Use of Lactobacillus johnsonii in food calling

A technique for introducing Lactobacillus johnsonii into Lactobacillus johnsonii, applied in the application field of Lactobacillus johnsonii in attracting food

Active Publication Date: 2017-01-04
BEIJING DABEINONG TECH GRP CO LTD +2
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are precedents for the use of lactic acid bacteria in the aquaculture industry, some as feed additives, some as water quality improvers, and some as micro-ecological improvers, but there is no precedent for the use of lactic acid bacteria to attract food.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of Lactobacillus johnsonii in food calling
  • Use of Lactobacillus johnsonii in food calling
  • Use of Lactobacillus johnsonii in food calling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 Lactobacillus johnsonii CGMCC No.4926 fermented liquid

[0039] 1) Activate cryopreserved Lactobacillus johnsonii CGMCC No.4926 on the MRS agar plate for three times, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, culture it at 37°C for 10 hours, then transfer it to 250mL MRS with 1% inoculum Liquid medium, used as seed liquid after culturing for 10 hours;

[0040] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum size of 5%;

[0041] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment for 14 hours under the condition of 100rpm, N 2 , to maintain dissolved oxygen less than 1%;

[0042] 4) When the pH of the feed liquid no longer decreases or the absor...

Embodiment 2

[0043] Application of embodiment 2 Lactobacillus johnsoni in the attraction of Penaeus vannamei

[0044] 1. Test material

[0045]Basic feed: fish meal 16%, soybean meal 20.3%, puffed soybean 4%, peanut meal 21.05%, corn gluten meal 3%, hemoglobin meal 3%, flour 19.87%, shrimp shell powder 2%, brewer's yeast 5%, Squid paste 1.5%, fish oil 0.8%, phospholipid oil 2.4%, choline chloride 0.3%, calcium dihydrogen phosphate 1%, antifungal agent 0.05%, antioxidant 0.03%, shelling factor 0.01%, premix 5% The composition is made into pellets by feed processing equipment.

[0046] Attractant substances: Lactobacillus johnsonii preparations, DMPT (dimethyl-β-thiatin acetate), glycine

[0047] 2. Experimental design

[0048] Two glass aquariums (1.0m×0.5m×0.5m) were placed side by side, separated by dark partitions so that they would not interfere with each other. Put about 240L water in each tank, keep the water temperature at 25°C, NH 3 -N5mg / L, 12 hours of light, the luminosity of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a use of Lactobacillus johnsonii CGMCC No. 4926 in food calling on prawns. The Lactobacillus johnsonii preparation can improve prawn feed intake by about one time and substantially improve a prawn feed intake rate. Compared with the existing feeding promoting agent, the Lactobacillus johnsonii preparation has better food calling effects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an application of Lactobacillus johnsonii in food attraction. Background technique [0002] Lactobacillus johnsonii belongs to the phylum Firmicutes, the class Bacilli, the order Lactobacillales, the family Lactobacillaceae, and the genus Lactobacillus. The European Food Safety Authority (EFSA) issued three health declarations against Lactobacillus johnsonii in 2009 and 2011 respectively, stating that Lactobacillus johnsonii has microorganisms that reduce intestinal pathogens, enhance natural defense and immune system and maintain skin healthy. Moreover, the European Union has conducted a safety qualification accreditation (qualified presumption of safety, QPS) for Lactobacillus johnsonii to approve its use in feed and food. In 2010, the General Office of the Ministry of Health of my country included Lactobacillus johnsonii in the "List of strains that can be used in fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/10A23K1/14A23K1/175A23K1/16C12N1/20C12R1/225
CPCY02A40/818
Inventor 汪攀张军孙晓雯闫轶洁王安如朱传忠李栋
Owner BEIJING DABEINONG TECH GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap