Detection method for high throughput liquid chromatogram tandem mass spectrum and method for detecting four catecholamine metabolites

A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of detection of catecholamine metabolites, can solve the problems of complex processing, large sample volume, large experimental error, etc., and achieve the effects of high detection sensitivity, short detection time and low cost

Active Publication Date: 2016-12-21
HANGZHOU BAICHEN MEDICAL LAB CO LTD
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Problems solved by technology

[0004] At present, the commonly used detection methods of catecholamine metabolites mainly include radioenzyme method, chemiluminescence method, fluorescence method and high performance liquid chromatography, etc. These detection methods mainly have the following problems: first, the pretreatment process of plasma samples is complicated, and A large amount of initial sample is required; second, the existing detection technology takes a long

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  • Detection method for high throughput liquid chromatogram tandem mass spectrum and method for detecting four catecholamine metabolites
  • Detection method for high throughput liquid chromatogram tandem mass spectrum and method for detecting four catecholamine metabolites
  • Detection method for high throughput liquid chromatogram tandem mass spectrum and method for detecting four catecholamine metabolites

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[0055] In order to enable those skilled in the art to better understand the present invention, the protection scope of the present invention is clearly defined; the present invention will be further described in detail below in conjunction with specific embodiments.

[0056] Four kinds of catecholamines (methoxyepinephrine, methoxynorepinephrine, vanillyl mandelic acid, homovanillic acid) standards and corresponding isotope-labeled internal standards (deuterated vanillylmandelic acid, D3-VMA, deuterated formazan Oxyepinephrine, D3-MN) were purchased from Sigma Aldrich Company. Standards and internal standards are first dissolved in acetonitrile, and then stored at -80°C. When actual sample detection is required, these internal standards are diluted to a concentration of about 25nM with acetonitrile as a working solution.

[0057] 1. Standard product preparation: Take 0.3ml of 8 different concentrations of catecholamine metabolite acetonitrile solution into 1.5ml EP tubes, and ...

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Abstract

The invention discloses a detection method for high throughput liquid chromatogram tandem mass spectrum. The method comprises the following steps: (S1) preparing a sample; (S2) performing derivatization reaction; (S3) pretreating the sample; (S4) separating the liquid chromatogram; and (S5) detecting the tandem mass spectrum. Besides, the invention also discloses a method for detecting four catecholamine metabolites through a high throughput liquid chromatogram tandem mass spectrometry. The detection method provided by the invention is fit for the detection for the catecholamine metabolites in human plasma and has the advantages of simple sample pretreatment, capability of simultaneously detecting four catecholamine metabolites, excellent detection specificity, short time in the whole detection process and high throughput.

Description

technical field [0001] The invention relates to the technical field of catecholamine metabolite detection, in particular to a high-throughput liquid chromatography tandem mass spectrometry detection method and a high-throughput liquid chromatography tandem mass spectrometry method for detecting four catecholamine metabolites. Background technique [0002] Catecholamine is a neurological substance containing catechol and amine groups, including epinephrine (epinephrine, E), norepinephrine (norepinephrine, NE), and dopamine (dopamine, DA), which are produced by the adrenal medulla and some sympathetic neurons. It is a very important neurotransmitter secreted by neuronal chromaffin cells, and it is also an important hormone substance. These three catecholamines are all converted from tyrosine. After the metabolism of catecholamines in the body, they are converted into corresponding metabolites such as methoxyepinephrine (or metanephrine, Metanephrine, MN), methoxyl Norepinephr...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062G01N2030/065G01N2030/067
Inventor 吴超超彭军高强刘鹏胡志飞江思
Owner HANGZHOU BAICHEN MEDICAL LAB CO LTD
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