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Use of mesenchymal stem cell conditioned culture medium for cosmetics field

A conditioned medium and mesenchymal stem cell technology, applied in the field of mesenchymal stem cell conditioned medium, can solve the problem of applying cosmetic products without mesenchymal stem cell conditioned medium

Active Publication Date: 2017-01-11
上海朗斯生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, as far as the inventors know, there is no technology for applying the conditioned medium of mesenchymal stem cells to cosmetics.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of Mesenchymal Stem Cell Conditioned Medium

[0035] Subcutaneous adipose tissue was obtained from young healthy women by liposuction and washed 3-5 times with phosphate buffered saline (PBS, pH 7.4) to remove blood cells and tissue debris. Treat with 0.075% collagenase (type I) at 37° C. for 30-45 minutes, and then centrifuge at 250-500 g for about 10 minutes. The cell pellet obtained by centrifugation was suspended in PBS and filtered through a filter membrane with a pore size of 100 μm. The resulting precipitate was washed again with PBS for 2-3 times to obtain adipose-derived mesenchymal stem cells.

[0036] The obtained adipose-derived mesenchymal stem cells were suspended in the complete medium of DMEM or α-MEM (low sugar) containing 10-20% fetal bovine serum (FBS), 100IU / ml penicillin and 100IU / ml streptomycin, and then 2-20x10 5 / cm 2 The cell density was inoculated into culture flasks and placed at 37°C with 5% CO 2 Cultured in a cel...

Embodiment 2

[0041] Embodiment 2: wrinkle removal experiment

[0042] Experimental group: Take a bottle of the conditional medium CM1 and CM2 prepared in Example 1, after melting at room temperature, add 2mL 0.05mol / L containing 0.9% NaCl and 0.0015mol / LCaCl 2 Phosphate buffer (pH 7.2) diluted.

[0043] Control group: use 2mL 0.05mol / L and contain 0.9% NaCl and 0.0015mol / LCaCl 2 Phosphate buffer (pH 7.2) served as placebo.

[0044] Experimental method: 45 women aged 35-45 were randomly divided into 3 groups, 15 people in each group. The conditioned medium (CM1 and CM2) of the above-mentioned experimental group and the placebo of the control group were used to smear around the orbit of the test subject respectively, and then massage the smeared part for 10 minutes. Twice a day for 3 months. Finally, the wrinkle-removing effects of the experimental group and the control group were evaluated, and the results are shown in Table 1 below.

[0045] Table 1: Results of wrinkle removal experim...

Embodiment 3

[0048] Embodiment 3: whitening experiment

[0049] A circle with a diameter of 3 cm was marked on the outside of each arm of 15 darker-skinned women aged 20-45. One of the circles was smeared with the CM1 dilution of the conditioned medium in Example 2 (experimental group), and the other circle was smeared with the placebo in Example 2 (control group). Then use ultrasound. 1 time a day, continuous use for 2 months. Finally, the whitening effects of the experimental group and the control group were evaluated, and the results are shown in Table 2 below.

[0050] Table 2: Results of whitening experiment

[0051] test group control group Remarkable effect 13 people 0 people General effect 2 people 0 people no significant effect 0 people 15 people

[0052] Among them, the significant effect means that the whitening effect reaches more than 75%, the effect generally means that the whitening effect is between 40% and 60%, and the insig...

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PUM

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Abstract

The invention relates to a use of a mesenchymal stem cell conditioned culture medium for cosmetics, preferably a use for skin repair and regeneration, whitening, wrinkle resisting and oxidation resisting, and is characterized in that the mesenchymal stem cell conditioned culture medium is prepared by the following method: (1) obtaining mesenchymal stem cells; (2) culturing the mesenchymal stem cells obtained in the step (1) in a complete culture medium; (3) culturing the mesenchymal stem cells obtained in the step (2) in a base culture medium; and (4) centrifuging to remove the mesenchymal stem cells, taking a supernatant, and filtering the supernatant, to obtain the filtrate which is the mesenchymal stem cell conditioned culture medium.

Description

technical field [0001] The present invention relates to the use of mesenchymal stem cell conditioned medium. Specifically, the present invention relates to the use of the conditioned medium of mesenchymal stem cells in the field of cosmetics. [0002] technical background [0003] As we all know, human skin aging is caused by both intrinsic and extrinsic factors. Among them, intrinsic factors refer to the functional degeneration phenomenon that occurs naturally in the human life cycle, for example, the telomeres of cells gradually shorten with age, while the oxidative damage caused by aerobic cell metabolism gradually increases. The specific manifestations are the thinning of the epidermis and dermis of the skin, the flattening of the ridges, the reduction of the vascular network, the thinning of the blood vessel wall, the decrease in the number and dysfunction of fibroblasts in the dermis, and the gradual slowing of the synthesis of collagen fibers, while the collagen is re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K8/98A61Q19/08A61Q19/00A61Q19/02A61P17/02
Inventor 姜宁
Owner 上海朗斯生物工程有限公司
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