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Standard sample of malachite green and metabolites thereof in carp flesh, and preparation method and application thereof

A malachite green and standard sample technology, applied in the field of analytical chemistry, can solve the problems affecting the reliability and validity of the results, unable to represent the target analyte, and unable to reflect the accuracy of the method, etc., to achieve long shelf life, no preservatives and anti-corrosion Oxidizing agent, easy storage effect

Inactive Publication Date: 2017-02-22
辽宁省检验检疫科学技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the existing technical analysis methods cannot represent the real state of the target analyte in the living body, cannot truly reproduce the extraction efficiency of the drug in the animal body after natural metabolism, cannot reflect the accuracy of the method, and affect the reliability and reliability of the results. Effectiveness, and there is a technical problem that antioxidants affect the results of use. One purpose of the present invention is to provide the preparation method and application of malachite green and its metabolite standard samples in carp meat

Method used

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  • Standard sample of malachite green and metabolites thereof in carp flesh, and preparation method and application thereof
  • Standard sample of malachite green and metabolites thereof in carp flesh, and preparation method and application thereof
  • Standard sample of malachite green and metabolites thereof in carp flesh, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of preparation method of malachite green and its metabolite standard sample in carp muscle, its concrete steps are as follows:

[0033] (1) Selection of experimental animals: Commercially purchased live carp, requiring uniform individual size and average weight of 1.5kg, raised at 20°C, inflated, water changed every day, and injured and unhealthy carp were removed after 3 days;

[0034] (2) Drug breeding addition: Weigh 4.0g of malachite green in a 500mL beaker, add about 200mL of water, transfer it to a 250mL volumetric flask after complete dissolution, and then make up the volume to prepare an aqueous solution of malachite green with a concentration of 16mg / mL;

[0035] The exposure experiment was carried out by gavage. The 5mL syringe was connected with a hose with a length of about 6cm, which was directly connected to the stomach of the fish. Each fish was fed with 2 mL of the above-mentioned malachite green aqueous solution, that is, the dosage was about 21 ...

Embodiment 2

[0042] In this embodiment, the preparation process in Example 1 is repeated, and the different experimental conditions are:

[0043] 1) The body weight of the experimental animals is 2kg;

[0044] 2) The breeding temperature is 22°C;

[0045] 3) Weigh 400 mg of malachite green;

[0046] 4) The drug dosage is 1.6mg / kg body weight;

[0047] 5) Continuous administration for 3 days;

[0048] 6) Slaughter 3 hours after the last administration;

[0049] 7) Pre-freeze at -80°C for 0.5h, vacuum freeze at 0.01mbar for 8h, and quickly heat up and dry for 2h;

[0050] 8) Pass through a 50-mesh sieve;

[0051] 9) Store in cold storage at 5°C.

Embodiment 3

[0053] In this embodiment, the preparation process in Example 1 is repeated, and the different experimental conditions are:

[0054] 1) The body weight of the experimental animals is 1kg;

[0055] 2) The breeding temperature is 25°C;

[0056] 3) Weigh 3.75g of malachite green;

[0057] 4) The drug dosage is 30mg / kg body weight;

[0058] 5) Continuous administration for 5 days;

[0059] 6) Slaughter 5 hours after the last administration;

[0060] 7) Pre-freezing at -30°C for 10 hours, vacuum freezing at 1.0 mbar for 15 hours, and rapid heating and drying for 10 hours;

[0061] 8) pass through 80 mesh sieve;

[0062] 9) Store in cold storage at 8°C.

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Abstract

The invention provides a standard sample of malachite green and metabolites thereof in carp flesh and a preparation method thereof, belonging to the technical field of analytical chemistry. The preparation method comprises the following steps: cramming malachite green to breed carp, so that the carp body contains the malachite green and metabolites; breeding the carp in drug-free water until the in-vivo drug concentration reaches the stable metabolic status, catching the carp, killing the carp, taking muscles on both sides of the back, making minced fish, carrying out vacuum freeze drying, screening, and packaging in a bottle, thereby obtaining the carp powder standard sample containing malachite green and metabolites thereof. The method solves the problem of great differences between the extraction, purification and other treatment results and the routine analysis sample since the target matter-matrix combination condition is not completely consistent with that of the real detection sample. The standard sample is a material standard sample, is uniform and stable, is free of any preservative or antioxidant, is easy to preserve, and is used for capacity verification of malachite green residue test items, verification of detection methods, calibration of test instruments, and quality control and examination of test results.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to the preparation of a natural matrix standard sample of veterinary drug residues, in particular to a standard sample of malachite green and its metabolites in carp meat, a preparation method and application thereof. Background technique [0002] Malachite green (MG) is a crystal with metallic luster, easily soluble in water, and its aqueous solution is blue-green. It can be used as a bactericide and insect repellent, and it is a kind of medicinal dye with strong antibacterial effect, belonging to triphenylmethane chemical substances. Many countries have used it as a fungicide in the aquaculture industry to control the growth of fungi in fish and fry, and to control high-incidence saprolegniasis and protozoan diseases. Malachite green is rapidly metabolized into colorless malachite green (leucomalachitegreen, LMG, also called recessive malachite green) in aquatic animals. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/2893
Inventor 王秋艳林维宣牛承辉张宁
Owner 辽宁省检验检疫科学技术研究院