A kind of beautiful preparation method of cordyceps militaris wine
A technology of Cordyceps militaris and aesthetics, which is applied in the field of preparation of Cordyceps wine, can solve the problems of long cultivation period, uneven growth, poor shape and appearance of Cordyceps, and achieve the effect of stable and consistent properties, clear wine color and beautiful appearance
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Embodiment 1
[0046] The expanded cultivation of embodiment 1 Cordyceps bacterial classification
[0047] Incline culture: Inoculate the isolated and purified Cordyceps fungus into the inclined test tube, then put the inoculated inclined test tube into the incubator at 22°C, and cultivate for 7 days until the mycelium is full of the test tube;
[0048] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;
[0049]Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, a...
Embodiment 2
[0053] The expanded cultivation of embodiment 2 Cordyceps bacterial classification
[0054] Incline culture: Inoculate the isolated and purified Cordyceps militaris strains into inclined test tubes, and then put the inclined test tubes inoculated into the incubator at 22°C for 7 days until the mycelium is full of the test tubes;
[0055] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;
[0056] Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, a...
Embodiment 3
[0059] The expanded cultivation of embodiment 3 Cordyceps bacterial classification
[0060] Incline culture: Inoculate the isolated and purified Cordyceps fungus into the inclined test tube, then put the inoculated inclined test tube into the incubator at 22°C, and cultivate for 7 days until the mycelium is full of the test tube;
[0061] Seed tank culture: put 20L liquid medium in a 50L airlift seed tank, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C and keep the pressure for 30-45 minutes; after sterilization, when it is cooled to 20°C, put 4 bottles of the bacteria in the above-mentioned inclined test tube into the culture medium, the culture temperature is 22±1°C, and the ventilation volume is 1 : 0.5V / V min, holding pressure 4.903-7.0845×10 4 Pa, after 3 days of aerated culture, it can reach the logarithmic growth phase, and the...
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