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Irpex lacteus and application thereof

A technology of Raketodon albicans and mycelium, which is applied in the directions of application, fungi, chemicals for biological control, etc., to achieve the effect of promoting flowering, promoting the growth of crop root systems, and improving stress resistance.

Active Publication Date: 2017-02-15
山东蓬勃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on R. albicans mainly focuses on its hypoxia resistance (CN104138395A), anti-fatigue effect (CN104138396A) and lignin degradation effect (CN105754881A); , Improving disease resistance and fertilizer utilization has not been reported

Method used

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  • Irpex lacteus and application thereof
  • Irpex lacteus and application thereof
  • Irpex lacteus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and identification of bacterial strains

[0027] 1. Isolation and screening of strains:

[0028] (1) Isolation of strains

[0029] Take fresh Daxinganling wild blueberry root samples and rinse them with running water, dry them with filter paper, cut them into appropriate sizes, wash them with sterile water for 3 times, and put them in 10% H 2 o 2 Sterilize in medium for 10 minutes, rinse 3 times with sterile water, soak in 6% sodium hypochlorite solution for 2 minutes, then rinse 3 to 5 times with sterile water, put it on a sterile filter paper, and blot the surface moisture. Use sterile scissors to cut into root segments of about 1 cm, and inoculate them on the PDA medium after high-temperature and high-pressure sterilization at 121° C. for 30 minutes. After being placed in an incubator at 23°C for 3-10 days, hyphae can be seen growing from the cut edge of the sample, which is repeatedly separated and purified on the plate until each plate conta...

Embodiment 2

[0044] Embodiment 2: the preparation of plant inducer

[0045] The specific preparation method is as follows:

[0046] 1. Fermentation culture:

[0047]The bacterial classification of Irpex lacteus PR2 isolated and preserved in Example 1 preserved on the slant of the test tube at 4°C was placed on the PDA medium, cultivated at 25°C for one week, and the agar was dug into pieces and inoculated in 50mL PDA medium. The 250mL Erlenmeyer flask of culture medium was cultivated at 25°C for 3 days on a rotary shaker at 180r / min as seeds, and inoculated into a 500mL Erlenmeyer flask containing 150mL fermentation medium with 10% of the amount, cultivated for 5 days under the same conditions, and terminated Ferment to obtain seed liquid.

[0048] PDA medium formula: potato 200g, glucose 20g, agar 20g, water 1000ml.

[0049] Fermentation medium formula: 1.0L of potato extract, 1.0g of yeast extract, 3.0g of peptone, 15.0g of glucose, and 17.0g of agar.

[0050] Preparation of potato e...

Embodiment 3

[0053] Embodiment 3: the application of plant inducer in potato growth promotion and production increase

[0054] 1. Test method:

[0055] The potato sowing date is April 18-19, 2015, the sowing density is 3300-3500 plants / mu, the ridge spacing is 55cm, and the plant spacing is 35cm. During the implementation process, the farming operations during the potato growing season are the same as field planting.

[0056] Treatment 1: conventional treatment, that is, when sowing, no endophyte extract (that is, the plant inducer prepared in Example 2) was added to spray the ditch; during the expansion period, amino acid water-soluble fertilizer was drip-irrigated with water.

[0057] Treatment 2: when sowing, endophyte extract (i.e. the plant inducer prepared in Example 2) 10ml / mu spray ditch; in the expansion stage, amino acid water-soluble fertilizer, drip irrigation with water.

[0058] Process 3: when sowing, endophyte extract (i.e. the plant inducer prepared in Example 2) 10ml / mu...

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Abstract

The invention discloses irpex lacteus which is named as irpex lacteus PR2 and is preserved in CGMCC, short for China General Microbiological Culture Collection Center, wherein the address is Institute of Microbiology of Chinese Academy of Sciences, No. 3, Yard No.1, Beichen West Road, Chaoyang District, Beijing, and the culture collection number is CGMCC NO.13190. According to the invention, the irpex lacteus is separated and purified from the root of wild blueberries in Great Khingan for the first time, a large number of hyphae of the irpex lacteus are obtained through liquid aerobic fermentation, and a natural substance capable of promoting the growth of the root system of crops, improving stress resistance, promoting blossom, increasing yield and improving the quality of the crops is obtained by taking ethanol as an extracting solvent and through ultrasonic extraction, so that the further development on a natural compound with ultrahigh growth-promoting activity is benefited; furthermore, no three wastes are generated in the whole production process, so that low cost and high benefit are realized, and the social requirements of environmental protection and grain safety production are met.

Description

technical field [0001] The invention relates to a strain of albicans rake tooth fungus and its application, and belongs to the field of biotechnology. Background technique [0002] With the increase of population and the decrease of arable land, the contradiction between my country's food security, resource consumption and environmental protection has become increasingly acute. Fertilizer plays an important role in agricultural production, but there are some unreasonable factors in the process of use, which lead to the failure of the production performance of fertilizer to be effectively exerted, resulting in the decline of soil fertility, the decrease of fertilizer utilization rate, the increase of agricultural production cost, and the decrease of benefit. It leads to the decline of the quality of agricultural products, pollutes the ecological environment, accelerates the decline of soil quality, and directly affects the continuous increase of grain production, the improvem...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12P1/02C12N1/145C12R2001/645
Inventor 代丽丽
Owner 山东蓬勃生物科技有限公司