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A kind of polysaccharide hybrid manganese dioxide nanoparticles for magnetic resonance imaging and its preparation method and application

A technology of manganese dioxide and nanoparticles, which is applied in the field of biomedical materials, can solve the problems of lack of tumor targeting of manganese dioxide nanoparticles, difficulty in biodegradation of water-soluble polymers, difficulty in separation and purification and mass production, and achieve easy operation, low cost, and good biocompatibility

Active Publication Date: 2018-04-13
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still the following main problems: first, most of the water-soluble polymers used are difficult to biodegrade, and some cationic polymers also have certain biological toxicity; second, the modification involves multi-step reactions, the process is complicated, and it is difficult to separate, purify and mass produce The third is that the modified manganese dioxide nanoparticles lack tumor targeting, and it is necessary to introduce tumor targeting factors with the help of other chemical reactions during practical application.

Method used

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  • A kind of polysaccharide hybrid manganese dioxide nanoparticles for magnetic resonance imaging and its preparation method and application
  • A kind of polysaccharide hybrid manganese dioxide nanoparticles for magnetic resonance imaging and its preparation method and application
  • A kind of polysaccharide hybrid manganese dioxide nanoparticles for magnetic resonance imaging and its preparation method and application

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Embodiment 1

[0028] Gently dissolve 0.1g of sodium hyaluronate (HA, molecular weight 20-50KDa) in 20mL of water at 25°C, dissolve 1mg of potassium permanganate in 5mL of water, mix the polysaccharide aqueous solution and potassium permanganate solution at room temperature Evenly, the resulting mixture was subjected to a hydrothermal reaction at 40 degrees Celsius for 15 minutes, and the ultraviolet-visible spectrogram of the solution was measured in real time by a purple-visible spectrometer, as shown in figure 1 shown. Potassium permanganate solution has characteristic absorption peaks at 315nm, 525nm and 545nm. As time goes by, the three characteristic peaks of potassium permanganate gradually disappear, and at the same time a new characteristic absorption peak appears at about 360nm, indicating that the polysaccharide hybrid Generation of manganese oxide nanoparticles. As the reaction time strengthens, the intensity of this absorption peak also continues to increase. This is because po...

Embodiment 2

[0030] Gently dissolve 1g of sodium hyaluronate (HA, molecular weight 10-30KDa) in 40mL of water at 37°C, dissolve 0.10g of potassium permanganate in 10mL of water, and dissolve the polysaccharide aqueous solution and permanganate solution at 37°C Mixed evenly, the resulting mixture continued to react at 37 degrees Celsius for 2 hours, dialyzed with pure water and then freeze-dried into powder. The valence state of the product was investigated by X-ray photoelectron spectroscopy. Tetravalent manganese can be clearly seen in the high-resolution spectrum. The characteristic absorption peak of the 2p orbital of Mn(IV) further confirmed the formation of manganese dioxide hybrid nanoparticles.

Embodiment 3

[0032] Dissolve 0.01g of sodium hyaluronate (HA, molecular weight 5-10KDa) at room temperature to prepare a solution with a polysaccharide mass fraction of 0.05%, and at the same time dissolve 0.01g of potassium permanganate in 10 mL of water, and mix the polysaccharide aqueous solution with Mix the permanganate solution evenly at 20 degrees Celsius, and continue to react the resulting mixture at 90 degrees Celsius for 12 minutes. After dialyzing and diluting with pure water, take 10 μL and drop it on a 200-mesh copper grid coated with a carbon film. After 1 minute, , blot the excess liquid with filter paper, and use JEM100CX transmission electron microscope (Japan Electronics Corporation, Japan) to observe after drying, the magnification is 50K, observe its microscopic shape by transmission electron microscope, the size is about 80nm, and dynamic light scattering The measurement results are approximately the same.

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Abstract

The invention discloses a polysaccharide hybrid manganese dioxide nano-particle for magnetic resonance imaging and a preparation method and application thereof. The preparation method includes the steps that polysaccharide with excellent water solubility, biocompatibility and biodegradability is used as a chemical reducing agent and a dispersion stabilizer, the polysaccharide and permanganate are subjected to a chemical reaction in a water solution, and the polysaccharide hybrid manganese dioxide nano-particle is prepared through a single-step method. The adopted natural polysaccharide has excellent water solubility, biocompatibility and biodegradability and further has certain tumor targeting. The prepared polysaccharide hybrid manganese dioxide nano-particle is easily and evenly dispersed in a water system, is safe and low in toxicity, has an obvious magnetic relaxation enhancing effect under the tumor tissue micro-environment condition, is especially suitable for magnetic resonance development imaging of tissue parts of tumors such as glioma, liver cancers and breast cancers, and can be chemically coupled with anti-tumor drugs, and then diagnosis and treatment integration is achieved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a polysaccharide hybrid manganese dioxide nanoparticle for magnetic resonance imaging and its preparation method and application. Background technique [0002] Manganese is a paramagnetic transition metal that can be used as a novel contrast agent in magnetic resonance imaging. Compared with gadolinium-based contrast agents commonly used in clinical practice, manganese-based contrast agents have better biological safety. Manganese can exist in various forms such as manganese salts, coordination compounds, and oxide nanoparticles, among which manganese dioxide nanoparticles are the most promising. The reason is that manganese dioxide nanoparticles, as contrast agents for magnetic resonance imaging, can be converted into divalent manganese ions with a relaxation-enhancing effect in the microenvironment of tumor tissues, thereby realizing specific imaging of tumor si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01G45/02B82Y40/00A61K49/12A61K49/18
CPCA61K49/12A61K49/1824C01G45/02C01P2002/84C01P2002/85C01P2002/86C01P2004/04C01P2004/51C01P2004/64
Inventor 张黎明傅超萍王梦莹沈君段小慧
Owner SUN YAT SEN UNIV
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