse effects of the surrounding environment, the reduction of moisture content, and the loss of nutrients, so as to improve the treatment and utilization effect, promote the nitrogen retention and deodorization of materials, and reduce the The effect of volatilization or loss

Active Publication Date: 2019-10-22
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the production of commercial organic fertilizer by aerobic fermentation and composting is still the most important disposal and utilization method of agricultural waste (mainly referring to livestock and poultry manure) in my country. After agricultural waste is composted by harmless high-temperature fermentation, the moisture content is greatly reduced, harmful pathogenic microorganisms are basically killed, and transportation and application are more convenient. However, the quality of organic fertilizer products must meet the "NY 525-2012 Organic Fertilizer" standard. However, during the composting process, the raw materials such as livestock and poultry manure will volatilize and produce a large amount of ammonia and other odorous gases, which makes the waste disposal site and the actual working environment of the workers very poor, causing adverse effects on the surrounding environment, and the nitrogen loss of the processed materials. Larger, affecting the further improvement of the quality of organic fertilizer products; At present, the ammonia volatilization and odor problems generated in the disposal of agricultural waste (mainly livestock and poultry manure) in China are still dominated by measures such as acid adjustment, ventilation, and suction filtration and adsorption. However, the environmental problems of nitrogen loss and odor pollution still exist. Therefore, the development of nitrogen preservation and deodorization technology for agricultural waste disposal by microbial agents has attracted widespread attention in the industry.
[0003] Substances with high nitrogen content are often accompanied by nitrogen loss during the composting process, which will not only cause the loss of nutrients, but also cause pollution to the surrounding environment
At present, the development and development of microbial agents for the control of nitrogen loss in composting is still rare

Method used

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  • Preparation method and application of a kind of Pichia membranosa strain and its biological agent
  • Preparation method and application of a kind of Pichia membranosa strain and its biological agent
  • Preparation method and application of a kind of Pichia membranosa strain and its biological agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Nitrogen-conserving and deodorizing test: Spray nitrogen-containing bacteria agent (microbial agent prepared by Pichia membranosa strain) test with 4 treatments, namely control CK (tap water), treatment T1 (diluted 100-fold bacterial agent), and treatment T2 ( 10-fold dilution of bacterial agent) and processing T3 (bacterial agent stock solution), the test was carried out in a cultivation and treatment greenhouse. Each treatment tank is 2.25m wide, 4 meters long, and the interval between treatments is 2m; directly pour 400kg of pig manure into the tank and spread it out, and the pig manure material is adjusted to a moisture content of about 65% with squeezed manure and wheat bran, and the upper layer is spread Sprinkle extruded feces with a thickness of 2cm, the total thickness of the material is 6-8cm, the application rate of nitrogen-enhancing bacteria is 1% (V / W), evenly spray on the surface of the material, and then inoculate the fly maggot larvae at a rate of 7.5‰ (...

Embodiment 2

[0035] Determination method of ammonia volatilization in aquaculture materials: According to the principle of ammonium nitrogen determination, the volatilization of ammonia can be absorbed by a fixed volume of boric acid absorption solution (containing specific methyl red and bromocresol green indicator, which is wine red), After the ammonia is absorbed by the boric acid solution, the boric acid solution gradually turns from red to green. The more the amount of ammonia absorbed, the darker the green of the absorption solution. Then use standard acid to titrate the boric acid absorption solution to wine red. According to the amount of standard acid consumed in the titration The total amount of ammonia absorbed in boric acid can be calculated. During the test, 3 specific ammonia volatilization collection containers (wide-mouthed, containing 250ml boric acid solution) were suspended on the surface of the cultured fly maggot treatment material in each fly maggot culture treatment p...

Embodiment 3

[0038] Example 3 Nitrogen loss

[0039] Table 2 shows the changes in physical and chemical properties of pig manure during the composting process and the nitrogen loss of the nitrogen-containing bacteria agent (microbial agent prepared by Pichia membranosa strains) nitrogen-keeping and deodorizing tests.

[0040] Table 2

[0041]

[0042]

[0043] It can be seen from Table 1 that after 6 days of composting, the nitrogen loss of T3 and T2 treatments was 29.61% and 4.79% lower than that of T0 treatment, respectively, and the nitrogen loss of T2T1 treatment was more, which may be related to the higher initial nitrogen content. After 28 days of composting, the nitrogen losses of T3 and T2 treatments were 21.7% and 4.79% lower than those of T0 treatment, respectively, and after 49 days, the nitrogen losses of T3 and T2 treatments were 14.18% and 4.26% lower than those of T0 treatment, respectively. It shows that the nitrogen-conserving and deodorizing bacteria agent has a ce...

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Abstract

The invention discloses a preparation method and application of a Pichia membranosa strain and its biological agent. The strain has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on January 27, 2016, and the preservation number is CGMCC. No.12112; taxonomy named Pichia membranifaciens. The invention also discloses the microbial agent produced by the bacterial strain and its preparation method. The Pichia membranosa and its microbial agent provided by the present invention have strong reproductive adaptability, can reduce the volatilization or loss of nitrogen in agricultural waste disposal, increase the total nitrogen in agricultural waste compost materials, and can promote material conservation. Nitrogen deodorization; improving the treatment and utilization of agricultural waste.

Description

technical field [0001] The invention belongs to the technical field of agriculture and discloses a preparation method and application of a Pichia membranosa strain and its biological agent. Background technique [0002] At present, the production of commercial organic fertilizer by aerobic fermentation and composting is still the most important disposal and utilization method of agricultural waste (mainly referring to livestock and poultry manure) in my country. After agricultural waste is composted by harmless high-temperature fermentation, the moisture content is greatly reduced, harmful pathogenic microorganisms are basically killed, and transportation and application are more convenient. However, the quality of organic fertilizer products must meet the "NY 525-2012 Organic Fertilizer" standard. However, during the composting process, the raw materials such as livestock and poultry manure will volatilize and produce a large amount of ammonia and other odorous gases, which m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C05F15/00C05F17/00C12R1/84
CPCC05F3/00C12N1/16C05F17/05C12N1/165C12R2001/84C05F5/002C05F11/08Y02W30/40
Inventor 王卫平朱凤香姚燕来洪春来陈晓旸薛智勇
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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