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High-efficiency composite biological agent for processing high-salt degradation-resistant organic wastewater and application of high-efficiency composite biological agent

A technology of compound biological bacteria and organic wastewater, applied in the directions of microorganisms, microorganisms, water pollutants, etc., can solve the problems of dehydration and inactivation of microorganisms, block intercellular pores, increase membrane pressure, etc., to restore normal levels, improve effluent quality, The effect of increasing resistance

Active Publication Date: 2017-03-22
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, high salinity and refractory organic wastewater has high salinity, and microorganisms will generally be dehydrated and inactivated; and the polymer in wastewater has a large molecular weight, complex structure, stable properties and is difficult to be decomposed by microorganisms. When it is deposited on the surface of microbial cell membranes, it will block the intercellular space Pores, leading to increased membrane pressure and membrane fouling, have a certain impact on microbial activity
Therefore, it is difficult to use biological methods to treat high-salt and refractory organic wastewater.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Example 1: the preparation method of composite microbial agent and the application in the treatment of drilling wastewater.

[0022] 1. Biological properties

[0023] Z-1 is a monococcus, Gram-negative bacteria. On solid medium, the colonies are round, white, smooth, moist and shiny, with neat edges and non-sticky.

[0024] P-3 is a coryneform, Gram-negative bacterium. On solid medium, the colonies are raised, with neat edges and glossy.

[0025] 2. To identify the 16S rDNA of strains Z-1 and P-3, the specific steps are as follows:

[0026] 1) Identification of strain Z-1 16S rDNA

[0027] Forward primer F1: 5'-AGAGTTTGATCCTGGCTCAG-3', reverse primer R1: 5'-GGTTACCTTGTTACGACTT-3', for DNA amplification (PCR). PCR reaction conditions: pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 58°C for 30 s, extension at 72°C for 90 s, 35 cycles, final extension at 72°C for 10 min, storage at 4°C. PCR products were separated by 0.8% agarose gel,...

example 2

[0061] Example 2 Composite bacterial agent treatment of a certain chemical wastewater

[0062] The difference from Example 1 is that the compound bacterial agent treats a certain chemical wastewater. The salt content of chemical wastewater is 2%, the conductivity is 26.7ms / cm, and the COD of raw water is 4960mg / L. The high-efficiency composite biological agent is used for aerobic treatment. The ratio of compound bacterial agent is: Z-1 is 30%, P-3 is 35%, salt-tolerant activated sludge is 35%, and the dosage of compound bacterial agent is 0.5‰. After 28 hours, the effluent reaches 387mg / L.

example 3

[0063] Example 3 Composite bacterial agent treatment of a certain chemical wastewater

[0064] The difference from Example 1 is that the compound bacterial agent treats a certain chemical wastewater. The salt content of wastewater from a chemical plant is 1.36%. The COD of the original system contacted with the aerobic tank was 2480mg / L, the residence time was 30h, and the effluent was 284mg / L. After being impacted, the microbial system collapsed, and the COD of the effluent was higher than that of the influent. Reached 3110mg / L. Half a month later, the COD remained high, and the original microbial system recovered slowly, and then began to continuously add high-efficiency compound biological agents. The ratio of compound microbial agent is: 30% for Z-1, 20% for P-3, 40% for salt-tolerant activated sludge, 0.1‰ per day, and 0.5‰ of growth promoter per day to promote growth. The agent provides COD about 100mg / L. After 2 days, the COD of the effluent began to decrease, and af...

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Abstract

The invention relates to a composite biological agent for processing high-salt degradation-resistant organic wastewater and application of the composite biological agent. The composite biological agent is composed of, by volume fraction, 15-45% of alcaligenes Z-1, 20-35% of sphingobacterium multivorum P-3 and 10-40% of salt-tolerance activated sludge. The organic wastewater with the salt content being 1-3% and the COD being 3200-7200 mg / L is subjected to aerobic treatment for 24-48 hours by the high-efficiency composite biological agent, so that the effluent COD is below 500 mg / L; and the organic wastewater with the COD being 800-3200 mg / L is subjected to aerobic treatment for 24-36 hours by the high-efficiency composite biological agent, so that the effluent COD is below 120 mg / L.

Description

technical field [0001] The invention belongs to the field of waste water treatment, and relates to a microbial compound bacterial agent for treating high-salt and refractory organic waste water, especially a high-efficiency compound for degrading organic waste water containing polyacrylamide, polysulfone, benzene and benzene homologues. Biological agent. Background technique [0002] my country's petroleum and chemical industry produces a large amount of high-salt and refractory organic wastewater every year. This type of wastewater often has the characteristics of high salt content, high polymer concentration, complex components, and refractory degradation. Due to the lack of technically feasible and economically reasonable treatment for a long time This method has caused a series of outstanding problems such as high investment and difficult treatment of enterprises, and the direct discharge of such wastewater will cause serious pollution to surface water, groundwater, soil,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05C12R1/01C02F101/30
CPCC02F3/34C02F2101/30C12N1/205C12R2001/01C12R2001/05
Inventor 杨勇霍莹郑贝贝张莹张艳芳付连超
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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