Preparation method for phellinus linteus healthcare wine
A production method and technology of health-care wine are applied in the production field of Phellinus linteus health-care wine, which can solve the problems of long period of artificial cultivation of fruiting bodies, short production period of liquid fermentation, high requirements for equipment and technology, and achieve better taste and golden color. , nutritionally rich effect
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Embodiment 1
[0026] A kind of preparation method of Phellinus Phellinus health wine that present embodiment relates to comprises the following steps:
[0027] (1) Preparation of Phellinus liquid strain:
[0028] Aseptically inoculate the mother species of Phellinus Phellinus into a liquid medium for shaking culture, the culture conditions are: temperature 27°C, shaker speed 150r / min, and vibration culture for 5 days in the dark, to obtain the Phellinus liquid strain; The composition of the liquid medium is: corn flour 40g / L, glucose 25g / L, yeast extract 10g / L, bran 20g / L, KH 2 PO 4 1.25g / L, MgSO 4 ·7H 2 O 0.625g / L, the balance of deionized water;
[0029] (2) Preparation of solid fermentation substrate:
[0030] Select millet with shell (millet, scientific name: Setaria italica ), hulled sorghum, and hulled buckwheat as hulled grains; formula: 98% of hulled grains, 1% of food additive calcium sulfate, and 1% of sucrose; the stated % is a percentage by mass;
[0031] Steps include:...
Embodiment 2
[0043] (1) Fermentation culture: select a 240mL tissue culture bottle, fill the solid fermentation substrate 2 to 1 / 4-1 / 5 of the total volume of the container, and seal it with a lid with a vent filter; then transfer the tissue culture bottle to a high-pressure In a steam sterilizer, sterilize at 121°C for 1.5-2 hours; when the temperature of the solid fermentation substrate 2 drops below 30°C, insert the Phellinus liquid strain prepared in Example 1 with an inoculation amount of 2-3mL / bottle, Cultivate for 20 days at a constant temperature of 25-30°C and away from light;
[0044] (2) Preparation of Phellinus Health Wine:
[0045]1. Add 200mL of 55° white wine to the tissue culture bottle after the fermentation culture is completed, seal the bottle cap with no venting filter membrane, mix well, let stand and extract for 15 days to obtain a solid-liquid mixture;
[0046] 2. The solid-liquid mixture is subjected to solid-liquid separation, and the supernatant is collected to pr...
Embodiment 3
[0051] (1) Fermentation culture: select a 240mL tissue culture bottle, fill the solid fermentation substrate 3 to 1 / 4-1 / 5 of the total volume of the container, and seal it with a lid with a vent filter; then transfer the tissue culture bottle to a high pressure In a steam sterilizer, sterilize at 121°C for 1.5-2 hours; when the temperature of the solid fermentation substrate 3 drops below 30°C, insert the Phellinus liquid strain prepared in Example 1 with an inoculation amount of 2-3mL / bottle, Cultivate for 20 days at a constant temperature of 25-30°C and away from light;
[0052] (2) Preparation of Phellinus Health Wine:
[0053] 1. Add 200mL of 55° white wine to the tissue culture bottle after the fermentation culture is completed, seal the bottle with a bottle cap without a ventilating filter membrane, mix well, let stand for leaching for 15 days, and obtain a solid-liquid mixture;
[0054] 2. The solid-liquid mixture is subjected to solid-liquid separation, and the supern...
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