Use of Lactobacillus johnsonii in food calling
A technique for introducing Lactobacillus johnsonii into Lactobacillus johnsonii, applied in the application field of Lactobacillus johnsonii in attracting food
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Embodiment 1
[0038] The preparation of embodiment 1 Lactobacillus johnsonii CGMCC No.4926 fermented liquid
[0039] 1) Activate cryopreserved Lactobacillus johnsonii CGMCC No.4926 on the MRS agar plate for three times, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, culture it at 37°C for 10 hours, then transfer it to 250mL MRS with 1% inoculum Liquid medium, used as seed liquid after culturing for 10 hours;
[0040] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum size of 5%;
[0041] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment for 14 hours under the condition of 100rpm, N 2 , to maintain dissolved oxygen less than 1%;
[0042] 4) When the pH of the feed liquid no longer decreases or the absor...
Embodiment 2
[0043] Application of embodiment 2 Lactobacillus johnsoni in the attraction of Penaeus vannamei
[0044] 1. Test material
[0045]Basic feed: fish meal 16%, soybean meal 20.3%, puffed soybean 4%, peanut meal 21.05%, corn gluten meal 3%, hemoglobin meal 3%, flour 19.87%, shrimp shell powder 2%, brewer's yeast 5%, Squid paste 1.5%, fish oil 0.8%, phospholipid oil 2.4%, choline chloride 0.3%, calcium dihydrogen phosphate 1%, antifungal agent 0.05%, antioxidant 0.03%, shelling factor 0.01%, premix 5% The composition is made into pellets by feed processing equipment.
[0046] Attractant substances: Lactobacillus johnsonii preparations, DMPT (dimethyl-β-thiatin acetate), glycine
[0047] 2. Experimental design
[0048] Two glass aquariums (1.0m×0.5m×0.5m) were placed side by side, separated by dark partitions so that they would not interfere with each other. Put about 240L water in each tank, keep the water temperature at 25°C, NH 3 -N5mg / L, 12 hours of light, the luminosity of...
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