Detection method for high throughput liquid chromatogram tandem mass spectrum and method for detecting four catecholamine metabolites

Abstract

The invention discloses a detection method for high throughput liquid chromatogram tandem mass spectrum. The method comprises the following steps: (S1) preparing a sample; (S2) performing derivatization reaction; (S3) pretreating the sample; (S4) separating the liquid chromatogram; and (S5) detecting the tandem mass spectrum. Besides, the invention also discloses a method for detecting four catecholamine metabolites through a high throughput liquid chromatogram tandem mass spectrometry. The detection method provided by the invention is fit for the detection for the catecholamine metabolites in human plasma and has the advantages of simple sample pretreatment, capability of simultaneously detecting four catecholamine metabolites, excellent detection specificity, short time in the whole detection process and high throughput.

Description

technical field [0001] The invention relates to the technical field of catecholamine metabolite detection, in particular to a high-throughput liquid chromatography tandem mass spectrometry detection method and a high-throughput liquid chromatography tandem mass spectrometry method for detecting four catecholamine metabolites. Background technique [0002] Catecholamine is a neurological substance containing catechol and amine groups, including epinephrine (epinephrine, E), norepinephrine (norepinephrine, NE), and dopamine (dopamine, DA), which are produced by the adrenal medulla and some sympathetic neurons. It is a very important neurotransmitter secreted by neuronal chromaffin cells, and it is also an important hormone substance. These three catecholamines are all converted from tyrosine. After the metabolism of catecholamines in the body, they are converted into corresponding metabolites such as methoxyepinephrine (or metanephrine, Metanephrine, MN), methoxyl Norepinephr...

AI Technical Summary

Problems solved by technology

[0004] At present, the commonly used detection methods of catecholamine metabolites mainly include radioenzyme method, chemiluminescence method, fluorescence method and high performance liquid chromatography, etc. These detection methods mainly have the following problems: first, the pretreatment process of plasma samples is complicated, and A large amount of initial sample is required; second, the existing detection technology takes a long time, has large experimental errors, and low throughput; third, since epinephrine and methoxynorepinephrine have the same molecular weight and very similar structure, it is impossible to Conventional detection methods are used to distinguish, and the specificity is poor; fourth, the existing detection methods usually can only detect one or two of them, and cannot achieve high-throughput simultaneous detection of four catecholamine metabolites

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