Preparation method and application of a kind of Pichia membranosa strain and its biological agent

Abstract

The invention discloses a preparation method and application of a Pichia membranosa strain and its biological agent. The strain has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on January 27, 2016, and the preservation number is CGMCC. No.12112; taxonomy named Pichia membranifaciens. The invention also discloses the microbial agent produced by the bacterial strain and its preparation method. The Pichia membranosa and its microbial agent provided by the present invention have strong reproductive adaptability, can reduce the volatilization or loss of nitrogen in agricultural waste disposal, increase the total nitrogen in agricultural waste compost materials, and can promote material conservation. Nitrogen deodorization; improving the treatment and utilization of agricultural waste.

Description

technical field [0001] The invention belongs to the technical field of agriculture and discloses a preparation method and application of a Pichia membranosa strain and its biological agent. Background technique [0002] At present, the production of commercial organic fertilizer by aerobic fermentation and composting is still the most important disposal and utilization method of agricultural waste (mainly referring to livestock and poultry manure) in my country. After agricultural waste is composted by harmless high-temperature fermentation, the moisture content is greatly reduced, harmful pathogenic microorganisms are basically killed, and transportation and application are more convenient. However, the quality of organic fertilizer products must meet the "NY 525-2012 Organic Fertilizer" standard. However, during the composting process, the raw materials such as livestock and poultry manure will volatilize and produce a large amount of ammonia and other odorous gases, which m...

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Problems solved by technology

[0002] At present, the production of commercial organic fertilizer by aerobic fermentation and composting is still the most important disposal and utilization method of agricultural waste (mainly referring to livestock and poultry manure) in my country. After agricultural waste is composted by harmless high-temperature fermentation, the moisture content is greatly reduced, harmful pathogenic microorganisms are basically killed, and transportation and application are more convenient. However, the quality of organic fertilizer products must meet the "NY 525-2012 Organic Fertilizer" standard. However, during the composting process, the raw materials such as livestock and poultry manure will volatilize and produce a large amount of ammonia and other odorous gases, which makes the waste disposal site and the actual working environment of the workers very poor, causing adverse effects on the surrounding environment, and the nitrogen loss of the processed materials. Larger, affecting the further improvement of the quality of organic fertilizer products; At present, the ammonia volatilization and odor problems generated in the disposal of agricultural waste (mainly livestock and poultry manure) in China are still dominated by measures such as acid adjustment, ventilation, and suction filtration and adsorption. However, the environmental problems of nitrogen loss and odor pollution still exist. Therefore, the development of nitrogen preservation and deodorization technology for agricultural waste disposal by microbial agents has attracted widespread attention in the industry.

[0003] Substances with high nitrogen content are often accompanied by nitrogen loss during the composting process, which will not only cause the loss of nutrients, but also cause pollution to the surrounding environment

At present, the development and development of microbial agents for the control of nitrogen loss in composting is still rare

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