Antigen protein of bovine respiration syncytial viruses

Abstract

The invention discloses an antigen protein of bovine respiration syncytial viruses. At least two sites of an amino acid sequence of the antigen protein of the bovine respiration syncytial viruses are substituted by cysteine as compared with SEQ CAA76980.1, the substituted cysteine is connected by disulfide bonds, and internal disulfide bonds, hole filling, single-strain connection and the like are improved on the basis. The invention further provides a method for preparing the antigen protein. The antigen protein and the method have the advantage that technologies and theories of protein crystallography are applied to the antigen protein, structural change of the antigen protein in virus infection and pathogenicity procedures can be determined, genetic engineering modification can be carried out on critical virus proteins according to results of the structural change, accordingly, the virus proteins can become protein antigens with a function of infection without pathogenicity and capability of causing efficient reaction of antibodies of animal bodies, and bovine respiration syncytial virus infection can be effectively prevented and treated; perfect virus vaccine can be developed on the basis, accordingly, loss due to virus infection can be effectively controlled and prevented, and the antigen protein and the method have important economical significance and social significance.

Description

technical field [0001] The invention belongs to the technical field of biological product preparation, and relates to a bovine respiratory syncytial virus antigen protein and a preparation method thereof. Background technique [0002] Bovine Respiration Syncytial Virus (BRSV) infection can cause bovine respiratory diseases, and is listed as an important bovine disease in European Community countries after bovine mucosal disease (BVD) and bovine infectious rhinotracheitis (IBR). one of the diseases. The disease is prevalent worldwide, and its harmfulness is that the incidence of BRSV infection reaches as high as 60%-80%, and the mortality rate reaches more than 20% in some outbreaks, causing great economic losses to the cattle industry. Although this BRSV has been included in the research and development category since the 1970s, ideal drugs and vaccines have not been developed due to various reasons. Although there have been reports on various inactivated, attenuated, reco...

AI Technical Summary

Problems solved by technology

With the continuous advancement of human knowledge, the limitations of traditional vaccines are increasingly revealed: (1) animal and human viruses need to be cultured in animal cells, which makes the cost of vaccine production very high; (2) pathogenic viruses in vaccines The substance may not be completely killed or sufficiently attenuated during the vaccine production process, which will cause the vaccine to contain highly toxic pathogenic substances, which in turn will cause the disease to spread in a wider range; (3) Attenuated strains may occur mutation, disease-causing

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